MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway
Osteosarcoma is the most common primary malignant bone tumor in children and adolescents with poor prognosis. MicroRNA-181a-5p (miR-181a-5p) is involved in the progression of various tumors; however, its role and underlying mechanism in osteosarcoma remains unclear. In this study, we found that miR-...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2022/3421600 |
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| _version_ | 1849308312190844928 |
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| author | Chen Sun Chi Chen Zhen Chen Jun Guo Zhi-Hong Yu Wei Qian Fen Ai Liang Xiao Xiao Guo |
| author_facet | Chen Sun Chi Chen Zhen Chen Jun Guo Zhi-Hong Yu Wei Qian Fen Ai Liang Xiao Xiao Guo |
| author_sort | Chen Sun |
| collection | DOAJ |
| description | Osteosarcoma is the most common primary malignant bone tumor in children and adolescents with poor prognosis. MicroRNA-181a-5p (miR-181a-5p) is involved in the progression of various tumors; however, its role and underlying mechanism in osteosarcoma remains unclear. In this study, we found that miR-181a-5p was upregulated in human osteosarcoma cells and tissues. miR-181a-5p mimic significantly promoted, while miR-181a-5p inhibitor blocked the proliferation, colony formation, migration, invasion, and cell cycle progression of osteosarcoma cells. Mechanistically, miR-181a-5p bound to the 3′-untranslational region of phosphatase and tensin homolog (PTEN) and reduced its protein expression, thereby activating protein kinase B (PKB/AKT) pathway. Either PTEN overexpression or AKT inhibition notably blocked the tumor-promoting effects of miR-181a-5p. Moreover, we observed that miR-181a-5p mimic further inhibited growth of human osteosarcoma cells in the presence of adriamycin or cisplatin. Overall, miR-181a-5p promotes osteosarcoma progression via PTEN/AKT pathway and it is a promising therapeutic target to treat osteosarcoma. |
| format | Article |
| id | doaj-art-1515d22035e4485e8785c836b41d6fc5 |
| institution | Kabale University |
| issn | 2210-7185 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-1515d22035e4485e8785c836b41d6fc52025-08-20T03:54:29ZengWileyAnalytical Cellular Pathology2210-71852022-01-01202210.1155/2022/3421600MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT PathwayChen Sun0Chi Chen1Zhen Chen2Jun Guo3Zhi-Hong Yu4Wei Qian5Fen Ai6Liang Xiao7Xiao Guo8Department of Orthopedic SurgeryDepartment of Orthopedic SurgeryDepartment of EmergencyDepartment of Orthopedic SurgeryDepartment of Orthopedic SurgeryDepartment of Orthopedic SurgeryDepartment of EmergencyDepartment of Orthopedic SurgeryDepartment of Orthopedic SurgeryOsteosarcoma is the most common primary malignant bone tumor in children and adolescents with poor prognosis. MicroRNA-181a-5p (miR-181a-5p) is involved in the progression of various tumors; however, its role and underlying mechanism in osteosarcoma remains unclear. In this study, we found that miR-181a-5p was upregulated in human osteosarcoma cells and tissues. miR-181a-5p mimic significantly promoted, while miR-181a-5p inhibitor blocked the proliferation, colony formation, migration, invasion, and cell cycle progression of osteosarcoma cells. Mechanistically, miR-181a-5p bound to the 3′-untranslational region of phosphatase and tensin homolog (PTEN) and reduced its protein expression, thereby activating protein kinase B (PKB/AKT) pathway. Either PTEN overexpression or AKT inhibition notably blocked the tumor-promoting effects of miR-181a-5p. Moreover, we observed that miR-181a-5p mimic further inhibited growth of human osteosarcoma cells in the presence of adriamycin or cisplatin. Overall, miR-181a-5p promotes osteosarcoma progression via PTEN/AKT pathway and it is a promising therapeutic target to treat osteosarcoma.http://dx.doi.org/10.1155/2022/3421600 |
| spellingShingle | Chen Sun Chi Chen Zhen Chen Jun Guo Zhi-Hong Yu Wei Qian Fen Ai Liang Xiao Xiao Guo MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway Analytical Cellular Pathology |
| title | MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway |
| title_full | MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway |
| title_fullStr | MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway |
| title_full_unstemmed | MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway |
| title_short | MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway |
| title_sort | microrna 181a 5p promotes osteosarcoma progression via pten akt pathway |
| url | http://dx.doi.org/10.1155/2022/3421600 |
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