Electrochemical Assessment of Microbial Activity Using PEDOT:PSS-Immobilized Cells
This study presents a microbial sensing device that employs a poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) matrix to immobilize viable and metabolically <i>Escherichia coli</i> cells. This device enables the monitoring of microorganism metabolic activity in response...
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MDPI AG
2025-06-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/13/6/211 |
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| author | N. Vigués C. Cantallops-Vilà J. Mas |
| author_facet | N. Vigués C. Cantallops-Vilà J. Mas |
| author_sort | N. Vigués |
| collection | DOAJ |
| description | This study presents a microbial sensing device that employs a poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) matrix to immobilize viable and metabolically <i>Escherichia coli</i> cells. This device enables the monitoring of microorganism metabolic activity in response to external stimuli such as variations in carbon sources or exposure to inhibitory or toxic compounds. PEDOT:PSS, a conductive and chemically stable polymer, was electrodeposited onto screen-printed electrodes, successfully entrapping approximately 1.26 × 10<sup>7</sup> cells per electrode. The confocal microscopy of Live/Dead-stained samples confirmed a uniform cell distribution and an average viability of ~78%. Ferricyanide respirometry validated the metabolic activity of the immobilized cells. The biosensor’s performance was evaluated using 3,5-dichlorophenol (3,5-DCP) as a reference toxicant. The observed inhibition of microbial activity correlated with 3,5-DCP concentration, yielding a half-maximal effective concentration (EC<sub>50</sub>) of 9 ppm, consistent with the literature values. |
| format | Article |
| id | doaj-art-1545d7efd45f49dd9a01c32da247b326 |
| institution | OA Journals |
| issn | 2227-9040 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj-art-1545d7efd45f49dd9a01c32da247b3262025-08-20T02:24:34ZengMDPI AGChemosensors2227-90402025-06-0113621110.3390/chemosensors13060211Electrochemical Assessment of Microbial Activity Using PEDOT:PSS-Immobilized CellsN. Vigués0C. Cantallops-Vilà1J. Mas2Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Genetics and Microbiology, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Genetics and Microbiology, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainThis study presents a microbial sensing device that employs a poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) matrix to immobilize viable and metabolically <i>Escherichia coli</i> cells. This device enables the monitoring of microorganism metabolic activity in response to external stimuli such as variations in carbon sources or exposure to inhibitory or toxic compounds. PEDOT:PSS, a conductive and chemically stable polymer, was electrodeposited onto screen-printed electrodes, successfully entrapping approximately 1.26 × 10<sup>7</sup> cells per electrode. The confocal microscopy of Live/Dead-stained samples confirmed a uniform cell distribution and an average viability of ~78%. Ferricyanide respirometry validated the metabolic activity of the immobilized cells. The biosensor’s performance was evaluated using 3,5-dichlorophenol (3,5-DCP) as a reference toxicant. The observed inhibition of microbial activity correlated with 3,5-DCP concentration, yielding a half-maximal effective concentration (EC<sub>50</sub>) of 9 ppm, consistent with the literature values.https://www.mdpi.com/2227-9040/13/6/211PEDOT:PSScell immobilization<i>Escherichia coli</i>metabolic activitymicrobial activityferricyanide respirometry |
| spellingShingle | N. Vigués C. Cantallops-Vilà J. Mas Electrochemical Assessment of Microbial Activity Using PEDOT:PSS-Immobilized Cells Chemosensors PEDOT:PSS cell immobilization <i>Escherichia coli</i> metabolic activity microbial activity ferricyanide respirometry |
| title | Electrochemical Assessment of Microbial Activity Using PEDOT:PSS-Immobilized Cells |
| title_full | Electrochemical Assessment of Microbial Activity Using PEDOT:PSS-Immobilized Cells |
| title_fullStr | Electrochemical Assessment of Microbial Activity Using PEDOT:PSS-Immobilized Cells |
| title_full_unstemmed | Electrochemical Assessment of Microbial Activity Using PEDOT:PSS-Immobilized Cells |
| title_short | Electrochemical Assessment of Microbial Activity Using PEDOT:PSS-Immobilized Cells |
| title_sort | electrochemical assessment of microbial activity using pedot pss immobilized cells |
| topic | PEDOT:PSS cell immobilization <i>Escherichia coli</i> metabolic activity microbial activity ferricyanide respirometry |
| url | https://www.mdpi.com/2227-9040/13/6/211 |
| work_keys_str_mv | AT nvigues electrochemicalassessmentofmicrobialactivityusingpedotpssimmobilizedcells AT ccantallopsvila electrochemicalassessmentofmicrobialactivityusingpedotpssimmobilizedcells AT jmas electrochemicalassessmentofmicrobialactivityusingpedotpssimmobilizedcells |