Extracellular vesicles from adipose-derived mesenchymal stem cells alleviate acute lung injury via the CBL/AMPK signaling pathway
Abstract Background Acute lung injury (ALI) which is caused by Staphylococcus aureus (SA), is a serious lung disease that threatens human health. Although some current treatments are effective in alleviating ALI, they still have a significant mortality rate. At present, adipose-derived mesenchymal s...
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BMC
2025-03-01
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| Series: | BMC Biology |
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| Online Access: | https://doi.org/10.1186/s12915-025-02178-y |
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| author | Yan Xiong Lulu Wang Bohao Li Beibei Fu Zhou Sha Jin Liu Rong Tian Rui Yao Feng Lin Zixuan Cong Yongliang Du Xiaoyuan Lin Haibo Wu |
| author_facet | Yan Xiong Lulu Wang Bohao Li Beibei Fu Zhou Sha Jin Liu Rong Tian Rui Yao Feng Lin Zixuan Cong Yongliang Du Xiaoyuan Lin Haibo Wu |
| author_sort | Yan Xiong |
| collection | DOAJ |
| description | Abstract Background Acute lung injury (ALI) which is caused by Staphylococcus aureus (SA), is a serious lung disease that threatens human health. Although some current treatments are effective in alleviating ALI, they still have a significant mortality rate. At present, adipose-derived mesenchymal stem cells (ADSCs)-derived extracellular vesicles (EVs) have been investigated for the treatment of various diseases. Here, we examined the role of ADSCs-derived EVs in regulating apoptosis and inflammation during ALI. Results We showed that ADSCs and ADSCs-derived EVs supplementation could improve lung injury, restore mitochondrial function, and inhibit inflammation and apoptosis in ALI mice. Furthermore, miR-320a was present in EVs derived from ADSCs, and it can be transferred into lung tissue. In vitro, Casitas B-lineage lymphoma (CBL) expression was inhibited by miR-320a mimics. Finally, we found that miR-320a alleviated mitochondrial damage, inflammation, and apoptosis via the CBL/AMPK/JNK pathway. Conclusions In conclusion, EVs from ADSCs could alleviate ALI via the CBL/AMPK signaling pathway. Therefore, the purpose of our study was to investigate the application of ADSC-derived EVs in mitigating ALI by modulating metabolic processes. |
| format | Article |
| id | doaj-art-1588cb7aebaf41d7af5d30a184dbae05 |
| institution | OA Journals |
| issn | 1741-7007 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biology |
| spelling | doaj-art-1588cb7aebaf41d7af5d30a184dbae052025-08-20T01:54:20ZengBMCBMC Biology1741-70072025-03-0123112110.1186/s12915-025-02178-yExtracellular vesicles from adipose-derived mesenchymal stem cells alleviate acute lung injury via the CBL/AMPK signaling pathwayYan Xiong0Lulu Wang1Bohao Li2Beibei Fu3Zhou Sha4Jin Liu5Rong Tian6Rui Yao7Feng Lin8Zixuan Cong9Yongliang Du10Xiaoyuan Lin11Haibo Wu12School of Life Sciences, Chongqing UniversitySchool of Life Sciences, Chongqing UniversitySchool of Life Sciences, Chongqing UniversitySchool of Life Sciences, Chongqing UniversitySchool of Life Sciences, Chongqing UniversitySchool of Pharmaceutical Sciences, Chongqing UniversityDepartment of Pathology, Chongqing Hygeia HospitalDepartment of Pathology, Chongqing Hygeia HospitalSchool of Life Sciences, Chongqing UniversitySchool of Life Sciences, Chongqing UniversitySchool of Life Sciences, Chongqing UniversityDepartment of Clinical Microbiology and Immunology, College of Pharmacy and Medical Laboratory, Army Medical University (Third Military Medical University)School of Life Sciences, Chongqing UniversityAbstract Background Acute lung injury (ALI) which is caused by Staphylococcus aureus (SA), is a serious lung disease that threatens human health. Although some current treatments are effective in alleviating ALI, they still have a significant mortality rate. At present, adipose-derived mesenchymal stem cells (ADSCs)-derived extracellular vesicles (EVs) have been investigated for the treatment of various diseases. Here, we examined the role of ADSCs-derived EVs in regulating apoptosis and inflammation during ALI. Results We showed that ADSCs and ADSCs-derived EVs supplementation could improve lung injury, restore mitochondrial function, and inhibit inflammation and apoptosis in ALI mice. Furthermore, miR-320a was present in EVs derived from ADSCs, and it can be transferred into lung tissue. In vitro, Casitas B-lineage lymphoma (CBL) expression was inhibited by miR-320a mimics. Finally, we found that miR-320a alleviated mitochondrial damage, inflammation, and apoptosis via the CBL/AMPK/JNK pathway. Conclusions In conclusion, EVs from ADSCs could alleviate ALI via the CBL/AMPK signaling pathway. Therefore, the purpose of our study was to investigate the application of ADSC-derived EVs in mitigating ALI by modulating metabolic processes.https://doi.org/10.1186/s12915-025-02178-yALIEVsMiR-320aAMPKCBLEnergy metabolism |
| spellingShingle | Yan Xiong Lulu Wang Bohao Li Beibei Fu Zhou Sha Jin Liu Rong Tian Rui Yao Feng Lin Zixuan Cong Yongliang Du Xiaoyuan Lin Haibo Wu Extracellular vesicles from adipose-derived mesenchymal stem cells alleviate acute lung injury via the CBL/AMPK signaling pathway BMC Biology ALI EVs MiR-320a AMPK CBL Energy metabolism |
| title | Extracellular vesicles from adipose-derived mesenchymal stem cells alleviate acute lung injury via the CBL/AMPK signaling pathway |
| title_full | Extracellular vesicles from adipose-derived mesenchymal stem cells alleviate acute lung injury via the CBL/AMPK signaling pathway |
| title_fullStr | Extracellular vesicles from adipose-derived mesenchymal stem cells alleviate acute lung injury via the CBL/AMPK signaling pathway |
| title_full_unstemmed | Extracellular vesicles from adipose-derived mesenchymal stem cells alleviate acute lung injury via the CBL/AMPK signaling pathway |
| title_short | Extracellular vesicles from adipose-derived mesenchymal stem cells alleviate acute lung injury via the CBL/AMPK signaling pathway |
| title_sort | extracellular vesicles from adipose derived mesenchymal stem cells alleviate acute lung injury via the cbl ampk signaling pathway |
| topic | ALI EVs MiR-320a AMPK CBL Energy metabolism |
| url | https://doi.org/10.1186/s12915-025-02178-y |
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