Baicalein based nano-delivery system restores mitochondrial homeostasis through PPAR signaling pathway to promote wound healing in diabetes
Abstract Wound healing in diabetes is a substantial clinical challenge due to the hyperglycemic microenvironment, high pH, bacterial infection, persistent inflammation, and impaired cellular functions, attributed to mitochondrial dysfunction. Here, we have developed an injectable photo-crosslinking...
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BMC
2025-05-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03427-6 |
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| author | Danlei Qin Weiting Hu Yanqin Guo Rui Cheng Fengxiang Hao Bin Zhao |
| author_facet | Danlei Qin Weiting Hu Yanqin Guo Rui Cheng Fengxiang Hao Bin Zhao |
| author_sort | Danlei Qin |
| collection | DOAJ |
| description | Abstract Wound healing in diabetes is a substantial clinical challenge due to the hyperglycemic microenvironment, high pH, bacterial infection, persistent inflammation, and impaired cellular functions, attributed to mitochondrial dysfunction. Here, we have developed an injectable photo-crosslinking nanocomposite hydrogel (BA/GOx@ZIF-8@GelMA, BGZ@GelMA) with baicalein (BA) and glucose oxidase (GOx) loaded Zinc metal-organic framework (ZIF-8) based on methacrylated gelatin (GelMA) to accelerate diabetic infected wound healing by regulating subcellular and cellular functions. The combination of ZIF-8 and BA gives the hydrogel excellent antibacterial properties. A high blood sugar environment triggers the release of GOx in BGZ@GelMA, reducing local glucose and pH, producing hydrogen peroxide (H2O2), and releasing BA and Zinc ions (Zn2+). This process provides a suitable microenvironment for wound healing. Zn2+ can significantly inhibit the proliferation of Staphylococcus aureus (S.aureus) and Escherichia coli (E.coli). The released BA can clear ROS in cells and mitochondria, restore mitochondrial function and stability, and make the hydrogel fundamentally improve the cell function damage induced by hyperglycemia, and ultimately promote cell proliferation, migration and angiogenesis. In general, our multifunctional nanocomposite hydrogel provides a new strategy for diabetes wound healing at the subcellular and cellular functional levels. Graphical abstract |
| format | Article |
| id | doaj-art-154d950a6f3f421799005d088c9c8088 |
| institution | DOAJ |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-154d950a6f3f421799005d088c9c80882025-08-20T03:16:32ZengBMCJournal of Nanobiotechnology1477-31552025-05-0123112110.1186/s12951-025-03427-6Baicalein based nano-delivery system restores mitochondrial homeostasis through PPAR signaling pathway to promote wound healing in diabetesDanlei Qin0Weiting Hu1Yanqin Guo2Rui Cheng3Fengxiang Hao4Bin Zhao5Shanxi Medical University School and Hospital of Stomatology, Shanxi Province Key Laboratory of Oral Diseases Prevention and New MaterialsThe Second Clinical Medical College, Shanxi Medical UniversityDepartment of Ultrasound, The Second Hospital of Shanxi Medical UniversityDepartment of Endocrinology, The Second Hospital of Shanxi Medical UniversityShanxi Medical University School and Hospital of Stomatology, Shanxi Province Key Laboratory of Oral Diseases Prevention and New MaterialsShanxi Medical University School and Hospital of Stomatology, Shanxi Province Key Laboratory of Oral Diseases Prevention and New MaterialsAbstract Wound healing in diabetes is a substantial clinical challenge due to the hyperglycemic microenvironment, high pH, bacterial infection, persistent inflammation, and impaired cellular functions, attributed to mitochondrial dysfunction. Here, we have developed an injectable photo-crosslinking nanocomposite hydrogel (BA/GOx@ZIF-8@GelMA, BGZ@GelMA) with baicalein (BA) and glucose oxidase (GOx) loaded Zinc metal-organic framework (ZIF-8) based on methacrylated gelatin (GelMA) to accelerate diabetic infected wound healing by regulating subcellular and cellular functions. The combination of ZIF-8 and BA gives the hydrogel excellent antibacterial properties. A high blood sugar environment triggers the release of GOx in BGZ@GelMA, reducing local glucose and pH, producing hydrogen peroxide (H2O2), and releasing BA and Zinc ions (Zn2+). This process provides a suitable microenvironment for wound healing. Zn2+ can significantly inhibit the proliferation of Staphylococcus aureus (S.aureus) and Escherichia coli (E.coli). The released BA can clear ROS in cells and mitochondria, restore mitochondrial function and stability, and make the hydrogel fundamentally improve the cell function damage induced by hyperglycemia, and ultimately promote cell proliferation, migration and angiogenesis. In general, our multifunctional nanocomposite hydrogel provides a new strategy for diabetes wound healing at the subcellular and cellular functional levels. Graphical abstracthttps://doi.org/10.1186/s12951-025-03427-6BaicaleinMetal-organic frameworkMitochondrionDiabetic wound healing |
| spellingShingle | Danlei Qin Weiting Hu Yanqin Guo Rui Cheng Fengxiang Hao Bin Zhao Baicalein based nano-delivery system restores mitochondrial homeostasis through PPAR signaling pathway to promote wound healing in diabetes Journal of Nanobiotechnology Baicalein Metal-organic framework Mitochondrion Diabetic wound healing |
| title | Baicalein based nano-delivery system restores mitochondrial homeostasis through PPAR signaling pathway to promote wound healing in diabetes |
| title_full | Baicalein based nano-delivery system restores mitochondrial homeostasis through PPAR signaling pathway to promote wound healing in diabetes |
| title_fullStr | Baicalein based nano-delivery system restores mitochondrial homeostasis through PPAR signaling pathway to promote wound healing in diabetes |
| title_full_unstemmed | Baicalein based nano-delivery system restores mitochondrial homeostasis through PPAR signaling pathway to promote wound healing in diabetes |
| title_short | Baicalein based nano-delivery system restores mitochondrial homeostasis through PPAR signaling pathway to promote wound healing in diabetes |
| title_sort | baicalein based nano delivery system restores mitochondrial homeostasis through ppar signaling pathway to promote wound healing in diabetes |
| topic | Baicalein Metal-organic framework Mitochondrion Diabetic wound healing |
| url | https://doi.org/10.1186/s12951-025-03427-6 |
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