Serum levels of Wnt5a in Egyptian women with obesity and their association with toll like receptor 2 Arg753Gln gene polymorphism in a pilot case control study of obesity as a state of metaflammation
Abstract Female obesity is a worldwide health issue linked to chronic metabolic low-grade inflammation (metaflammation) causing multiple obesity-related co-morbid conditions. We aimed to assess the serum levels of wingless integration site family member 5 A (Wnt5a), leptin, and tumor necrosis factor...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-85470-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585756941484032 |
|---|---|
| author | Shaimaa H. Fouad Mai Eldeeb Shereen A. Baioumy Sara I. Taha Rehab Ali Ibrahim Aya Elgendy Azza Omran Marwa Hamdy Raed A. Alharbi Abdulmajeed A. A. Sindi Sylvia W. Roman |
| author_facet | Shaimaa H. Fouad Mai Eldeeb Shereen A. Baioumy Sara I. Taha Rehab Ali Ibrahim Aya Elgendy Azza Omran Marwa Hamdy Raed A. Alharbi Abdulmajeed A. A. Sindi Sylvia W. Roman |
| author_sort | Shaimaa H. Fouad |
| collection | DOAJ |
| description | Abstract Female obesity is a worldwide health issue linked to chronic metabolic low-grade inflammation (metaflammation) causing multiple obesity-related co-morbid conditions. We aimed to assess the serum levels of wingless integration site family member 5 A (Wnt5a), leptin, and tumor necrosis factor-alpha (TNF-α) as markers of obesity-associated metaflammation and investigate the association with toll-like receptors2 (TLR2) gene (Arg753Gln) single nucleotide polymorphism (SNP) among Egyptian females. The study included 60 females with obesity and 30 matched controls. Serum levels of Wnt5a, leptin, and TNF-α were assessed by ELISA, while TLR2 (Arg753Gln) genotyping was done by PCR-RFLP. Serum Wnt5a, leptin, and TNF-α showed significantly higher levels in females with obesity than controls and a significant increase with higher classes of obesity. They showed significant positive correlations with each other. Only TNF-α and leptin were associated with metabolic syndrome (MetS) among the obesity group. According to TLR2 (Arg753Gln) SNP, the homozygous GG genotype was associated with elevated levels of Wnt5a, leptin, and TNF-α compared to the AA + GA model carriers. No significant differences were found in the distribution of TLR2 Arg753Gln (rs5743708) genotypes and alleles according to obesity or MetS, and the regression analysis showed no significant risk association. Serum Wnt5a, leptin, and TNF-α levels increase in women with obesity and the A allele of TLR2 (Arg753Gln) SNP could be protective against obesity-associated metaflammation. |
| format | Article |
| id | doaj-art-15474ec228c347b2a61315d137906e91 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-15474ec228c347b2a61315d137906e912025-01-26T12:29:44ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-025-85470-9Serum levels of Wnt5a in Egyptian women with obesity and their association with toll like receptor 2 Arg753Gln gene polymorphism in a pilot case control study of obesity as a state of metaflammationShaimaa H. Fouad0Mai Eldeeb1Shereen A. Baioumy2Sara I. Taha3Rehab Ali Ibrahim4Aya Elgendy5Azza Omran6Marwa Hamdy7Raed A. Alharbi8Abdulmajeed A. A. Sindi9Sylvia W. Roman10Department of Internal Medicine /Allergy and Clinical Immunology, Ain- Shams UniversityDepartment of Internal Medicine /Allergy and Clinical Immunology, Ain- Shams UniversityDepartment of Microbiology and Immunology, Faculty of Medicine, Zagazig UniversityDepartment of Clinical Pathology, Faculty of Medicine, Ain-Shams UniversityDepartment of Physical Medicine/Rheumatology and Rehabilitation, Faculty of Medicine, Ain-Shams UniversityDepartment of Internal Medicine /Allergy and Clinical Immunology, Ain- Shams UniversityDepartment of Cardiology, Faculty of Medicine, Ain-Shams UniversityDepartment of Medical Biochemistry and Molecular Biology, Ain-Shams UniversityDepartment of Laboratory Medicine, Faculty of Applied Medical Sciences, Al-Baha UniversityDepartment of Basic Medical Sciences, Faculty of Applied Medical Sciences, Al-Baha UniversityDepartment of Internal Medicine /Allergy and Clinical Immunology, Ain- Shams UniversityAbstract Female obesity is a worldwide health issue linked to chronic metabolic low-grade inflammation (metaflammation) causing multiple obesity-related co-morbid conditions. We aimed to assess the serum levels of wingless integration site family member 5 A (Wnt5a), leptin, and tumor necrosis factor-alpha (TNF-α) as markers of obesity-associated metaflammation and investigate the association with toll-like receptors2 (TLR2) gene (Arg753Gln) single nucleotide polymorphism (SNP) among Egyptian females. The study included 60 females with obesity and 30 matched controls. Serum levels of Wnt5a, leptin, and TNF-α were assessed by ELISA, while TLR2 (Arg753Gln) genotyping was done by PCR-RFLP. Serum Wnt5a, leptin, and TNF-α showed significantly higher levels in females with obesity than controls and a significant increase with higher classes of obesity. They showed significant positive correlations with each other. Only TNF-α and leptin were associated with metabolic syndrome (MetS) among the obesity group. According to TLR2 (Arg753Gln) SNP, the homozygous GG genotype was associated with elevated levels of Wnt5a, leptin, and TNF-α compared to the AA + GA model carriers. No significant differences were found in the distribution of TLR2 Arg753Gln (rs5743708) genotypes and alleles according to obesity or MetS, and the regression analysis showed no significant risk association. Serum Wnt5a, leptin, and TNF-α levels increase in women with obesity and the A allele of TLR2 (Arg753Gln) SNP could be protective against obesity-associated metaflammation.https://doi.org/10.1038/s41598-025-85470-9InflammationLeptinMetabolic syndromeObesityTLR2TNF-α |
| spellingShingle | Shaimaa H. Fouad Mai Eldeeb Shereen A. Baioumy Sara I. Taha Rehab Ali Ibrahim Aya Elgendy Azza Omran Marwa Hamdy Raed A. Alharbi Abdulmajeed A. A. Sindi Sylvia W. Roman Serum levels of Wnt5a in Egyptian women with obesity and their association with toll like receptor 2 Arg753Gln gene polymorphism in a pilot case control study of obesity as a state of metaflammation Scientific Reports Inflammation Leptin Metabolic syndrome Obesity TLR2 TNF-α |
| title | Serum levels of Wnt5a in Egyptian women with obesity and their association with toll like receptor 2 Arg753Gln gene polymorphism in a pilot case control study of obesity as a state of metaflammation |
| title_full | Serum levels of Wnt5a in Egyptian women with obesity and their association with toll like receptor 2 Arg753Gln gene polymorphism in a pilot case control study of obesity as a state of metaflammation |
| title_fullStr | Serum levels of Wnt5a in Egyptian women with obesity and their association with toll like receptor 2 Arg753Gln gene polymorphism in a pilot case control study of obesity as a state of metaflammation |
| title_full_unstemmed | Serum levels of Wnt5a in Egyptian women with obesity and their association with toll like receptor 2 Arg753Gln gene polymorphism in a pilot case control study of obesity as a state of metaflammation |
| title_short | Serum levels of Wnt5a in Egyptian women with obesity and their association with toll like receptor 2 Arg753Gln gene polymorphism in a pilot case control study of obesity as a state of metaflammation |
| title_sort | serum levels of wnt5a in egyptian women with obesity and their association with toll like receptor 2 arg753gln gene polymorphism in a pilot case control study of obesity as a state of metaflammation |
| topic | Inflammation Leptin Metabolic syndrome Obesity TLR2 TNF-α |
| url | https://doi.org/10.1038/s41598-025-85470-9 |
| work_keys_str_mv | AT shaimaahfouad serumlevelsofwnt5ainegyptianwomenwithobesityandtheirassociationwithtolllikereceptor2arg753glngenepolymorphisminapilotcasecontrolstudyofobesityasastateofmetaflammation AT maieldeeb serumlevelsofwnt5ainegyptianwomenwithobesityandtheirassociationwithtolllikereceptor2arg753glngenepolymorphisminapilotcasecontrolstudyofobesityasastateofmetaflammation AT shereenabaioumy serumlevelsofwnt5ainegyptianwomenwithobesityandtheirassociationwithtolllikereceptor2arg753glngenepolymorphisminapilotcasecontrolstudyofobesityasastateofmetaflammation AT saraitaha serumlevelsofwnt5ainegyptianwomenwithobesityandtheirassociationwithtolllikereceptor2arg753glngenepolymorphisminapilotcasecontrolstudyofobesityasastateofmetaflammation AT rehabaliibrahim serumlevelsofwnt5ainegyptianwomenwithobesityandtheirassociationwithtolllikereceptor2arg753glngenepolymorphisminapilotcasecontrolstudyofobesityasastateofmetaflammation AT ayaelgendy serumlevelsofwnt5ainegyptianwomenwithobesityandtheirassociationwithtolllikereceptor2arg753glngenepolymorphisminapilotcasecontrolstudyofobesityasastateofmetaflammation AT azzaomran serumlevelsofwnt5ainegyptianwomenwithobesityandtheirassociationwithtolllikereceptor2arg753glngenepolymorphisminapilotcasecontrolstudyofobesityasastateofmetaflammation AT marwahamdy serumlevelsofwnt5ainegyptianwomenwithobesityandtheirassociationwithtolllikereceptor2arg753glngenepolymorphisminapilotcasecontrolstudyofobesityasastateofmetaflammation AT raedaalharbi serumlevelsofwnt5ainegyptianwomenwithobesityandtheirassociationwithtolllikereceptor2arg753glngenepolymorphisminapilotcasecontrolstudyofobesityasastateofmetaflammation AT abdulmajeedaasindi serumlevelsofwnt5ainegyptianwomenwithobesityandtheirassociationwithtolllikereceptor2arg753glngenepolymorphisminapilotcasecontrolstudyofobesityasastateofmetaflammation AT sylviawroman serumlevelsofwnt5ainegyptianwomenwithobesityandtheirassociationwithtolllikereceptor2arg753glngenepolymorphisminapilotcasecontrolstudyofobesityasastateofmetaflammation |