Assessment of blood sampling time points to determine the relative bioavailability of ruminally protected methionine supplements using the plasma free amino acid dose-response technique
The calculation of the relative bioavailability (RBV) of rumen-protected AA supplements using the plasma free AA dose-response technique currently relies on blood samples obtained 2, 4, 6, and 8 h after the 0500 h feeding during the last 3 d of each period in Latin square experiments with cows fed e...
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Elsevier
2024-11-01
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| Series: | JDS Communications |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666910224000486 |
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| author | Nancy L. Whitehouse Devan L. Chirgwin Charles G. Schwab Daniel Luchini Nelson Lobos André F. Brito |
| author_facet | Nancy L. Whitehouse Devan L. Chirgwin Charles G. Schwab Daniel Luchini Nelson Lobos André F. Brito |
| author_sort | Nancy L. Whitehouse |
| collection | DOAJ |
| description | The calculation of the relative bioavailability (RBV) of rumen-protected AA supplements using the plasma free AA dose-response technique currently relies on blood samples obtained 2, 4, 6, and 8 h after the 0500 h feeding during the last 3 d of each period in Latin square experiments with cows fed every 8 h (0500, 1300, and 2100 h). The objective of this study was to determine if this current blood sampling protocol captures the changes that may occur in plasma Met concentrations within a 24-h day to adequately determine the RBV of Met from Smartamine M (SM). Five multiparous lactating Holstein cows were used in a 5 × 5 Latin square design with 7-d periods. Treatments were (1) control (abomasal infusion of tap water), (2) 12 g/d of abomasally infused dl-Met, (3) 24 g/d of abomasally infused dl-Met, (4) 15 g/d of fed Met (20 g/d of SM), and (5) 30 g/d of fed Met (40 g/d of SM). Blood samples were collected via jugular catheters every 2 h after the 0500 h feeding starting on d 5 and ending on d 7 of each period. Plasma Met analysis was conducted using gas chromatography after chloroformate derivatization. Plasma Met concentration was averaged across days for 2–8 h after the 0500 h feeding, 2–8 h after the 1300 h feeding, 2–8 h after the 2100 h feeding, and 2–24 h after the 0500 h feeding. In addition, plasma Met concentration was regressed on 0, 12, and 24 g of infused dl-Met and 0, 15, and 30 g of fed Met. The calculated RBV of Met from SM averaged 83.8%, 83.6%, 87.4%, and 83.0% for the 2–8 h, 10–16 h, 18–24 h, and 2–24 h sampling periods, respectively. The similarity in the estimations of RBV for the 2–8 h and 2–24 h sampling periods indicates that our original blood sampling protocol seems reliable for determining the RBV of ruminally protected Met products. |
| format | Article |
| id | doaj-art-15385be40b6641bea77b72edb7b80aae |
| institution | OA Journals |
| issn | 2666-9102 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JDS Communications |
| spelling | doaj-art-15385be40b6641bea77b72edb7b80aae2025-08-20T02:32:41ZengElsevierJDS Communications2666-91022024-11-015653954210.3168/jdsc.2023-0508Assessment of blood sampling time points to determine the relative bioavailability of ruminally protected methionine supplements using the plasma free amino acid dose-response techniqueNancy L. Whitehouse0Devan L. Chirgwin1Charles G. Schwab2Daniel Luchini3Nelson Lobos4André F. Brito5Department of Agriculture, Nutrition, and Food Systems, University of New Hampshire, Durham, NH 03824Department of Agriculture, Nutrition, and Food Systems, University of New Hampshire, Durham, NH 03824Department of Agriculture, Nutrition, and Food Systems, University of New Hampshire, Durham, NH 03824; Schwab Consulting LLC, Boscobel, WI 53805Adisseo USA Inc., Alpharetta, GA 30022Pioneer Hi-Bred International Inc., Johnston, IA 50131Department of Agriculture, Nutrition, and Food Systems, University of New Hampshire, Durham, NH 03824; Corresponding authorThe calculation of the relative bioavailability (RBV) of rumen-protected AA supplements using the plasma free AA dose-response technique currently relies on blood samples obtained 2, 4, 6, and 8 h after the 0500 h feeding during the last 3 d of each period in Latin square experiments with cows fed every 8 h (0500, 1300, and 2100 h). The objective of this study was to determine if this current blood sampling protocol captures the changes that may occur in plasma Met concentrations within a 24-h day to adequately determine the RBV of Met from Smartamine M (SM). Five multiparous lactating Holstein cows were used in a 5 × 5 Latin square design with 7-d periods. Treatments were (1) control (abomasal infusion of tap water), (2) 12 g/d of abomasally infused dl-Met, (3) 24 g/d of abomasally infused dl-Met, (4) 15 g/d of fed Met (20 g/d of SM), and (5) 30 g/d of fed Met (40 g/d of SM). Blood samples were collected via jugular catheters every 2 h after the 0500 h feeding starting on d 5 and ending on d 7 of each period. Plasma Met analysis was conducted using gas chromatography after chloroformate derivatization. Plasma Met concentration was averaged across days for 2–8 h after the 0500 h feeding, 2–8 h after the 1300 h feeding, 2–8 h after the 2100 h feeding, and 2–24 h after the 0500 h feeding. In addition, plasma Met concentration was regressed on 0, 12, and 24 g of infused dl-Met and 0, 15, and 30 g of fed Met. The calculated RBV of Met from SM averaged 83.8%, 83.6%, 87.4%, and 83.0% for the 2–8 h, 10–16 h, 18–24 h, and 2–24 h sampling periods, respectively. The similarity in the estimations of RBV for the 2–8 h and 2–24 h sampling periods indicates that our original blood sampling protocol seems reliable for determining the RBV of ruminally protected Met products.http://www.sciencedirect.com/science/article/pii/S2666910224000486 |
| spellingShingle | Nancy L. Whitehouse Devan L. Chirgwin Charles G. Schwab Daniel Luchini Nelson Lobos André F. Brito Assessment of blood sampling time points to determine the relative bioavailability of ruminally protected methionine supplements using the plasma free amino acid dose-response technique JDS Communications |
| title | Assessment of blood sampling time points to determine the relative bioavailability of ruminally protected methionine supplements using the plasma free amino acid dose-response technique |
| title_full | Assessment of blood sampling time points to determine the relative bioavailability of ruminally protected methionine supplements using the plasma free amino acid dose-response technique |
| title_fullStr | Assessment of blood sampling time points to determine the relative bioavailability of ruminally protected methionine supplements using the plasma free amino acid dose-response technique |
| title_full_unstemmed | Assessment of blood sampling time points to determine the relative bioavailability of ruminally protected methionine supplements using the plasma free amino acid dose-response technique |
| title_short | Assessment of blood sampling time points to determine the relative bioavailability of ruminally protected methionine supplements using the plasma free amino acid dose-response technique |
| title_sort | assessment of blood sampling time points to determine the relative bioavailability of ruminally protected methionine supplements using the plasma free amino acid dose response technique |
| url | http://www.sciencedirect.com/science/article/pii/S2666910224000486 |
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