The genetic context of blaIMP varies among bacterial families from One Health sources.
The blaIMP resistance gene encodes a metallo-beta-lactamase in bacteria, which confers reduced susceptibility or resistance to all the beta-lactams, including carbapenems which are critical for treating life-threatening infections. The dissemination of blaIMP among various taxonomic families shows t...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0327200 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850063217722654720 |
|---|---|
| author | Susan Vaughn Grooters Dixie F Mollenkopf Thomas E Wittum |
| author_facet | Susan Vaughn Grooters Dixie F Mollenkopf Thomas E Wittum |
| author_sort | Susan Vaughn Grooters |
| collection | DOAJ |
| description | The blaIMP resistance gene encodes a metallo-beta-lactamase in bacteria, which confers reduced susceptibility or resistance to all the beta-lactams, including carbapenems which are critical for treating life-threatening infections. The dissemination of blaIMP among various taxonomic families shows the diversity and range of horizontal gene transfer. Using short-read whole genome sequencing and bioinformatic tools, we determined the genetic motifs surrounding blaIMP present in 32 bacterial isolates recovered from environmental sources and agriculture facilities. blaIMP can be located extra-chromosomally on plasmids or within incomplete and complete Tn7 chromosomal structures. We identified a complete Tn7 transposon harboring the blaIMP-27 gene cassette within a class 2 integron located in chromosomal contigs of Shewanella spp. and Providencia spp. Acinetobacter spp. isolates were observed with truncated and incomplete Tn7 transposons, while conserving the class 2 integron and resistance gene cassettes. Additionally, IncQ1 plasmids carried by Proteus spp., Escherichia coli, and other Enterobacteriaceae spp. harbored class 2 integrons with blaIMP-64 and sat2 resistance gene cassettes. In an Acidovorax sp. isolate, blaIMP-27 and sat2 gene cassettes were found associated with an insertion sequence, ISL3 transposase, in an RP4 plasmid. The conserved structure of Tn7 in Shewanella spp. and Providencia spp. is consistent with these species being potential reservoirs from which other bacterial species have acquired partial Tn7 motifs, and the blaIMP-27 gene cassette. These data contribute to a broader understanding of the dissemination and temporality of blaIMP alleles and their mobile genetic elements. |
| format | Article |
| id | doaj-art-1543d18b310a43f2b9fb51add8a741b9 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-1543d18b310a43f2b9fb51add8a741b92025-08-20T02:49:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01207e032720010.1371/journal.pone.0327200The genetic context of blaIMP varies among bacterial families from One Health sources.Susan Vaughn GrootersDixie F MollenkopfThomas E WittumThe blaIMP resistance gene encodes a metallo-beta-lactamase in bacteria, which confers reduced susceptibility or resistance to all the beta-lactams, including carbapenems which are critical for treating life-threatening infections. The dissemination of blaIMP among various taxonomic families shows the diversity and range of horizontal gene transfer. Using short-read whole genome sequencing and bioinformatic tools, we determined the genetic motifs surrounding blaIMP present in 32 bacterial isolates recovered from environmental sources and agriculture facilities. blaIMP can be located extra-chromosomally on plasmids or within incomplete and complete Tn7 chromosomal structures. We identified a complete Tn7 transposon harboring the blaIMP-27 gene cassette within a class 2 integron located in chromosomal contigs of Shewanella spp. and Providencia spp. Acinetobacter spp. isolates were observed with truncated and incomplete Tn7 transposons, while conserving the class 2 integron and resistance gene cassettes. Additionally, IncQ1 plasmids carried by Proteus spp., Escherichia coli, and other Enterobacteriaceae spp. harbored class 2 integrons with blaIMP-64 and sat2 resistance gene cassettes. In an Acidovorax sp. isolate, blaIMP-27 and sat2 gene cassettes were found associated with an insertion sequence, ISL3 transposase, in an RP4 plasmid. The conserved structure of Tn7 in Shewanella spp. and Providencia spp. is consistent with these species being potential reservoirs from which other bacterial species have acquired partial Tn7 motifs, and the blaIMP-27 gene cassette. These data contribute to a broader understanding of the dissemination and temporality of blaIMP alleles and their mobile genetic elements.https://doi.org/10.1371/journal.pone.0327200 |
| spellingShingle | Susan Vaughn Grooters Dixie F Mollenkopf Thomas E Wittum The genetic context of blaIMP varies among bacterial families from One Health sources. PLoS ONE |
| title | The genetic context of blaIMP varies among bacterial families from One Health sources. |
| title_full | The genetic context of blaIMP varies among bacterial families from One Health sources. |
| title_fullStr | The genetic context of blaIMP varies among bacterial families from One Health sources. |
| title_full_unstemmed | The genetic context of blaIMP varies among bacterial families from One Health sources. |
| title_short | The genetic context of blaIMP varies among bacterial families from One Health sources. |
| title_sort | genetic context of blaimp varies among bacterial families from one health sources |
| url | https://doi.org/10.1371/journal.pone.0327200 |
| work_keys_str_mv | AT susanvaughngrooters thegeneticcontextofblaimpvariesamongbacterialfamiliesfromonehealthsources AT dixiefmollenkopf thegeneticcontextofblaimpvariesamongbacterialfamiliesfromonehealthsources AT thomasewittum thegeneticcontextofblaimpvariesamongbacterialfamiliesfromonehealthsources AT susanvaughngrooters geneticcontextofblaimpvariesamongbacterialfamiliesfromonehealthsources AT dixiefmollenkopf geneticcontextofblaimpvariesamongbacterialfamiliesfromonehealthsources AT thomasewittum geneticcontextofblaimpvariesamongbacterialfamiliesfromonehealthsources |