STITCHER: A web resource for high-throughput design of primers for overlapping PCR applications
Overlapping PCR is routinely used in a wide number of molecular applications. These include stitching PCR fragments together, generating fluorescent transcriptional and translational fusions, inserting mutations, making deletions, and PCR cloning. Overlapping PCR is also used for genotyping by tradi...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2015-06-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000114301 |
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