Biopatinas on Peperino Stone: Three Eco-Friendly Methods for Their Control and Multi-Technique Approach to Evaluate Their Efficacy
In restoration practice, direct methods become necessary when indirect methods fail and when aesthetic, chemical, or physical biodeteriorative effects threaten the integrity and legibility of the artifact. More effective methods that prioritize the health of workers and the environment are essential...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/2/375 |
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| Summary: | In restoration practice, direct methods become necessary when indirect methods fail and when aesthetic, chemical, or physical biodeteriorative effects threaten the integrity and legibility of the artifact. More effective methods that prioritize the health of workers and the environment are essential for the outdoor stone monument’s conservation. Although several low-impact methods have been proposed, more case studies are needed to address different biopatina types, products, and lithic substrates. Within the COLLINE Project we focused on peperino, a dark volcanic stone widely used in central Italy since the 7th century BCE, because it has been poorly investigated in terms of diversity of biodeteriogens and low-impact methods for their removal. Direct observation, culture methods, and molecular identification have been applied for the identification of biodeteriogens with particular attention to black meristematic fungi. Three low-impact products, namely a dimethyl sulfoxide (DMSO)-based gel, BioTersus<sup>®</sup> (essential oil-based), and Nasier (enzyme-based) were tested in <i>ex situ</i> (on a colonized slab) and <i>in situ</i> trials (on the pulpit of the S. Francesco alla Rocca Basilica, Viterbo, Italy). Three analytical methods, namely reflectance spectroradiometry, laser-induced fluorescence (LIF), and hypercolorimetric multispectral imaging (HMI) were used to test the cleaning efficacy. Results evidenced the strong influence of direct irradiation and water availability in the balance and distribution of phototrophs, fungi, and lichens. The low-impact cleaning methods, particularly the DMSO-based gel and BioTersus<sup>®</sup>, effectively remove biodeteriogens from peperino stone while preserving its integrity, offering sustainable solutions for cultural heritage conservation. The instrumental analyses showed that reflectance spectroradiometry and LIF effectively validated the cleaning efficacy, albeit with different sensibility, while HMI, despite spatial constraints, confirmed the three tested cleaning methods do not interfere with peperino stone. |
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| ISSN: | 2076-2607 |