Rational computational Design of new-Generation EGFR tyrosine kinase (EGFR-TK) inhibitors

A library of 45 novel compounds, derivatives of 2,3-diphenyl-2,3-dihydro-1H-quinazolin-4-one, were designed as potential EGFR inhibitors. This work describes in-silico study utilizing structure-based drug design (SBDD) and ligand-based drug design (LBDD) methodologies, incorporating Absorption, Dist...

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Main Authors: Chandraprakash Gond, Nikhil Kumar, Akanksha Mishra, Shivani Daksh, Anupama Datta, Anjani Kumar Tiwari
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Results in Chemistry
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Online Access:http://www.sciencedirect.com/science/article/pii/S221171562500222X
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author Chandraprakash Gond
Nikhil Kumar
Akanksha Mishra
Shivani Daksh
Anupama Datta
Anjani Kumar Tiwari
author_facet Chandraprakash Gond
Nikhil Kumar
Akanksha Mishra
Shivani Daksh
Anupama Datta
Anjani Kumar Tiwari
author_sort Chandraprakash Gond
collection DOAJ
description A library of 45 novel compounds, derivatives of 2,3-diphenyl-2,3-dihydro-1H-quinazolin-4-one, were designed as potential EGFR inhibitors. This work describes in-silico study utilizing structure-based drug design (SBDD) and ligand-based drug design (LBDD) methodologies, incorporating Absorption, Distribution, Metabolism, Excretion and Toxicity (ADMET) profiling, 3D-QSAR, molecular docking, and molecular dynamics (MD) simulations. ADMET profile of all the compounds were evaluated. A predictive 3D-QSAR model showed R(Alanazi et al., 20162), Rpred2, and Q2 values as 0.95, 0.62, and 0.52, respectively. The designed compounds showed binding affinities ranging from −6.9 to −8.4 kcal/mol when docked against the target protein (PDBID-6LUD). Top inhibitors included compounds 12, 13, 15, 26, 27, 28, 29, 30, 43, and 45 which demonstrated binding affinities more than −8.0 kcal/mol. Out of those, highest docking score was for compound 12 (−8.4 kcal/mol), surpassing the known anticancer drug Vandetanib (−8.0 kcal/mol). In addition, 100 ns MD simulations validated the stability of the protein-ligand complexes, confirming the potential of the selected compounds as potent EGFR inhibitor.
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spelling doaj-art-15ead91b183949d4b8cae4e73756b1b32025-08-20T02:09:18ZengElsevierResults in Chemistry2211-71562025-05-011510223910.1016/j.rechem.2025.102239Rational computational Design of new-Generation EGFR tyrosine kinase (EGFR-TK) inhibitorsChandraprakash Gond0Nikhil Kumar1Akanksha Mishra2Shivani Daksh3Anupama Datta4Anjani Kumar Tiwari5Department of Chemistry, Babasaheb Bhimrao Ambedkar University (A Central University), Lucknow 226025, Uttar Pradesh, IndiaInstitute of Nuclear Medicine and Allied Science, DRDO, Delhi 110054, India; Department of Chemistry, Indian Institute of Technology, Delhi 110016, IndiaDepartment of Chemistry, Babasaheb Bhimrao Ambedkar University (A Central University), Lucknow 226025, Uttar Pradesh, IndiaInstitute of Nuclear Medicine and Allied Science, DRDO, Delhi 110054, India; Department of Chemistry, Indian Institute of Technology, Delhi 110016, IndiaInstitute of Nuclear Medicine and Allied Science, DRDO, Delhi 110054, IndiaDepartment of Chemistry, Babasaheb Bhimrao Ambedkar University (A Central University), Lucknow 226025, Uttar Pradesh, India; Corresponding author.A library of 45 novel compounds, derivatives of 2,3-diphenyl-2,3-dihydro-1H-quinazolin-4-one, were designed as potential EGFR inhibitors. This work describes in-silico study utilizing structure-based drug design (SBDD) and ligand-based drug design (LBDD) methodologies, incorporating Absorption, Distribution, Metabolism, Excretion and Toxicity (ADMET) profiling, 3D-QSAR, molecular docking, and molecular dynamics (MD) simulations. ADMET profile of all the compounds were evaluated. A predictive 3D-QSAR model showed R(Alanazi et al., 20162), Rpred2, and Q2 values as 0.95, 0.62, and 0.52, respectively. The designed compounds showed binding affinities ranging from −6.9 to −8.4 kcal/mol when docked against the target protein (PDBID-6LUD). Top inhibitors included compounds 12, 13, 15, 26, 27, 28, 29, 30, 43, and 45 which demonstrated binding affinities more than −8.0 kcal/mol. Out of those, highest docking score was for compound 12 (−8.4 kcal/mol), surpassing the known anticancer drug Vandetanib (−8.0 kcal/mol). In addition, 100 ns MD simulations validated the stability of the protein-ligand complexes, confirming the potential of the selected compounds as potent EGFR inhibitor.http://www.sciencedirect.com/science/article/pii/S221171562500222XCADDDockingEGFR
spellingShingle Chandraprakash Gond
Nikhil Kumar
Akanksha Mishra
Shivani Daksh
Anupama Datta
Anjani Kumar Tiwari
Rational computational Design of new-Generation EGFR tyrosine kinase (EGFR-TK) inhibitors
Results in Chemistry
CADD
Docking
EGFR
title Rational computational Design of new-Generation EGFR tyrosine kinase (EGFR-TK) inhibitors
title_full Rational computational Design of new-Generation EGFR tyrosine kinase (EGFR-TK) inhibitors
title_fullStr Rational computational Design of new-Generation EGFR tyrosine kinase (EGFR-TK) inhibitors
title_full_unstemmed Rational computational Design of new-Generation EGFR tyrosine kinase (EGFR-TK) inhibitors
title_short Rational computational Design of new-Generation EGFR tyrosine kinase (EGFR-TK) inhibitors
title_sort rational computational design of new generation egfr tyrosine kinase egfr tk inhibitors
topic CADD
Docking
EGFR
url http://www.sciencedirect.com/science/article/pii/S221171562500222X
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