Cytauxzoon felis in Domestic Cats: A Molecular Study Using Real-Time PCR in Semnan, Iran
Background: Cytauxzoon felis is a protozoan parasite transmitted by ticks and affects felids. Acute infection in domestic cats is characterized by symptoms such as lethargy, anorexia, fever, and anemia. Methods: The present study focuses on diagnosing and molecularly identifying C. felis using a re...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2025-06-01
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| Series: | Iranian Journal of Parasitology |
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| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/4470 |
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| author | Masoud Ebrahimi Toodarvari Hesamedin Eskafian Hamid Staji Mahmood Ahmadi-hamedni |
| author_facet | Masoud Ebrahimi Toodarvari Hesamedin Eskafian Hamid Staji Mahmood Ahmadi-hamedni |
| author_sort | Masoud Ebrahimi Toodarvari |
| collection | DOAJ |
| description |
Background: Cytauxzoon felis is a protozoan parasite transmitted by ticks and affects felids. Acute infection in domestic cats is characterized by symptoms such as lethargy, anorexia, fever, and anemia.
Methods: The present study focuses on diagnosing and molecularly identifying C. felis using a real-time PCR method in cats from Semnan, Iran. During the winter and spring of 2024, two hundred cats were randomly selected from veterinary clinics in Semnan. Blood samples were collected from the cats for DNA extraction and molecular analysis. Samples were divided into 40 pooled of 5 samples, each consisting of a combination of 5 blood samples. Then, the genomic DNAs were extracted from blood specimens and screened by real-time PCR (RT-PCR) for the presence of C. felis infection by amplifying of ITS2 gene belonging to the Cytauxzoon genus.
Results: The results indicated that 6 out of the 200 blood samples were infected (3%).
Conclusion: This study was conducted for the first time in Semnan and shows that the prevalence of C. felis in cats is significant.
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| format | Article |
| id | doaj-art-155cc4f85ea041b499858362db59e45f |
| institution | DOAJ |
| issn | 1735-7020 2008-238X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj-art-155cc4f85ea041b499858362db59e45f2025-08-20T03:16:56ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2025-06-0120210.18502/ijpa.v20i2.19033Cytauxzoon felis in Domestic Cats: A Molecular Study Using Real-Time PCR in Semnan, IranMasoud Ebrahimi Toodarvari0Hesamedin Eskafian1Hamid Staji2Mahmood Ahmadi-hamedni3Department of Veterinary Medicine, Faculty of Veterinary Medicine, Semnan University, Semnan, IranDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, IranDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran Background: Cytauxzoon felis is a protozoan parasite transmitted by ticks and affects felids. Acute infection in domestic cats is characterized by symptoms such as lethargy, anorexia, fever, and anemia. Methods: The present study focuses on diagnosing and molecularly identifying C. felis using a real-time PCR method in cats from Semnan, Iran. During the winter and spring of 2024, two hundred cats were randomly selected from veterinary clinics in Semnan. Blood samples were collected from the cats for DNA extraction and molecular analysis. Samples were divided into 40 pooled of 5 samples, each consisting of a combination of 5 blood samples. Then, the genomic DNAs were extracted from blood specimens and screened by real-time PCR (RT-PCR) for the presence of C. felis infection by amplifying of ITS2 gene belonging to the Cytauxzoon genus. Results: The results indicated that 6 out of the 200 blood samples were infected (3%). Conclusion: This study was conducted for the first time in Semnan and shows that the prevalence of C. felis in cats is significant. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/4470Cytauxzoon felisFelidsIranReal-time PCR |
| spellingShingle | Masoud Ebrahimi Toodarvari Hesamedin Eskafian Hamid Staji Mahmood Ahmadi-hamedni Cytauxzoon felis in Domestic Cats: A Molecular Study Using Real-Time PCR in Semnan, Iran Iranian Journal of Parasitology Cytauxzoon felis Felids Iran Real-time PCR |
| title | Cytauxzoon felis in Domestic Cats: A Molecular Study Using Real-Time PCR in Semnan, Iran |
| title_full | Cytauxzoon felis in Domestic Cats: A Molecular Study Using Real-Time PCR in Semnan, Iran |
| title_fullStr | Cytauxzoon felis in Domestic Cats: A Molecular Study Using Real-Time PCR in Semnan, Iran |
| title_full_unstemmed | Cytauxzoon felis in Domestic Cats: A Molecular Study Using Real-Time PCR in Semnan, Iran |
| title_short | Cytauxzoon felis in Domestic Cats: A Molecular Study Using Real-Time PCR in Semnan, Iran |
| title_sort | cytauxzoon felis in domestic cats a molecular study using real time pcr in semnan iran |
| topic | Cytauxzoon felis Felids Iran Real-time PCR |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/4470 |
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