Identification of a novel aminoglycoside nucleotidyltransferase ANT(3″)-Ic from Citrobacter telavivum S24
Abstract Understanding the antibiotic resistance profile of the emerging pathogen Citrobacter telavivum S24 is important for clinical care, surveillance, and research. C. telavivum S24, a strain that exhibits resistance to both streptomycin and spectinomycin, was isolated from the soil at a broiler...
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BMC
2025-08-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-025-04282-z |
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| author | Junwan Lu Mei Zhu Jingzeng Ye Leyao Xu Chunhan Song Kewei Li Qiyu Bao Xi Lin |
| author_facet | Junwan Lu Mei Zhu Jingzeng Ye Leyao Xu Chunhan Song Kewei Li Qiyu Bao Xi Lin |
| author_sort | Junwan Lu |
| collection | DOAJ |
| description | Abstract Understanding the antibiotic resistance profile of the emerging pathogen Citrobacter telavivum S24 is important for clinical care, surveillance, and research. C. telavivum S24, a strain that exhibits resistance to both streptomycin and spectinomycin, was isolated from the soil at a broiler chicken farm. The strain was identified by whole-genome sequencing and average nucleotide identity analyses. Annotation of the resistance genes revealed that a novel aminoglycoside resistance gene, designated ant(3″)-Ic, is located on the chromosome of C. telavivum S24. This gene exhibits 52% similarity with the previously functionally characterized resistance gene aadA23. It distinguishes itself from ant(3″)-Ia and ant(3″)-Ib genes, and represents an independent branch. The ant(3″)-Ic gene is recognized as an inherent resistance gene of C. telavivum within a conserved genetic environment. The product of the ant(3″)-Ic gene is the aminoglycoside nucleotidyltransferase ANT(3″)-Ic, which has a theoretical pI of 5.05 and a molecular mass of 29 kDa. ANT(3”)-Ic inactivates streptomycin and spectinomycin with the K m of 46.13 and 115.90 µM, respectively. The discovery of the novel antibiotic resistance gene ant(3″)-Ic and the functional characterization of ANT(3”)-Ic will facilitate more targeted surveillance and treatment strategies for preventing and controlling zoonotic pathogens of zoonotic diseases. |
| format | Article |
| id | doaj-art-15cbee87c9314c8188ff98a0870f77f1 |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj-art-15cbee87c9314c8188ff98a0870f77f12025-08-24T11:11:04ZengBMCBMC Microbiology1471-21802025-08-0125111210.1186/s12866-025-04282-zIdentification of a novel aminoglycoside nucleotidyltransferase ANT(3″)-Ic from Citrobacter telavivum S24Junwan Lu0Mei Zhu1Jingzeng Ye2Leyao Xu3Chunhan Song4Kewei Li5Qiyu Bao6Xi Lin7Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical UniversityDepartment of Clinical Laboratory, Zhejiang HospitalKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical UniversityKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical UniversityMedical Molecular Biology Laboratory, School of Medicine, Jinhua University of Vocational TechnologyKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical UniversityKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical UniversityKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical UniversityAbstract Understanding the antibiotic resistance profile of the emerging pathogen Citrobacter telavivum S24 is important for clinical care, surveillance, and research. C. telavivum S24, a strain that exhibits resistance to both streptomycin and spectinomycin, was isolated from the soil at a broiler chicken farm. The strain was identified by whole-genome sequencing and average nucleotide identity analyses. Annotation of the resistance genes revealed that a novel aminoglycoside resistance gene, designated ant(3″)-Ic, is located on the chromosome of C. telavivum S24. This gene exhibits 52% similarity with the previously functionally characterized resistance gene aadA23. It distinguishes itself from ant(3″)-Ia and ant(3″)-Ib genes, and represents an independent branch. The ant(3″)-Ic gene is recognized as an inherent resistance gene of C. telavivum within a conserved genetic environment. The product of the ant(3″)-Ic gene is the aminoglycoside nucleotidyltransferase ANT(3″)-Ic, which has a theoretical pI of 5.05 and a molecular mass of 29 kDa. ANT(3”)-Ic inactivates streptomycin and spectinomycin with the K m of 46.13 and 115.90 µM, respectively. The discovery of the novel antibiotic resistance gene ant(3″)-Ic and the functional characterization of ANT(3”)-Ic will facilitate more targeted surveillance and treatment strategies for preventing and controlling zoonotic pathogens of zoonotic diseases.https://doi.org/10.1186/s12866-025-04282-zC. telavivumAminoglycoside nucleotidyltransferaseANT(3″)-IcWhole-genome sequencingAminoglycoside resistance |
| spellingShingle | Junwan Lu Mei Zhu Jingzeng Ye Leyao Xu Chunhan Song Kewei Li Qiyu Bao Xi Lin Identification of a novel aminoglycoside nucleotidyltransferase ANT(3″)-Ic from Citrobacter telavivum S24 BMC Microbiology C. telavivum Aminoglycoside nucleotidyltransferase ANT(3″)-Ic Whole-genome sequencing Aminoglycoside resistance |
| title | Identification of a novel aminoglycoside nucleotidyltransferase ANT(3″)-Ic from Citrobacter telavivum S24 |
| title_full | Identification of a novel aminoglycoside nucleotidyltransferase ANT(3″)-Ic from Citrobacter telavivum S24 |
| title_fullStr | Identification of a novel aminoglycoside nucleotidyltransferase ANT(3″)-Ic from Citrobacter telavivum S24 |
| title_full_unstemmed | Identification of a novel aminoglycoside nucleotidyltransferase ANT(3″)-Ic from Citrobacter telavivum S24 |
| title_short | Identification of a novel aminoglycoside nucleotidyltransferase ANT(3″)-Ic from Citrobacter telavivum S24 |
| title_sort | identification of a novel aminoglycoside nucleotidyltransferase ant 3 ic from citrobacter telavivum s24 |
| topic | C. telavivum Aminoglycoside nucleotidyltransferase ANT(3″)-Ic Whole-genome sequencing Aminoglycoside resistance |
| url | https://doi.org/10.1186/s12866-025-04282-z |
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