Comparative Evaluation of Bone Repair with Four Different Bone Substitutes in Critical Size Defects
This study evaluated the osteoconductive potential of four biomaterials used to fill bone defects. For this, 24 male Albino rabbits were submitted to the creation of a bilateral 8 mm calvarial bone defect. The animals were divided into four groups—bovine hydroxyapatite, Bio-Oss® (BIO); Lumina-Bone P...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2020/5182845 |
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| author | Gustavo Grossi-Oliveira Leonardo P. Faverani Bruno Coelho Mendes Tárik Ocon Braga Polo Gabriel Cury Batista Mendes Valthierre Nunes de Lima Paulo Domingos Ribeiro Júnior Roberta Okamoto Osvaldo Magro-Filho |
| author_facet | Gustavo Grossi-Oliveira Leonardo P. Faverani Bruno Coelho Mendes Tárik Ocon Braga Polo Gabriel Cury Batista Mendes Valthierre Nunes de Lima Paulo Domingos Ribeiro Júnior Roberta Okamoto Osvaldo Magro-Filho |
| author_sort | Gustavo Grossi-Oliveira |
| collection | DOAJ |
| description | This study evaluated the osteoconductive potential of four biomaterials used to fill bone defects. For this, 24 male Albino rabbits were submitted to the creation of a bilateral 8 mm calvarial bone defect. The animals were divided into four groups—bovine hydroxyapatite, Bio-Oss® (BIO); Lumina-Bone Porous® (LBP); Bonefill® (BFL); and an alloplastic material, Clonos® (CLN)—and were euthanized at 14 and 40 days. The samples were subjected to histological and histometric analysis for newly formed bone area. Immunohistochemical analysis for Runt-related transcription factor 2 (Runx2), vascular endothelial growth factor (VEGF), and osteocalcin (OC) was performed. After statistical analysis, the CLN group showed greater new bone formation (NB) in both periods analyzed (p<0.05). At 14 days, the NB showed greater values in BIO in relation to LBP and BFL groups; however, after 40 days, the LBP group surpassed the results of BIO (p<0.001). The immunostaining showed a decrease in Runx2 intensity in BIO after 40 days, while it increased for LBP (p<0.05). The CLN showed increased OC compared to the other groups in both periods analyzed (p<0.05). Therefore, CLN showed the best osteoconductive behavior in critical defects in rabbit calvaria, and BFL showed the lowest osteoconductive property. |
| format | Article |
| id | doaj-art-15f66155657e411082e5b705a8aa77b0 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
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| series | International Journal of Biomaterials |
| spelling | doaj-art-15f66155657e411082e5b705a8aa77b02025-02-03T05:44:15ZengWileyInternational Journal of Biomaterials1687-87871687-87952020-01-01202010.1155/2020/51828455182845Comparative Evaluation of Bone Repair with Four Different Bone Substitutes in Critical Size DefectsGustavo Grossi-Oliveira0Leonardo P. Faverani1Bruno Coelho Mendes2Tárik Ocon Braga Polo3Gabriel Cury Batista Mendes4Valthierre Nunes de Lima5Paulo Domingos Ribeiro Júnior6Roberta Okamoto7Osvaldo Magro-Filho8Oral and Maxillofacial Surgery, Diagnostic and Surgery, Aracatuba School of Dentistry, Sao Paulo State University (UNESP), Aracatuba, Sao Paulo, BrazilOral and Maxillofacial Surgery, Diagnostic and Surgery, Aracatuba School of Dentistry, Sao Paulo State University (UNESP), Aracatuba, Sao Paulo, BrazilOral and Maxillofacial Surgery, Diagnostic and Surgery, Aracatuba School of Dentistry, Sao Paulo State University (UNESP), Aracatuba, Sao Paulo, BrazilOral and Maxillofacial Surgery, Diagnostic and Surgery, Aracatuba School of Dentistry, Sao Paulo State University (UNESP), Aracatuba, Sao Paulo, BrazilOral and Maxillofacial Surgery, Department of Oral Surgery, University of Sagrado Coracao, Bauru, Sao Paulo, BrazilOral and Maxillofacial Surgery, Diagnostic and Surgery, Aracatuba School of Dentistry, Sao Paulo State University (UNESP), Aracatuba, Sao Paulo, BrazilOral and Maxillofacial Surgery, Department of Oral Surgery, University of Sagrado Coracao, Bauru, Sao Paulo, BrazilHuman Anatomy, Department of Basic Sciences, Aracatuba School of Dentistry, Sao Paulo State University (UNESP), Aracatuba, Sao Paulo, BrazilOral and Maxillofacial Surgery, Diagnostic and Surgery, Aracatuba School of Dentistry, Sao Paulo State University (UNESP), Aracatuba, Sao Paulo, BrazilThis study evaluated the osteoconductive potential of four biomaterials used to fill bone defects. For this, 24 male Albino rabbits were submitted to the creation of a bilateral 8 mm calvarial bone defect. The animals were divided into four groups—bovine hydroxyapatite, Bio-Oss® (BIO); Lumina-Bone Porous® (LBP); Bonefill® (BFL); and an alloplastic material, Clonos® (CLN)—and were euthanized at 14 and 40 days. The samples were subjected to histological and histometric analysis for newly formed bone area. Immunohistochemical analysis for Runt-related transcription factor 2 (Runx2), vascular endothelial growth factor (VEGF), and osteocalcin (OC) was performed. After statistical analysis, the CLN group showed greater new bone formation (NB) in both periods analyzed (p<0.05). At 14 days, the NB showed greater values in BIO in relation to LBP and BFL groups; however, after 40 days, the LBP group surpassed the results of BIO (p<0.001). The immunostaining showed a decrease in Runx2 intensity in BIO after 40 days, while it increased for LBP (p<0.05). The CLN showed increased OC compared to the other groups in both periods analyzed (p<0.05). Therefore, CLN showed the best osteoconductive behavior in critical defects in rabbit calvaria, and BFL showed the lowest osteoconductive property.http://dx.doi.org/10.1155/2020/5182845 |
| spellingShingle | Gustavo Grossi-Oliveira Leonardo P. Faverani Bruno Coelho Mendes Tárik Ocon Braga Polo Gabriel Cury Batista Mendes Valthierre Nunes de Lima Paulo Domingos Ribeiro Júnior Roberta Okamoto Osvaldo Magro-Filho Comparative Evaluation of Bone Repair with Four Different Bone Substitutes in Critical Size Defects International Journal of Biomaterials |
| title | Comparative Evaluation of Bone Repair with Four Different Bone Substitutes in Critical Size Defects |
| title_full | Comparative Evaluation of Bone Repair with Four Different Bone Substitutes in Critical Size Defects |
| title_fullStr | Comparative Evaluation of Bone Repair with Four Different Bone Substitutes in Critical Size Defects |
| title_full_unstemmed | Comparative Evaluation of Bone Repair with Four Different Bone Substitutes in Critical Size Defects |
| title_short | Comparative Evaluation of Bone Repair with Four Different Bone Substitutes in Critical Size Defects |
| title_sort | comparative evaluation of bone repair with four different bone substitutes in critical size defects |
| url | http://dx.doi.org/10.1155/2020/5182845 |
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