Profile of outer membrane proteins of carbapenem-resistant Gram-negative bacilli in Ghana
Abstract Objective Carbapenem resistance is a major global health threat, but insights on its molecular determinants are scanty in sub-Saharan Africa, the predominant global antimicrobial resistance hotspot. This study aimed to profile outer membrane proteins (OMPs) of 111 carbapenem-resistant Gram-...
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2025-02-01
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| Online Access: | https://doi.org/10.1186/s13104-024-07070-6 |
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| author | Francis S. Codjoe Fleischer C. N. Kotey Eric S. Donkor |
| author_facet | Francis S. Codjoe Fleischer C. N. Kotey Eric S. Donkor |
| author_sort | Francis S. Codjoe |
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| description | Abstract Objective Carbapenem resistance is a major global health threat, but insights on its molecular determinants are scanty in sub-Saharan Africa, the predominant global antimicrobial resistance hotspot. This study aimed to profile outer membrane proteins (OMPs) of 111 carbapenem-resistant Gram-negative bacteria recovered from a broad spectrum of clinical specimens from Ghana. Results The OMPs of Pseudomonas aeruginosa produced decreased amounts of OprD or the porin was completely lost, except in 5.9% (n = 3) of the isolates which had high-level porins. For Acinetobacter baumannii, 96.8% (n = 30) expressed loss of OprF. One carbapenemase non-PCR-positive isolate with high-level porin expression was observed. In A. baumannii, the major and significant band on SDS-PAGE was ~ 35 kDa. There were substantial numbers of unrelated porin expression among the isolates. Particularly, OmpC/F or OmpK35/37 expression was deficient. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis for Escherichia coli, Klebsiella aerogenes, Klebsiella pneumoniae, and other Enterobacterales had OmpC and OmpF absent or markedly reduced compared to the control strains. Overall, porin loss was a major mechanism underlying carbapenem resistance among the isolates, suggesting that in carbapenem-resistant organisms that seem to lack known carbapenem resistance genes, porin loss may be the underlying carbapenem resistance mechanism. |
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| id | doaj-art-152c968eb7384c6bbfe541b04c0ebe8e |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
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| spelling | doaj-art-152c968eb7384c6bbfe541b04c0ebe8e2025-02-02T12:07:03ZengBMCBMC Research Notes1756-05002025-02-011811810.1186/s13104-024-07070-6Profile of outer membrane proteins of carbapenem-resistant Gram-negative bacilli in GhanaFrancis S. Codjoe0Fleischer C. N. Kotey1Eric S. Donkor2Department of Medical Laboratory Sciences, School of Biomedical & Allied Health Sciences, College of Health Sciences, University of GhanaDepartment of Medical Microbiology, University of Ghana Medical SchoolDepartment of Medical Microbiology, University of Ghana Medical SchoolAbstract Objective Carbapenem resistance is a major global health threat, but insights on its molecular determinants are scanty in sub-Saharan Africa, the predominant global antimicrobial resistance hotspot. This study aimed to profile outer membrane proteins (OMPs) of 111 carbapenem-resistant Gram-negative bacteria recovered from a broad spectrum of clinical specimens from Ghana. Results The OMPs of Pseudomonas aeruginosa produced decreased amounts of OprD or the porin was completely lost, except in 5.9% (n = 3) of the isolates which had high-level porins. For Acinetobacter baumannii, 96.8% (n = 30) expressed loss of OprF. One carbapenemase non-PCR-positive isolate with high-level porin expression was observed. In A. baumannii, the major and significant band on SDS-PAGE was ~ 35 kDa. There were substantial numbers of unrelated porin expression among the isolates. Particularly, OmpC/F or OmpK35/37 expression was deficient. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis for Escherichia coli, Klebsiella aerogenes, Klebsiella pneumoniae, and other Enterobacterales had OmpC and OmpF absent or markedly reduced compared to the control strains. Overall, porin loss was a major mechanism underlying carbapenem resistance among the isolates, suggesting that in carbapenem-resistant organisms that seem to lack known carbapenem resistance genes, porin loss may be the underlying carbapenem resistance mechanism.https://doi.org/10.1186/s13104-024-07070-6Outer membrane proteinsOMPsPorinsCarbapenem resistanceGram-negative bacilli |
| spellingShingle | Francis S. Codjoe Fleischer C. N. Kotey Eric S. Donkor Profile of outer membrane proteins of carbapenem-resistant Gram-negative bacilli in Ghana BMC Research Notes Outer membrane proteins OMPs Porins Carbapenem resistance Gram-negative bacilli |
| title | Profile of outer membrane proteins of carbapenem-resistant Gram-negative bacilli in Ghana |
| title_full | Profile of outer membrane proteins of carbapenem-resistant Gram-negative bacilli in Ghana |
| title_fullStr | Profile of outer membrane proteins of carbapenem-resistant Gram-negative bacilli in Ghana |
| title_full_unstemmed | Profile of outer membrane proteins of carbapenem-resistant Gram-negative bacilli in Ghana |
| title_short | Profile of outer membrane proteins of carbapenem-resistant Gram-negative bacilli in Ghana |
| title_sort | profile of outer membrane proteins of carbapenem resistant gram negative bacilli in ghana |
| topic | Outer membrane proteins OMPs Porins Carbapenem resistance Gram-negative bacilli |
| url | https://doi.org/10.1186/s13104-024-07070-6 |
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