A novel plasma circular RNA circFARSA is a potential biomarker for non‐small cell lung cancer
Abstract Emerging evidence indicates that circular RNAs (circRNAs) are implicated in cancer development. This study aimed to evaluate whether circulating circRNAs may serve as novel biomarkers for non‐small cell lung cancer (NSCLC). We used RNA sequencing (RNA‐seq) and quantitative real‐time PCR to...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-06-01
|
| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.1514 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849710040996380672 |
|---|---|
| author | Dong Hang Jing Zhou Na Qin Wen Zhou Hongxia Ma Guangfu Jin Zhibin Hu Juncheng Dai Hongbing Shen |
| author_facet | Dong Hang Jing Zhou Na Qin Wen Zhou Hongxia Ma Guangfu Jin Zhibin Hu Juncheng Dai Hongbing Shen |
| author_sort | Dong Hang |
| collection | DOAJ |
| description | Abstract Emerging evidence indicates that circular RNAs (circRNAs) are implicated in cancer development. This study aimed to evaluate whether circulating circRNAs may serve as novel biomarkers for non‐small cell lung cancer (NSCLC). We used RNA sequencing (RNA‐seq) and quantitative real‐time PCR to explore cancer‐related circRNAs. Bioinformatics and functional analyses were performed to reveal biological effects of circRNAs on lung cancer cells. A total of 5471 distinct circRNAs were identified by total RNA‐seq, in which 185 were differentially expressed between cancerous and adjacent normal tissues. A circRNA derived from exon 5–7 of the FARSA gene, termed circFARSA, was observed to increase in cancerous tissues (P = 0.016), and was more abundant in patients’ plasma than controls (P < 0.001). Overexpression of circFARSA in A549 cell line significantly promoted cell migration and invasion. In silico analysis suggested that circFARSA might sponge miR‐330‐5p and miR‐326, thereby relieving their inhibitory effects on oncogene fatty acid synthase. Summarily, this study revealed circRNA profile of NSCLC for the first time and provided the evidence of plasma circFARSA as a potential noninvasive biomarker for this malignancy. |
| format | Article |
| id | doaj-art-1588304639e64f5aaddaede0daa2fe95 |
| institution | DOAJ |
| issn | 2045-7634 |
| language | English |
| publishDate | 2018-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-1588304639e64f5aaddaede0daa2fe952025-08-20T03:15:04ZengWileyCancer Medicine2045-76342018-06-01762783279110.1002/cam4.1514A novel plasma circular RNA circFARSA is a potential biomarker for non‐small cell lung cancerDong Hang0Jing Zhou1Na Qin2Wen Zhou3Hongxia Ma4Guangfu Jin5Zhibin Hu6Juncheng Dai7Hongbing Shen8Department of Epidemiology and Biostatistics School of Public Health Nanjing Medical University Nanjing 211166 ChinaDepartment of Epidemiology and Biostatistics School of Public Health Nanjing Medical University Nanjing 211166 ChinaDepartment of Epidemiology and Biostatistics School of Public Health Nanjing Medical University Nanjing 211166 ChinaDepartment of Epidemiology and Biostatistics School of Public Health Nanjing Medical University Nanjing 211166 ChinaDepartment of Epidemiology and Biostatistics School of Public Health Nanjing Medical University Nanjing 211166 ChinaDepartment of Epidemiology and Biostatistics School of Public Health Nanjing Medical University Nanjing 211166 ChinaDepartment of Epidemiology and Biostatistics School of Public Health Nanjing Medical University Nanjing 211166 ChinaDepartment of Epidemiology and Biostatistics School of Public Health Nanjing Medical University Nanjing 211166 ChinaDepartment of Epidemiology and Biostatistics School of Public Health Nanjing Medical University Nanjing 211166 ChinaAbstract Emerging evidence indicates that circular RNAs (circRNAs) are implicated in cancer development. This study aimed to evaluate whether circulating circRNAs may serve as novel biomarkers for non‐small cell lung cancer (NSCLC). We used RNA sequencing (RNA‐seq) and quantitative real‐time PCR to explore cancer‐related circRNAs. Bioinformatics and functional analyses were performed to reveal biological effects of circRNAs on lung cancer cells. A total of 5471 distinct circRNAs were identified by total RNA‐seq, in which 185 were differentially expressed between cancerous and adjacent normal tissues. A circRNA derived from exon 5–7 of the FARSA gene, termed circFARSA, was observed to increase in cancerous tissues (P = 0.016), and was more abundant in patients’ plasma than controls (P < 0.001). Overexpression of circFARSA in A549 cell line significantly promoted cell migration and invasion. In silico analysis suggested that circFARSA might sponge miR‐330‐5p and miR‐326, thereby relieving their inhibitory effects on oncogene fatty acid synthase. Summarily, this study revealed circRNA profile of NSCLC for the first time and provided the evidence of plasma circFARSA as a potential noninvasive biomarker for this malignancy.https://doi.org/10.1002/cam4.1514Circular RNAlung cancerplasmaRNA sequencing |
| spellingShingle | Dong Hang Jing Zhou Na Qin Wen Zhou Hongxia Ma Guangfu Jin Zhibin Hu Juncheng Dai Hongbing Shen A novel plasma circular RNA circFARSA is a potential biomarker for non‐small cell lung cancer Cancer Medicine Circular RNA lung cancer plasma RNA sequencing |
| title | A novel plasma circular RNA circFARSA is a potential biomarker for non‐small cell lung cancer |
| title_full | A novel plasma circular RNA circFARSA is a potential biomarker for non‐small cell lung cancer |
| title_fullStr | A novel plasma circular RNA circFARSA is a potential biomarker for non‐small cell lung cancer |
| title_full_unstemmed | A novel plasma circular RNA circFARSA is a potential biomarker for non‐small cell lung cancer |
| title_short | A novel plasma circular RNA circFARSA is a potential biomarker for non‐small cell lung cancer |
| title_sort | novel plasma circular rna circfarsa is a potential biomarker for non small cell lung cancer |
| topic | Circular RNA lung cancer plasma RNA sequencing |
| url | https://doi.org/10.1002/cam4.1514 |
| work_keys_str_mv | AT donghang anovelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT jingzhou anovelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT naqin anovelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT wenzhou anovelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT hongxiama anovelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT guangfujin anovelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT zhibinhu anovelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT junchengdai anovelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT hongbingshen anovelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT donghang novelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT jingzhou novelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT naqin novelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT wenzhou novelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT hongxiama novelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT guangfujin novelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT zhibinhu novelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT junchengdai novelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer AT hongbingshen novelplasmacircularrnacircfarsaisapotentialbiomarkerfornonsmallcelllungcancer |