Protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer culture

Protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer culture

Summary: Human pluripotent stem cells (hPSCs) provide a powerful platform for generating hematopoietic progenitor cells (HPCs) and investigating hematopoietic development. Here, we present a protocol for maintaining hPSCs and inducing their differentiation into HPCs through the endothelial-to-hemato...

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Bibliographic Details
Main Authors: Shaokang Mo, Kengyuan Qu, Jun Shen, Kuangyu Yen
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
cell biology
cell culture
developmental biology
stem cells
cell differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672400710X
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