Potential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2
Abstract Background Periodontitis is a prevalent oral disease that significantly impacts human quality of life. This study aimed at the influence of microRNA-299-5p (miR-299-5p) on regulating lipopolysaccharide (LPS)-induced osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Met...
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2025-02-01
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| Online Access: | https://doi.org/10.1186/s12903-025-05617-y |
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| author | Qing-Hua Zhang Yu-Xi Zhang Ling Zhang |
| author_facet | Qing-Hua Zhang Yu-Xi Zhang Ling Zhang |
| author_sort | Qing-Hua Zhang |
| collection | DOAJ |
| description | Abstract Background Periodontitis is a prevalent oral disease that significantly impacts human quality of life. This study aimed at the influence of microRNA-299-5p (miR-299-5p) on regulating lipopolysaccharide (LPS)-induced osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Methods The 105 periodontitis patients and 102 healthy periodontal volunteers (HC) were recruited with their clinical baseline data. miR-299-5p expression in saliva was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). To identify the target relationship between miR-299-5p and pumilio RNA-binding family member 2 (PUM2), the dual luciferase reporter gene was explored. PDLSCs were treated with LPS and subjected to osteogenic differentiation induction. The secretion of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) was determined by enzyme-linked immunosorbent assay. mRNA expression of runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteocalcin (OCN) was detected by qRT-PCR. Results miR-299-5p expression was markedly reduced in periodontitis patients’ saliva and negatively correlated with patients’ attachment loss, bleeding index, plaque index, probing pocket depth, and TNF-α, IL-6, and IL-1β levels. miR-299-5p could be well distinguished between periodontitis and HC. LPS induction dramatically stimulated the secretion of inflammatory factors and inhibited the differentiation of PDLSCs, which was counteracted by miR-299-5p overexpression. miR-299-5p was detected to target PUM2, which could exacerbate the inflammatory condition of PDLSCs and lead to differentiation defects. Conclusions miR-299-5p has emerged as a promising diagnostic marker for periodontitis and may attenuate inflammation and contribute to osteogenic differentiation in PDLSCs cells by targeting PUM2. |
| format | Article |
| id | doaj-art-1564a95075474a2fa511e9386ecef31c |
| institution | DOAJ |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
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| series | BMC Oral Health |
| spelling | doaj-art-1564a95075474a2fa511e9386ecef31c2025-08-20T03:10:49ZengBMCBMC Oral Health1472-68312025-02-0125111110.1186/s12903-025-05617-yPotential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2Qing-Hua Zhang0Yu-Xi Zhang1Ling Zhang2Department of Orthodontics, Shanxi Provincial People’s HospitalUniversity of California BerkeleyDepartment of Oralsurgery, Shanxi Provincial People’s HospitalAbstract Background Periodontitis is a prevalent oral disease that significantly impacts human quality of life. This study aimed at the influence of microRNA-299-5p (miR-299-5p) on regulating lipopolysaccharide (LPS)-induced osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Methods The 105 periodontitis patients and 102 healthy periodontal volunteers (HC) were recruited with their clinical baseline data. miR-299-5p expression in saliva was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). To identify the target relationship between miR-299-5p and pumilio RNA-binding family member 2 (PUM2), the dual luciferase reporter gene was explored. PDLSCs were treated with LPS and subjected to osteogenic differentiation induction. The secretion of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) was determined by enzyme-linked immunosorbent assay. mRNA expression of runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteocalcin (OCN) was detected by qRT-PCR. Results miR-299-5p expression was markedly reduced in periodontitis patients’ saliva and negatively correlated with patients’ attachment loss, bleeding index, plaque index, probing pocket depth, and TNF-α, IL-6, and IL-1β levels. miR-299-5p could be well distinguished between periodontitis and HC. LPS induction dramatically stimulated the secretion of inflammatory factors and inhibited the differentiation of PDLSCs, which was counteracted by miR-299-5p overexpression. miR-299-5p was detected to target PUM2, which could exacerbate the inflammatory condition of PDLSCs and lead to differentiation defects. Conclusions miR-299-5p has emerged as a promising diagnostic marker for periodontitis and may attenuate inflammation and contribute to osteogenic differentiation in PDLSCs cells by targeting PUM2.https://doi.org/10.1186/s12903-025-05617-yChronic periodontitisPDLSCsInflammatoryDifferentiationimiR-299-5pPUM2 |
| spellingShingle | Qing-Hua Zhang Yu-Xi Zhang Ling Zhang Potential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2 BMC Oral Health Chronic periodontitis PDLSCs Inflammatory Differentiation imiR-299-5p PUM2 |
| title | Potential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2 |
| title_full | Potential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2 |
| title_fullStr | Potential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2 |
| title_full_unstemmed | Potential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2 |
| title_short | Potential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2 |
| title_sort | potential of mir 299 5p to modulate lps induced inflammation and osteogenic differentiation of periodontal stem cells by targeting pum2 |
| topic | Chronic periodontitis PDLSCs Inflammatory Differentiation imiR-299-5p PUM2 |
| url | https://doi.org/10.1186/s12903-025-05617-y |
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