HIF1α and SOX2 expression in cervical squamous cell carcinoma
Background/Aim. Hypoxia is one of the major changes that occurs in the tumor microenvironment. It has been observed that there are pluripotent cancer cells in the cancer cell population that affect tumor growth and their resistance to therapy. The aim of this study was to examine the expression of h...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2019-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2019/0042-84501800043M.pdf |
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| author | Milenković Svetlana V. Terzić Tatjana Vraneš Boris Soldatović Ivan |
| author_facet | Milenković Svetlana V. Terzić Tatjana Vraneš Boris Soldatović Ivan |
| author_sort | Milenković Svetlana V. |
| collection | DOAJ |
| description | Background/Aim. Hypoxia is one of the major changes that occurs in the tumor microenvironment. It has been observed that there are pluripotent cancer cells in the cancer cell population that affect tumor growth and their resistance to therapy. The aim of this study was to examine the expression of hypoxia-inducible factor-1 alpha (HIF-1α), endogenous marker of hypoxia, and SOX2, marker of the pluripotent stem cells existing in the normal adult tissues, in the cervical squamous cell carcinoma (SCC). Methods. The study was conducted in 90 women with invasive cervical SCC, divided into two groups – 60 women in the Group A, with FIGO IB1 < 20 mm tumors (no metastases in the lymph nodes), and 30 women in the group B with tumors FIGO I–II (positive lymph nodes). The basic clinical parameters were determined by standard histopathological analysis, and the expression of HIF-1α and SOX2 by immunohistochemical examination. Results. There was a significant difference between the groups A and B, in the expression of HIF-1α (p = 0.024), but not in the expression of SOX2 (p = 0.566). Expression of HIF-1α was significantly higher in the group with lymph node metastases and invasion of lymphovascular spaces (p <0.001) but not associated with tumor size (p = 0.291) or lymphocytic stromal response (p = 0.940). The tumor grade significantly influenced the expression of HIF-1α (p = 0.013). The expression of SOX2 did not significantly correlate with any of the established clinical tumor parameters. Conclusion. A significant difference in the expression of HIF-1 α between the group with and that without metastases in lymph nodes in invasive cervical SCC could distinguish HIF-1α as a parameter of poor prognosis of the disease. The prognostic significance of SOX2 as well as a significant correlation between expression of HIF-1α and SOX2 were not established. |
| format | Article |
| id | doaj-art-157a0d7f752d46829a8e39bafee44a6a |
| institution | OA Journals |
| issn | 0042-8450 2406-0720 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-157a0d7f752d46829a8e39bafee44a6a2025-08-20T01:58:41ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202019-01-0176121253126010.2298/VSP171213043M0042-84501800043MHIF1α and SOX2 expression in cervical squamous cell carcinomaMilenković Svetlana V.0Terzić Tatjana1Vraneš Boris2Soldatović Ivan3Clinical Center of Serbia, Pathology Department, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, Institute of Pathology, Belgrade, SerbiaClinical Center of Serbia, Clinic for Gynecology and Obstetrics, Belgrade, SerbiaInstitute for Medical Statistics and Informatics, Belgrade, SerbiaBackground/Aim. Hypoxia is one of the major changes that occurs in the tumor microenvironment. It has been observed that there are pluripotent cancer cells in the cancer cell population that affect tumor growth and their resistance to therapy. The aim of this study was to examine the expression of hypoxia-inducible factor-1 alpha (HIF-1α), endogenous marker of hypoxia, and SOX2, marker of the pluripotent stem cells existing in the normal adult tissues, in the cervical squamous cell carcinoma (SCC). Methods. The study was conducted in 90 women with invasive cervical SCC, divided into two groups – 60 women in the Group A, with FIGO IB1 < 20 mm tumors (no metastases in the lymph nodes), and 30 women in the group B with tumors FIGO I–II (positive lymph nodes). The basic clinical parameters were determined by standard histopathological analysis, and the expression of HIF-1α and SOX2 by immunohistochemical examination. Results. There was a significant difference between the groups A and B, in the expression of HIF-1α (p = 0.024), but not in the expression of SOX2 (p = 0.566). Expression of HIF-1α was significantly higher in the group with lymph node metastases and invasion of lymphovascular spaces (p <0.001) but not associated with tumor size (p = 0.291) or lymphocytic stromal response (p = 0.940). The tumor grade significantly influenced the expression of HIF-1α (p = 0.013). The expression of SOX2 did not significantly correlate with any of the established clinical tumor parameters. Conclusion. A significant difference in the expression of HIF-1 α between the group with and that without metastases in lymph nodes in invasive cervical SCC could distinguish HIF-1α as a parameter of poor prognosis of the disease. The prognostic significance of SOX2 as well as a significant correlation between expression of HIF-1α and SOX2 were not established.http://www.doiserbia.nb.rs/img/doi/0042-8450/2019/0042-84501800043M.pdfuterine cervical neoplasmsneoplasm stagingprognosishypoxiabiomarkers |
| spellingShingle | Milenković Svetlana V. Terzić Tatjana Vraneš Boris Soldatović Ivan HIF1α and SOX2 expression in cervical squamous cell carcinoma Vojnosanitetski Pregled uterine cervical neoplasms neoplasm staging prognosis hypoxia biomarkers |
| title | HIF1α and SOX2 expression in cervical squamous cell carcinoma |
| title_full | HIF1α and SOX2 expression in cervical squamous cell carcinoma |
| title_fullStr | HIF1α and SOX2 expression in cervical squamous cell carcinoma |
| title_full_unstemmed | HIF1α and SOX2 expression in cervical squamous cell carcinoma |
| title_short | HIF1α and SOX2 expression in cervical squamous cell carcinoma |
| title_sort | hif1α and sox2 expression in cervical squamous cell carcinoma |
| topic | uterine cervical neoplasms neoplasm staging prognosis hypoxia biomarkers |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2019/0042-84501800043M.pdf |
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