Analysis of lipidomics profile of Brassica napus hybrid ‘Fangyou 777’ and its parents during ripening stages based on UPLC-MS/MS
Abstract Background Lipids in rapeseed is of great significance to human health, and ‘Fangyou 777’ (No. GPD-2019-510073) has been identified as an excellent cultivar with high oil content. However, the change of lipid profile at different ripening stages remain unclear. Herein, UPLC-MS/MS was utiliz...
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2025-02-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-06220-7 |
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| author | Tao Zheng Jianmei Yang Qiao Chen Xinxin Huang Yan Xue Qi Tang Guodong Wang Ying Li Zhubing Hu Haitao T. Zeng |
| author_facet | Tao Zheng Jianmei Yang Qiao Chen Xinxin Huang Yan Xue Qi Tang Guodong Wang Ying Li Zhubing Hu Haitao T. Zeng |
| author_sort | Tao Zheng |
| collection | DOAJ |
| description | Abstract Background Lipids in rapeseed is of great significance to human health, and ‘Fangyou 777’ (No. GPD-2019-510073) has been identified as an excellent cultivar with high oil content. However, the change of lipid profile at different ripening stages remain unclear. Herein, UPLC-MS/MS was utilized for comprehensive lipidomics analysis of ‘Fangyou 777’ and its parents at four ripening stages. Results 778 lipids components across 25 subclasses were identified, and triglycerides (TGs), diglycerides (DGs), phosphatidylserines (PSs), phosphatidylinositols (PIs), phosphatidylglycerols (PGs), phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), and free fatty acids (FFAs) were identified as the dominant lipid subclass. Due to heterotic vigor, the total lipids, TGs, FFAs, lysophosphatidylglycerol (LPGs) and PSs contents in ‘Fangyou 777’ were significantly higher than its parents. The PCA and OPLS-DA results elucidated that lipids in ‘Fangyou 777’ differed obviously from its parents at S1 (17 April, 2023; 28 days before ripening, 28 DBR), S2 (1 May, 2023; 14 DBR), and S3 (15 May, 2023; ripening day). TG(18:1_18:3_22:1), TG(18:1_22:1_18:2), TG(16:0_18:1_20:1), TG(16:0_18:1_22:1), TG(20:1_18:2_20:2), TG(18:1_18:1_20:1), and FFA(24:4) were recognized as key differential lipids. The glycerolipid metabolism and unsaturated fatty acid biosynthesis were the differential metabolic pathways at S1 and S3, while glycosylphosphatidylinositol (GPI)-anchor biosynthesis and glycerophospholipid metabolism were the differential metabolic pathways at S2 and S4 (7 days after ripening/physiologically ripened for one week). Conclusion This study provided a comprehensive profile to facilitate the understanding lipids accumulation in ‘Fangyou 777’ and its parents during ripening stages, and offered a foundation to comprehend lipid metabolism. |
| format | Article |
| id | doaj-art-156852cd656641cc8a63ee919a1467a1 |
| institution | DOAJ |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-02-01 |
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| series | BMC Plant Biology |
| spelling | doaj-art-156852cd656641cc8a63ee919a1467a12025-08-20T02:48:30ZengBMCBMC Plant Biology1471-22292025-02-0125111310.1186/s12870-025-06220-7Analysis of lipidomics profile of Brassica napus hybrid ‘Fangyou 777’ and its parents during ripening stages based on UPLC-MS/MSTao Zheng0Jianmei Yang1Qiao Chen2Xinxin Huang3Yan Xue4Qi Tang5Guodong Wang6Ying Li7Zhubing Hu8Haitao T. Zeng9School of Biological Science and Engineering, Shaanxi University of TechnologySchool of Biological Science and Engineering, Shaanxi University of TechnologyHanzhong Vocational and Technical CollegeSchool of Biological Science and Engineering, Shaanxi University of TechnologyHanzhong Institute of Agricultural SciencesSchool of Biological Science and Engineering, Shaanxi University of TechnologyCollege of Life Sciences, Engineering Research Center of High Value Utilization of Western China Fruit Resources of Ministry of Education, Shaanxi Normal UniversityHanzhong Institute of Agricultural SciencesHenan UniversitySchool of Biological Science and Engineering, Shaanxi University of TechnologyAbstract Background Lipids in rapeseed is of great significance to human health, and ‘Fangyou 777’ (No. GPD-2019-510073) has been identified as an excellent cultivar with high oil content. However, the change of lipid profile at different ripening stages remain unclear. Herein, UPLC-MS/MS was utilized for comprehensive lipidomics analysis of ‘Fangyou 777’ and its parents at four ripening stages. Results 778 lipids components across 25 subclasses were identified, and triglycerides (TGs), diglycerides (DGs), phosphatidylserines (PSs), phosphatidylinositols (PIs), phosphatidylglycerols (PGs), phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), and free fatty acids (FFAs) were identified as the dominant lipid subclass. Due to heterotic vigor, the total lipids, TGs, FFAs, lysophosphatidylglycerol (LPGs) and PSs contents in ‘Fangyou 777’ were significantly higher than its parents. The PCA and OPLS-DA results elucidated that lipids in ‘Fangyou 777’ differed obviously from its parents at S1 (17 April, 2023; 28 days before ripening, 28 DBR), S2 (1 May, 2023; 14 DBR), and S3 (15 May, 2023; ripening day). TG(18:1_18:3_22:1), TG(18:1_22:1_18:2), TG(16:0_18:1_20:1), TG(16:0_18:1_22:1), TG(20:1_18:2_20:2), TG(18:1_18:1_20:1), and FFA(24:4) were recognized as key differential lipids. The glycerolipid metabolism and unsaturated fatty acid biosynthesis were the differential metabolic pathways at S1 and S3, while glycosylphosphatidylinositol (GPI)-anchor biosynthesis and glycerophospholipid metabolism were the differential metabolic pathways at S2 and S4 (7 days after ripening/physiologically ripened for one week). Conclusion This study provided a comprehensive profile to facilitate the understanding lipids accumulation in ‘Fangyou 777’ and its parents during ripening stages, and offered a foundation to comprehend lipid metabolism.https://doi.org/10.1186/s12870-025-06220-7Brassica napus‘Fangyou 777’UPLC-MS/MSLipids compositionRipening stages |
| spellingShingle | Tao Zheng Jianmei Yang Qiao Chen Xinxin Huang Yan Xue Qi Tang Guodong Wang Ying Li Zhubing Hu Haitao T. Zeng Analysis of lipidomics profile of Brassica napus hybrid ‘Fangyou 777’ and its parents during ripening stages based on UPLC-MS/MS BMC Plant Biology Brassica napus ‘Fangyou 777’ UPLC-MS/MS Lipids composition Ripening stages |
| title | Analysis of lipidomics profile of Brassica napus hybrid ‘Fangyou 777’ and its parents during ripening stages based on UPLC-MS/MS |
| title_full | Analysis of lipidomics profile of Brassica napus hybrid ‘Fangyou 777’ and its parents during ripening stages based on UPLC-MS/MS |
| title_fullStr | Analysis of lipidomics profile of Brassica napus hybrid ‘Fangyou 777’ and its parents during ripening stages based on UPLC-MS/MS |
| title_full_unstemmed | Analysis of lipidomics profile of Brassica napus hybrid ‘Fangyou 777’ and its parents during ripening stages based on UPLC-MS/MS |
| title_short | Analysis of lipidomics profile of Brassica napus hybrid ‘Fangyou 777’ and its parents during ripening stages based on UPLC-MS/MS |
| title_sort | analysis of lipidomics profile of brassica napus hybrid fangyou 777 and its parents during ripening stages based on uplc ms ms |
| topic | Brassica napus ‘Fangyou 777’ UPLC-MS/MS Lipids composition Ripening stages |
| url | https://doi.org/10.1186/s12870-025-06220-7 |
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