Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali
Introduction: The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL frequency in Enterobacteriaceae isolated from patients’ blood cultures in two university teaching hospitals...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2016-10-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/7536 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850036280320065536 |
|---|---|
| author | Samba Adama Sangare Almoustapha Issiaka Maiga Ibrehima Guindo Aminata Maiga Namory Camara Oumar Agaly Dicko Souleymane Diallo Flabou Bougoudogo Laurence Armand-Lefevre Antoine Andremont Ibrahim Izetiegouma Maiga |
| author_facet | Samba Adama Sangare Almoustapha Issiaka Maiga Ibrehima Guindo Aminata Maiga Namory Camara Oumar Agaly Dicko Souleymane Diallo Flabou Bougoudogo Laurence Armand-Lefevre Antoine Andremont Ibrahim Izetiegouma Maiga |
| author_sort | Samba Adama Sangare |
| collection | DOAJ |
| description | Introduction: The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL frequency in Enterobacteriaceae isolated from patients’ blood cultures in two university teaching hospitals of Bamako, Mali.
Methodology: During a three-month period, the presence of Enterobacteriaceae from blood cultures of patients admitted to the university teaching hospitals of Bamako was evaluated. The microbial identifications were initially performed with an API 20E gallery and VITEK2 locally in Mali, and then confirmation in France was performed with a mass spectrometry MALDI-TOF in the bacteriology laboratory of the university teaching hospital of Bichat. Antibiotic susceptibility profiles were determined by the diffusion method as recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST).
Results: The isolated species were K. pneumoniae (14/40; 35.0%), E. coli (11/40; 27.5%), and E. cloacae (9/40; 22.5%). Of the strains isolated, 21/34 (61.8%) had an ESBL phenotype, including 10/14 (71.4%) K. pneumoniae, 8/11 (72.7%) E. coli, and 3/9 (33.3%) E. cloacae.
Resistances associated with ESBL strains of K. pneumoniae, E. coli, and E. cloacae were as follows: gentamicin (10/10, 100%; 6/8, 75%; 2/3, 67%, respectively), amikacin (2/10, 20%; 0/8, 0%; 0/3, 0%, respectively), ofloxacin (8/10, 80%; 7/8, 87%; 3/3, 100%, respectively), and cotrimoxazole (10/10, 100%; 6/8, 75%; 3/3, 100%, respectively).
Conclusion: Almost two-thirds (61.8%) of Enterobacteriaceae isolated from our blood cultures were ESBL producers. Only susceptibilities to carbapenems and to amikacin were fully conserved within the strains.
|
| format | Article |
| id | doaj-art-154afaf1a2db4e1f9071bf5680d304be |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2016-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-154afaf1a2db4e1f9071bf5680d304be2025-08-20T02:57:13ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802016-10-01101010.3855/jidc.7536Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in MaliSamba Adama Sangare0Almoustapha Issiaka Maiga1Ibrehima Guindo2Aminata Maiga3Namory Camara4Oumar Agaly Dicko5Souleymane Diallo6Flabou Bougoudogo7Laurence Armand-Lefevre8Antoine Andremont9Ibrahim Izetiegouma Maiga10Gabriel Touré University Teaching Hospital, Bamako, MaliGabriel Touré University Teaching Hospital, Bamako, MaliFaculty of Pharmacy, University of Sciences, Techniques, and Technologies of Bamako (USTTB), Bamako, MaliPoint G University Teaching Hospital, Bamako, MaliGabriel Touré University Teaching Hospital, Bamako, MaliPoint G University Teaching Hospital, Bamako, MaliFaculty of Pharmacy, University of Sciences, Techniques, and Technologies of Bamako (USTTB), Bamako, MaliFaculty of Pharmacy, University of Sciences, Techniques, and Technologies of Bamako (USTTB), Bamako, MaliBichat- Claude Bernard University Teaching Hospital and UMR INSERM 1137 Iame Paris, FranceBichat- Claude Bernard University Teaching Hospital and UMR INSERM 1137 Iame Paris, FrancePoint G University Teaching Hospital, Bamako, MaliIntroduction: The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL frequency in Enterobacteriaceae isolated from patients’ blood cultures in two university teaching hospitals of Bamako, Mali. Methodology: During a three-month period, the presence of Enterobacteriaceae from blood cultures of patients admitted to the university teaching hospitals of Bamako was evaluated. The microbial identifications were initially performed with an API 20E gallery and VITEK2 locally in Mali, and then confirmation in France was performed with a mass spectrometry MALDI-TOF in the bacteriology laboratory of the university teaching hospital of Bichat. Antibiotic susceptibility profiles were determined by the diffusion method as recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Results: The isolated species were K. pneumoniae (14/40; 35.0%), E. coli (11/40; 27.5%), and E. cloacae (9/40; 22.5%). Of the strains isolated, 21/34 (61.8%) had an ESBL phenotype, including 10/14 (71.4%) K. pneumoniae, 8/11 (72.7%) E. coli, and 3/9 (33.3%) E. cloacae. Resistances associated with ESBL strains of K. pneumoniae, E. coli, and E. cloacae were as follows: gentamicin (10/10, 100%; 6/8, 75%; 2/3, 67%, respectively), amikacin (2/10, 20%; 0/8, 0%; 0/3, 0%, respectively), ofloxacin (8/10, 80%; 7/8, 87%; 3/3, 100%, respectively), and cotrimoxazole (10/10, 100%; 6/8, 75%; 3/3, 100%, respectively). Conclusion: Almost two-thirds (61.8%) of Enterobacteriaceae isolated from our blood cultures were ESBL producers. Only susceptibilities to carbapenems and to amikacin were fully conserved within the strains. https://jidc.org/index.php/journal/article/view/7536Enterobacteriaceaeextended-spectrum beta-lactamasesblood culturesBamakoMali |
| spellingShingle | Samba Adama Sangare Almoustapha Issiaka Maiga Ibrehima Guindo Aminata Maiga Namory Camara Oumar Agaly Dicko Souleymane Diallo Flabou Bougoudogo Laurence Armand-Lefevre Antoine Andremont Ibrahim Izetiegouma Maiga Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali Journal of Infection in Developing Countries Enterobacteriaceae extended-spectrum beta-lactamases blood cultures Bamako Mali |
| title | Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali |
| title_full | Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali |
| title_fullStr | Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali |
| title_full_unstemmed | Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali |
| title_short | Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali |
| title_sort | prevalence of esbl producing enterobacteriaceae isolated from blood cultures in mali |
| topic | Enterobacteriaceae extended-spectrum beta-lactamases blood cultures Bamako Mali |
| url | https://jidc.org/index.php/journal/article/view/7536 |
| work_keys_str_mv | AT sambaadamasangare prevalenceofesblproducingenterobacteriaceaeisolatedfrombloodculturesinmali AT almoustaphaissiakamaiga prevalenceofesblproducingenterobacteriaceaeisolatedfrombloodculturesinmali AT ibrehimaguindo prevalenceofesblproducingenterobacteriaceaeisolatedfrombloodculturesinmali AT aminatamaiga prevalenceofesblproducingenterobacteriaceaeisolatedfrombloodculturesinmali AT namorycamara prevalenceofesblproducingenterobacteriaceaeisolatedfrombloodculturesinmali AT oumaragalydicko prevalenceofesblproducingenterobacteriaceaeisolatedfrombloodculturesinmali AT souleymanediallo prevalenceofesblproducingenterobacteriaceaeisolatedfrombloodculturesinmali AT flaboubougoudogo prevalenceofesblproducingenterobacteriaceaeisolatedfrombloodculturesinmali AT laurencearmandlefevre prevalenceofesblproducingenterobacteriaceaeisolatedfrombloodculturesinmali AT antoineandremont prevalenceofesblproducingenterobacteriaceaeisolatedfrombloodculturesinmali AT ibrahimizetiegoumamaiga prevalenceofesblproducingenterobacteriaceaeisolatedfrombloodculturesinmali |