Experimental assessment and comparative analysis of efficacy for modified method of cellular therapy in chronic wounds on background of diabetes mellitus
Objective. Determination of efficacy of the cellular therapy modified method in chronic wounds on background of diabetes mellitus, comparing with standard and new methods of treatment in experiment. Materials and methods. Experimental investigations were conducted on 30 adult male rats of a Wista...
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Liga-Inform ltd.
2022-01-01
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| Series: | Клінічна хірургія |
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| Online Access: | https://hirurgiya.com.ua/index.php/journal/article/view/1001 |
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| author | S. M. Gramatiuk Yu. V. Ivanova I. A. Кryvoruchko M. M. Goloborodko K. V. Miasoiedov D. V. Mіnukhin O. M. Shevchenko |
| author_facet | S. M. Gramatiuk Yu. V. Ivanova I. A. Кryvoruchko M. M. Goloborodko K. V. Miasoiedov D. V. Mіnukhin O. M. Shevchenko |
| author_sort | S. M. Gramatiuk |
| collection | DOAJ |
| description | Objective. Determination of efficacy of the cellular therapy modified method in chronic wounds on background of diabetes mellitus, comparing with standard and new methods of treatment in experiment.
Materials and methods. Experimental investigations were conducted on 30 adult male rats of a Wistar line, in which diabetes mellitus was experimentally simulated. Full-thickness square wound was used as experimental pattern. In the animals of group A (control) the wounds were processed with 0.05% solution of chlorhexidine bigluconate and the hydrohel bandages put on (standard of treatment). To the group B animals photodynamic therapy was conducted, and processes of regeneration were stimulated with the help of the mesenchymal stem cells application. To the group C animals the wounds bottom and edges were infiltrated with suspension of autologous mesenchymal stem cells for active regeneration after conduction of photodynamic therapy.
Results. Best healing of the wounds while application of photodynamic therapy and injections of mesenchymal stem cells was noted: on the 5th day from the treatment beginning the wounds square reduction have appeared the most – 71.9%, while application of mesenchymal stem cells this index have constituted 61.6%, and while standard treatment – 36.0%.
Conclusion. Treatment of chronic wounds on background of diabetes mellitus, using photodynamic therapy and injections of mesenchymal stem cells, have demonstrated essentially better results, than after standard method. Application of mesenchymal stem cells into the wound, the results of which were compared with results of their injectional introduction, may be recommended as alternative, if immediate injections of mesenchymal stem cells into the tissues over the wound are impossible. |
| format | Article |
| id | doaj-art-15e4e6cd1f5146e1990dd264eed2ff4e |
| institution | DOAJ |
| issn | 0023-2130 2522-1396 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Liga-Inform ltd. |
| record_format | Article |
| series | Клінічна хірургія |
| spelling | doaj-art-15e4e6cd1f5146e1990dd264eed2ff4e2025-08-20T03:15:16ZengLiga-Inform ltd.Клінічна хірургія0023-21302522-13962022-01-018811-12687410.26779/2522-1396.2021.11-12.681001Experimental assessment and comparative analysis of efficacy for modified method of cellular therapy in chronic wounds on background of diabetes mellitusS. M. Gramatiuk0Yu. V. Ivanova1I. A. Кryvoruchko2M. M. Goloborodko3K. V. Miasoiedov4D. V. Mіnukhin5O. M. Shevchenko6Іnstitute of Cellular Biorehabilitation, KharkivKharkiv National Medical UniversityKharkiv National Medical UniversityKharkiv National Medical UniversityKharkiv National Medical UniversityKharkiv National Medical UniversityKharkiv National Medical UniversityObjective. Determination of efficacy of the cellular therapy modified method in chronic wounds on background of diabetes mellitus, comparing with standard and new methods of treatment in experiment. Materials and methods. Experimental investigations were conducted on 30 adult male rats of a Wistar line, in which diabetes mellitus was experimentally simulated. Full-thickness square wound was used as experimental pattern. In the animals of group A (control) the wounds were processed with 0.05% solution of chlorhexidine bigluconate and the hydrohel bandages put on (standard of treatment). To the group B animals photodynamic therapy was conducted, and processes of regeneration were stimulated with the help of the mesenchymal stem cells application. To the group C animals the wounds bottom and edges were infiltrated with suspension of autologous mesenchymal stem cells for active regeneration after conduction of photodynamic therapy. Results. Best healing of the wounds while application of photodynamic therapy and injections of mesenchymal stem cells was noted: on the 5th day from the treatment beginning the wounds square reduction have appeared the most – 71.9%, while application of mesenchymal stem cells this index have constituted 61.6%, and while standard treatment – 36.0%. Conclusion. Treatment of chronic wounds on background of diabetes mellitus, using photodynamic therapy and injections of mesenchymal stem cells, have demonstrated essentially better results, than after standard method. Application of mesenchymal stem cells into the wound, the results of which were compared with results of their injectional introduction, may be recommended as alternative, if immediate injections of mesenchymal stem cells into the tissues over the wound are impossible.https://hirurgiya.com.ua/index.php/journal/article/view/1001chronic woundscellular therapydiabetes mellitusеxperiment |
| spellingShingle | S. M. Gramatiuk Yu. V. Ivanova I. A. Кryvoruchko M. M. Goloborodko K. V. Miasoiedov D. V. Mіnukhin O. M. Shevchenko Experimental assessment and comparative analysis of efficacy for modified method of cellular therapy in chronic wounds on background of diabetes mellitus Клінічна хірургія chronic wounds cellular therapy diabetes mellitus еxperiment |
| title | Experimental assessment and comparative analysis of efficacy for modified method of cellular therapy in chronic wounds on background of diabetes mellitus |
| title_full | Experimental assessment and comparative analysis of efficacy for modified method of cellular therapy in chronic wounds on background of diabetes mellitus |
| title_fullStr | Experimental assessment and comparative analysis of efficacy for modified method of cellular therapy in chronic wounds on background of diabetes mellitus |
| title_full_unstemmed | Experimental assessment and comparative analysis of efficacy for modified method of cellular therapy in chronic wounds on background of diabetes mellitus |
| title_short | Experimental assessment and comparative analysis of efficacy for modified method of cellular therapy in chronic wounds on background of diabetes mellitus |
| title_sort | experimental assessment and comparative analysis of efficacy for modified method of cellular therapy in chronic wounds on background of diabetes mellitus |
| topic | chronic wounds cellular therapy diabetes mellitus еxperiment |
| url | https://hirurgiya.com.ua/index.php/journal/article/view/1001 |
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