Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15
Sixty bacterial strains isolated from the soils sample in the presence of organic solvent were screened for xylanase production. Among them, strain RSPP-15 showed the highest xylanase activity which was identified as Bacillus vallismortis. The isolate showed maximum xylanase production (3768 U/mL) i...
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| Format: | Article |
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Wiley
2015-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2015/986324 |
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| author | Rajeeva Gaur Soni Tiwari Priyanka Rai Versha Srivastava |
| author_facet | Rajeeva Gaur Soni Tiwari Priyanka Rai Versha Srivastava |
| author_sort | Rajeeva Gaur |
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| description | Sixty bacterial strains isolated from the soils sample in the presence of organic solvent were screened for xylanase production. Among them, strain RSPP-15 showed the highest xylanase activity which was identified as Bacillus vallismortis. The isolate showed maximum xylanase production (3768 U/mL) in the presence of birch wood xylan and beef extract at 55°C pH 7.0 within 48 h of incubation. The enzyme activity and stability were increased 181.5, 153.7, 147.2, 133.6, and 127.9% and 138.2, 119.3, 113.9, 109, and 104.5% in the presence of Co2+, Ca2+, Mg+2, Zn+2, and Fe+3 ions (10 mM). Xylanase activity and stability were strongly inhibited in the presence of Hg and Cu ions. The enzyme was also stable in the presence of 30% of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the xylanase stability, respectively. This isolate may be useful in several industrial applications owing to its thermotolerant and organic solvent resistance characteristics. |
| format | Article |
| id | doaj-art-1520d30fe6de42ee8c072dffb682982a |
| institution | OA Journals |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
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| series | International Journal of Polymer Science |
| spelling | doaj-art-1520d30fe6de42ee8c072dffb682982a2025-08-20T02:02:01ZengWileyInternational Journal of Polymer Science1687-94221687-94302015-01-01201510.1155/2015/986324986324Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15Rajeeva Gaur0Soni Tiwari1Priyanka Rai2Versha Srivastava3Department of Microbiology (Centre of Excellence), Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224 001, IndiaDepartment of Microbiology (Centre of Excellence), Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224 001, IndiaDepartment of Microbiology (Centre of Excellence), Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224 001, IndiaDepartment of Microbiology (Centre of Excellence), Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224 001, IndiaSixty bacterial strains isolated from the soils sample in the presence of organic solvent were screened for xylanase production. Among them, strain RSPP-15 showed the highest xylanase activity which was identified as Bacillus vallismortis. The isolate showed maximum xylanase production (3768 U/mL) in the presence of birch wood xylan and beef extract at 55°C pH 7.0 within 48 h of incubation. The enzyme activity and stability were increased 181.5, 153.7, 147.2, 133.6, and 127.9% and 138.2, 119.3, 113.9, 109, and 104.5% in the presence of Co2+, Ca2+, Mg+2, Zn+2, and Fe+3 ions (10 mM). Xylanase activity and stability were strongly inhibited in the presence of Hg and Cu ions. The enzyme was also stable in the presence of 30% of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the xylanase stability, respectively. This isolate may be useful in several industrial applications owing to its thermotolerant and organic solvent resistance characteristics.http://dx.doi.org/10.1155/2015/986324 |
| spellingShingle | Rajeeva Gaur Soni Tiwari Priyanka Rai Versha Srivastava Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15 International Journal of Polymer Science |
| title | Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15 |
| title_full | Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15 |
| title_fullStr | Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15 |
| title_full_unstemmed | Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15 |
| title_short | Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15 |
| title_sort | isolation production and characterization of thermotolerant xylanase from solvent tolerant bacillus vallismortis rspp 15 |
| url | http://dx.doi.org/10.1155/2015/986324 |
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