Toxoplasma gondii infection in aborted women and sheep in the governorates of El-Beheira and Alexandria, Egypt: A sero-immunological and molecular study
Abortion results from a Toxoplasma gondii infection in both humans and mammals, causing substantial losses in terms of quality-adjusted life years and financial consequences. The incidence of toxoplasmosis in the current study in aborted women and sheep was determined using serological, immunologica...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Arabic |
| Published: |
University of Mosul, College of Veterinary Medicine
2025-07-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.vetmedmosul.com/article_187626_d70c3a929b35e8ced1ff67e488a2bfc3.pdf |
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| Summary: | Abortion results from a Toxoplasma gondii infection in both humans and mammals, causing substantial losses in terms of quality-adjusted life years and financial consequences. The incidence of toxoplasmosis in the current study in aborted women and sheep was determined using serological, immunological, and molecular assays. Blood samples were taken from sheep that appeared to be healthy and from women who had aborted in the governorates of Alexandria and El-Beheira on the west coast of Egypt. The specific immunogenetic bands in the crude tachyzoite antigens from both RH and local T. gondii strains were characterized using SDS-PAGE and immunoblotting assays. An indirect ELISA was performed to detect the prevalence in aborted women and sheep. The confirmatory molecular identification of toxoplasmosis was adopted using conventional polymerase chain reaction (cPCR). The results revealed that the two RH and local T. gondii antigens showed extensive electrophoretic similarity and identified two common immunogenic bands (67 and 58 kDa) in naturally infected human and sheep sera with T. gondii. The rate of toxoplasmosis sero-prevalence recorded by ELISA in aborted women was 60.8% (76/125), while in sheep it was 67.3% (66/98). Verified PCR results of toxoplasmosis in aborted women and sheep revealed the expected positive bands, 194 bp and 161 bp using B1 and Tox-9 & Tox-11 gene primers, respectively. This study concluded that the higher T. gondii prevalence might be responsible for abortion and economic losses in humans and sheep in our studied region. These findings reinforce the necessity of more epidemiological studies on toxoplasmosis and public health education initiatives. |
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| ISSN: | 1607-3894 2071-1255 |