PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants

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Main Authors: Jinlong Ai, Jinhai Deng, Jingjing Hu, Xingxiang Pu, Tongyan Yuan, Yuling Teng, Han Li, Bojie Chen, Jinlian Du, Ling Jiang, Xiaoyan Chen, Erhu Xiong, Ronghua Yang
Format: Article
Language:English
Published: American Chemical Society 2025-02-01
Series:JACS Au
Online Access:https://doi.org/10.1021/jacsau.5c00011
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author Jinlong Ai
Jinhai Deng
Jingjing Hu
Xingxiang Pu
Tongyan Yuan
Yuling Teng
Han Li
Bojie Chen
Jinlian Du
Ling Jiang
Xiaoyan Chen
Erhu Xiong
Ronghua Yang
author_facet Jinlong Ai
Jinhai Deng
Jingjing Hu
Xingxiang Pu
Tongyan Yuan
Yuling Teng
Han Li
Bojie Chen
Jinlian Du
Ling Jiang
Xiaoyan Chen
Erhu Xiong
Ronghua Yang
author_sort Jinlong Ai
collection DOAJ
format Article
id doaj-art-152d0219af4c4dc2b368814098f19ed2
institution DOAJ
issn 2691-3704
language English
publishDate 2025-02-01
publisher American Chemical Society
record_format Article
series JACS Au
spelling doaj-art-152d0219af4c4dc2b368814098f19ed22025-08-20T02:41:40ZengAmerican Chemical SocietyJACS Au2691-37042025-02-01531392140110.1021/jacsau.5c00011PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide VariantsJinlong Ai0Jinhai Deng1Jingjing Hu2Xingxiang Pu3Tongyan Yuan4Yuling Teng5Han Li6Bojie Chen7Jinlian Du8Ling Jiang9Xiaoyan Chen10Erhu Xiong11Ronghua Yang12Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education), Institute of Interdisciplinary Studies, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, ChinaRichard Dimbleby Laboratory of Cancer Research, School of Cancer & Pharmaceutical Sciences, King’s College London, London, U.K.Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education), Institute of Interdisciplinary Studies, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, ChinaDepartment of Medical Oncology, Lung Cancer and Gastrointestinal Unit, Hunan Cancer Hospital & The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, ChinaKey Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education), Institute of Interdisciplinary Studies, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, ChinaKey Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education), Institute of Interdisciplinary Studies, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, ChinaKey Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education), Institute of Interdisciplinary Studies, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, ChinaKey Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education), Institute of Interdisciplinary Studies, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, ChinaKey Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education), Institute of Interdisciplinary Studies, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, ChinaKey Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education), Institute of Interdisciplinary Studies, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, ChinaDepartment of Medical Oncology, Lung Cancer and Gastrointestinal Unit, Hunan Cancer Hospital & The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, ChinaKey Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education), Institute of Interdisciplinary Studies, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, ChinaKey Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education), Institute of Interdisciplinary Studies, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, Chinahttps://doi.org/10.1021/jacsau.5c00011
spellingShingle Jinlong Ai
Jinhai Deng
Jingjing Hu
Xingxiang Pu
Tongyan Yuan
Yuling Teng
Han Li
Bojie Chen
Jinlian Du
Ling Jiang
Xiaoyan Chen
Erhu Xiong
Ronghua Yang
PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants
JACS Au
title PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants
title_full PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants
title_fullStr PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants
title_full_unstemmed PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants
title_short PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants
title_sort pam independent crispr cas12a system for specific assays of single nucleotide variants
url https://doi.org/10.1021/jacsau.5c00011
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