Profiles of aroma volatile components in textured vegetable proteins using headspace solid phase microextraction-gas chromatography-mass spectrometry

Textured vegetable protein (TVP) is a significant alternative to meat, with its primary raw materials being soybeans, peas, rice, and wheat proteins. While advancements in technology have successfully replicated the unique texture of meat in plant-based proteins, research on the aroma profiles of th...

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Main Authors: Geon-Woo Park, Kyung-Ho Park, Sang-Gu Kim, Sang-Yun Lee
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Current Research in Food Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S2665927125000309
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author Geon-Woo Park
Kyung-Ho Park
Sang-Gu Kim
Sang-Yun Lee
author_facet Geon-Woo Park
Kyung-Ho Park
Sang-Gu Kim
Sang-Yun Lee
author_sort Geon-Woo Park
collection DOAJ
description Textured vegetable protein (TVP) is a significant alternative to meat, with its primary raw materials being soybeans, peas, rice, and wheat proteins. While advancements in technology have successfully replicated the unique texture of meat in plant-based proteins, research on the aroma profiles of these key raw materials remains limited. The subtle differences in aroma between meat and meat substitutes are yet to be fully addressed. In this study, we employed headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS), a specialized technique for the analysis of volatile aromatic compounds, to examine the volatile profiles of soybean, pea, rice, and wheat proteins. The identified volatile compounds included alcohols, aldehydes, carboxylic acids, ethers, furans, indoles, ketones, phenols, pyrans, and sulfur compounds. Based on prior research, eight compounds (hexanal, nonanal, 2-nonenal, 3-methylbutanal, benzaldehyde, 1-octen-3-ol, 3-octen-2-one, and 2-pentylfuran) were classified as off-flavors. Hexanal, a key marker, was found in the following order: rice showed the highest levels, followed by soybeans, peas, and wheat. Other major volatile components exhibited distinct ratios across the samples. These findings could assist in refining the next generation of TVPs and minimizing aroma heterogeneity.
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issn 2665-9271
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publishDate 2025-01-01
publisher Elsevier
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series Current Research in Food Science
spelling doaj-art-15da73552fbc49bfa9b58775edc347ce2025-08-20T03:20:05ZengElsevierCurrent Research in Food Science2665-92712025-01-011010099910.1016/j.crfs.2025.100999Profiles of aroma volatile components in textured vegetable proteins using headspace solid phase microextraction-gas chromatography-mass spectrometryGeon-Woo Park0Kyung-Ho Park1Sang-Gu Kim2Sang-Yun Lee3Corresponding author. Tel.: +82-43-903-3892; Fax: +82-2-6499-0131; Corporate Technology Office, Pulmuone. Co. Ltd., 29, Osongsaengmyeong 10-ro, Osong-eup, Heungdeok-gu, Cheongju, 28220, Republic of KoreaCorporate Technology Office, Pulmuone. Co. Ltd., 29, Osongsaengmyeong 10-ro, Osong-eup, Heungdeok-gu, Cheongju, 28220, Republic of KoreaCorporate Technology Office, Pulmuone. Co. Ltd., 29, Osongsaengmyeong 10-ro, Osong-eup, Heungdeok-gu, Cheongju, 28220, Republic of KoreaCorporate Technology Office, Pulmuone. Co. Ltd., 29, Osongsaengmyeong 10-ro, Osong-eup, Heungdeok-gu, Cheongju, 28220, Republic of KoreaTextured vegetable protein (TVP) is a significant alternative to meat, with its primary raw materials being soybeans, peas, rice, and wheat proteins. While advancements in technology have successfully replicated the unique texture of meat in plant-based proteins, research on the aroma profiles of these key raw materials remains limited. The subtle differences in aroma between meat and meat substitutes are yet to be fully addressed. In this study, we employed headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS), a specialized technique for the analysis of volatile aromatic compounds, to examine the volatile profiles of soybean, pea, rice, and wheat proteins. The identified volatile compounds included alcohols, aldehydes, carboxylic acids, ethers, furans, indoles, ketones, phenols, pyrans, and sulfur compounds. Based on prior research, eight compounds (hexanal, nonanal, 2-nonenal, 3-methylbutanal, benzaldehyde, 1-octen-3-ol, 3-octen-2-one, and 2-pentylfuran) were classified as off-flavors. Hexanal, a key marker, was found in the following order: rice showed the highest levels, followed by soybeans, peas, and wheat. Other major volatile components exhibited distinct ratios across the samples. These findings could assist in refining the next generation of TVPs and minimizing aroma heterogeneity.http://www.sciencedirect.com/science/article/pii/S2665927125000309Textured vegetable proteinAroma volatile componentHS-SPME-GC-MSsoybean proteinpea proteinrice protein
spellingShingle Geon-Woo Park
Kyung-Ho Park
Sang-Gu Kim
Sang-Yun Lee
Profiles of aroma volatile components in textured vegetable proteins using headspace solid phase microextraction-gas chromatography-mass spectrometry
Current Research in Food Science
Textured vegetable protein
Aroma volatile component
HS-SPME-GC-MS
soybean protein
pea protein
rice protein
title Profiles of aroma volatile components in textured vegetable proteins using headspace solid phase microextraction-gas chromatography-mass spectrometry
title_full Profiles of aroma volatile components in textured vegetable proteins using headspace solid phase microextraction-gas chromatography-mass spectrometry
title_fullStr Profiles of aroma volatile components in textured vegetable proteins using headspace solid phase microextraction-gas chromatography-mass spectrometry
title_full_unstemmed Profiles of aroma volatile components in textured vegetable proteins using headspace solid phase microextraction-gas chromatography-mass spectrometry
title_short Profiles of aroma volatile components in textured vegetable proteins using headspace solid phase microextraction-gas chromatography-mass spectrometry
title_sort profiles of aroma volatile components in textured vegetable proteins using headspace solid phase microextraction gas chromatography mass spectrometry
topic Textured vegetable protein
Aroma volatile component
HS-SPME-GC-MS
soybean protein
pea protein
rice protein
url http://www.sciencedirect.com/science/article/pii/S2665927125000309
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