Diploid chromosome-level genome assembly and annotation for Lycorma delicatula

Abstract The spotted lanternfly (Lycorma delicatula) is a planthopper species (Hemiptera: Fulgoridae) native to China but invasive in South Korea, Japan, and the United States where it is a significant threat to agriculture. Genomic resources are critical to both management of this species and under...

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Main Authors: Anthony A. Snead, Fang Meng, Nicolas Largotta, Kristin M. Winchell, Brenna A. Levine
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-04854-8
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author Anthony A. Snead
Fang Meng
Nicolas Largotta
Kristin M. Winchell
Brenna A. Levine
author_facet Anthony A. Snead
Fang Meng
Nicolas Largotta
Kristin M. Winchell
Brenna A. Levine
author_sort Anthony A. Snead
collection DOAJ
description Abstract The spotted lanternfly (Lycorma delicatula) is a planthopper species (Hemiptera: Fulgoridae) native to China but invasive in South Korea, Japan, and the United States where it is a significant threat to agriculture. Genomic resources are critical to both management of this species and understanding the genomic characteristics of successful invaders. We report an annotated, haplotype-phased, chromosome-level genome assembly for the spotted lanternfly using PacBio long-read sequencing, Hi-C technology, and RNA-seq. The 2.2 Gbp genome comprises 13 chromosomes, and whole genome resequencing of eighty-two adults indicated chromosome four as the sex chromosome and a corresponding XO sex-determination system. We identified over 12,000 protein-coding genes and performed functional annotation, facilitating the identification of candidate genes that may hold importance for spotted lanternfly control. The assemblies and annotations were highly complete with over 96% of BUSCO genes complete regardless of the database (i.e., Eukaryota, Arthropoda, Insecta). This reference-quality genome will serve as an important resource for development and optimization of management practices for the spotted lanternfly and invasive species genomics as a whole.
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spelling doaj-art-15ca90ee2e6f411eaff7d8b56ea075c62025-08-20T02:08:08ZengNature PortfolioScientific Data2052-44632025-04-0112111110.1038/s41597-025-04854-8Diploid chromosome-level genome assembly and annotation for Lycorma delicatulaAnthony A. Snead0Fang Meng1Nicolas Largotta2Kristin M. Winchell3Brenna A. Levine4Department of Biology, New York UniversityDepartment of Biology, New York UniversityDepartment of Biology, Kean UniversityDepartment of Biology, New York UniversityDepartment of Biology, Kean UniversityAbstract The spotted lanternfly (Lycorma delicatula) is a planthopper species (Hemiptera: Fulgoridae) native to China but invasive in South Korea, Japan, and the United States where it is a significant threat to agriculture. Genomic resources are critical to both management of this species and understanding the genomic characteristics of successful invaders. We report an annotated, haplotype-phased, chromosome-level genome assembly for the spotted lanternfly using PacBio long-read sequencing, Hi-C technology, and RNA-seq. The 2.2 Gbp genome comprises 13 chromosomes, and whole genome resequencing of eighty-two adults indicated chromosome four as the sex chromosome and a corresponding XO sex-determination system. We identified over 12,000 protein-coding genes and performed functional annotation, facilitating the identification of candidate genes that may hold importance for spotted lanternfly control. The assemblies and annotations were highly complete with over 96% of BUSCO genes complete regardless of the database (i.e., Eukaryota, Arthropoda, Insecta). This reference-quality genome will serve as an important resource for development and optimization of management practices for the spotted lanternfly and invasive species genomics as a whole.https://doi.org/10.1038/s41597-025-04854-8
spellingShingle Anthony A. Snead
Fang Meng
Nicolas Largotta
Kristin M. Winchell
Brenna A. Levine
Diploid chromosome-level genome assembly and annotation for Lycorma delicatula
Scientific Data
title Diploid chromosome-level genome assembly and annotation for Lycorma delicatula
title_full Diploid chromosome-level genome assembly and annotation for Lycorma delicatula
title_fullStr Diploid chromosome-level genome assembly and annotation for Lycorma delicatula
title_full_unstemmed Diploid chromosome-level genome assembly and annotation for Lycorma delicatula
title_short Diploid chromosome-level genome assembly and annotation for Lycorma delicatula
title_sort diploid chromosome level genome assembly and annotation for lycorma delicatula
url https://doi.org/10.1038/s41597-025-04854-8
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AT kristinmwinchell diploidchromosomelevelgenomeassemblyandannotationforlycormadelicatula
AT brennaalevine diploidchromosomelevelgenomeassemblyandannotationforlycormadelicatula