Genomic evidence of active circulation of Orthobunyavirus in Ecuador

Background: Between 2023 and 2025, the largest Oropouche fever epidemic recorded in history unfolded across Brazil seeding cases throughout Latin America. In 2024, three cases of Oropouche fever were reported in Ecuador. Methods: An Oropouche fever case detected in Bolívar Province in June 2024 was...

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Main Authors: Daniel Romero-Alvarez, Juan Carlos Navarro, Diana Gutiérrez-Pallo, Gabriela Echeverría-Garcés, Camila Lara-Cazorla, Byron Fuertes-Flores, Génesis García-Díaz, Gabriel da Luz Wallau, Josefina Coloma, Andrés Carrazco-Montalvo
Format: Article
Language:English
Published: Elsevier 2025-10-01
Series:International Journal of Infectious Diseases
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Online Access:http://www.sciencedirect.com/science/article/pii/S1201971225002243
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author Daniel Romero-Alvarez
Juan Carlos Navarro
Diana Gutiérrez-Pallo
Gabriela Echeverría-Garcés
Camila Lara-Cazorla
Byron Fuertes-Flores
Génesis García-Díaz
Gabriel da Luz Wallau
Josefina Coloma
Andrés Carrazco-Montalvo
author_facet Daniel Romero-Alvarez
Juan Carlos Navarro
Diana Gutiérrez-Pallo
Gabriela Echeverría-Garcés
Camila Lara-Cazorla
Byron Fuertes-Flores
Génesis García-Díaz
Gabriel da Luz Wallau
Josefina Coloma
Andrés Carrazco-Montalvo
author_sort Daniel Romero-Alvarez
collection DOAJ
description Background: Between 2023 and 2025, the largest Oropouche fever epidemic recorded in history unfolded across Brazil seeding cases throughout Latin America. In 2024, three cases of Oropouche fever were reported in Ecuador. Methods: An Oropouche fever case detected in Bolívar Province in June 2024 was preliminarily diagnosed as Oropouche virus (OROV) through RT-qPCR and was further processed using next-generation sequencing. Results: Segments L and S of this virus forms a monophyly with another sequence circulating in Ecuador in April 2024 (i.e. PQ863772.1 isolate Ecuador traveler), with an uncertain province origin. Both sequences differ from previous OROV Ecuadorian sequences detected in 2016 and from the OROV strain driving the 2023-2025 epidemic in Brazil. Conclusions: Orthobunyavirus oropoucheense has an endemic circulation in Ecuador. Genomic surveillance of Orthobunyavirus in Ecuador and other regions should be actively pursued—independent of epidemics—to anticipate potential zoonotic outbreaks.
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spelling doaj-art-1557e95934b849959e4cc0fa39b9c7d82025-08-22T04:55:48ZengElsevierInternational Journal of Infectious Diseases1201-97122025-10-0115910800010.1016/j.ijid.2025.108000Genomic evidence of active circulation of Orthobunyavirus in EcuadorDaniel Romero-Alvarez0Juan Carlos Navarro1Diana Gutiérrez-Pallo2Gabriela Echeverría-Garcés3Camila Lara-Cazorla4Byron Fuertes-Flores5Génesis García-Díaz6Gabriel da Luz Wallau7Josefina Coloma8Andrés Carrazco-Montalvo9Research Group of Emerging and Neglected Diseases, Ecoepidemiology and Biodiversity, Health Science Faculty, School of Biomedical Sciences, Universidad Internacional SEK (UISEK), Quito, Ecuador; Instituto Nacional de Biodiversidad (INABIO), Quito, EcuadorResearch Group of Emerging and Neglected Diseases, Ecoepidemiology and Biodiversity, Health Science Faculty, School of Biomedical Sciences, Universidad Internacional SEK (UISEK), Quito, EcuadorCentro de Referencia Nacional de Genómica, Secuenciación y Bioinformática, Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez,” Quito, EcuadorCentro de Referencia Nacional de Genómica, Secuenciación y Bioinformática, Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez,” Quito, Ecuador; Experimental and Applied Biomedicine Research Group, Health Sciences Faculty, Universidad Internacional SEK (UISEK), Quito, EcuadorCentro de Referencia Nacional de Genómica, Secuenciación y Bioinformática, Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez,” Quito, EcuadorCentro de Referencia Nacional de Genómica, Secuenciación y Bioinformática, Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez,” Quito, EcuadorCentro de Referencia Nacional de Genómica, Secuenciación y Bioinformática, Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez,” Quito, EcuadorDepartamento de Entomologia, Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (Fiocruz), Recife, Brazil; Núcleo de Bioinformática, Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (Fiocruz/PE), Recife, Brazil; Department of Arbovirology and Entomology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; Universidade Federal de Santa Maria (UFSM), Santa Maria, BrazilDivision of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, and Sustainable Sciences Institute, Oakland, USACentro de Referencia Nacional de Genómica, Secuenciación y Bioinformática, Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez,” Quito, Ecuador; Corresponding author:Background: Between 2023 and 2025, the largest Oropouche fever epidemic recorded in history unfolded across Brazil seeding cases throughout Latin America. In 2024, three cases of Oropouche fever were reported in Ecuador. Methods: An Oropouche fever case detected in Bolívar Province in June 2024 was preliminarily diagnosed as Oropouche virus (OROV) through RT-qPCR and was further processed using next-generation sequencing. Results: Segments L and S of this virus forms a monophyly with another sequence circulating in Ecuador in April 2024 (i.e. PQ863772.1 isolate Ecuador traveler), with an uncertain province origin. Both sequences differ from previous OROV Ecuadorian sequences detected in 2016 and from the OROV strain driving the 2023-2025 epidemic in Brazil. Conclusions: Orthobunyavirus oropoucheense has an endemic circulation in Ecuador. Genomic surveillance of Orthobunyavirus in Ecuador and other regions should be actively pursued—independent of epidemics—to anticipate potential zoonotic outbreaks.http://www.sciencedirect.com/science/article/pii/S1201971225002243OrthobunyavirusOropouche virusOROVGenomic surveillanceEcuador
spellingShingle Daniel Romero-Alvarez
Juan Carlos Navarro
Diana Gutiérrez-Pallo
Gabriela Echeverría-Garcés
Camila Lara-Cazorla
Byron Fuertes-Flores
Génesis García-Díaz
Gabriel da Luz Wallau
Josefina Coloma
Andrés Carrazco-Montalvo
Genomic evidence of active circulation of Orthobunyavirus in Ecuador
International Journal of Infectious Diseases
Orthobunyavirus
Oropouche virus
OROV
Genomic surveillance
Ecuador
title Genomic evidence of active circulation of Orthobunyavirus in Ecuador
title_full Genomic evidence of active circulation of Orthobunyavirus in Ecuador
title_fullStr Genomic evidence of active circulation of Orthobunyavirus in Ecuador
title_full_unstemmed Genomic evidence of active circulation of Orthobunyavirus in Ecuador
title_short Genomic evidence of active circulation of Orthobunyavirus in Ecuador
title_sort genomic evidence of active circulation of orthobunyavirus in ecuador
topic Orthobunyavirus
Oropouche virus
OROV
Genomic surveillance
Ecuador
url http://www.sciencedirect.com/science/article/pii/S1201971225002243
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