Novel ecofriendly spectrophotometric methods for the determination of six dihydropyridines calcium channel blockers through derivatization with sulfophtalein dye: application to tablet analysis
Abstract Novel, «green» and simple visible spectrophotometric procedures for the determination of six dihydropyridines CCBs (amlodipine besylate (AML), lacidipine (LAC), levamlodipine besylate (LAML), nifedipine (NIF), nimodipine (NIM) and nitrendipine (NIT)) through derivatization with the sulfopht...
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BMC
2025-01-01
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| Series: | BMC Chemistry |
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| Online Access: | https://doi.org/10.1186/s13065-024-01378-x |
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| author | Mariana Horyn Liubomyr Kryskiw Tetyana Kucher Nadiya Zarivna Olha Poliak Liliya Logoyda |
| author_facet | Mariana Horyn Liubomyr Kryskiw Tetyana Kucher Nadiya Zarivna Olha Poliak Liliya Logoyda |
| author_sort | Mariana Horyn |
| collection | DOAJ |
| description | Abstract Novel, «green» and simple visible spectrophotometric procedures for the determination of six dihydropyridines CCBs (amlodipine besylate (AML), lacidipine (LAC), levamlodipine besylate (LAML), nifedipine (NIF), nimodipine (NIM) and nitrendipine (NIT)) through derivatization with the sulfophthalein dye bromophenol blue (BPB) have been developed. The optimal parameters for CCBs spectrophotometric analysis via complex formation using BPB were as follows: detection wavelength—596 nm, reaction time—5 min, ratio of reacting components—1:1, operating temperature—25 ± 2 °C. The concentration was linearly proportional to absorbance values in the range of 3.40—17.00 μg/mL (AML), 1.14—9.11 μg/mL (LAC), 1.14—9.08 μg/mL (LAML), 4.16—12.40 μg/mL (NIF), 0.84—5.86 μg/mL (NIM), 6.52—19.60 μg/mL (NIT). The developed methods are colorimetric and therefore does not require a UV instrument to quantify these drugs. The proposed approach was more efficient in terms of time reliability, sensitivity and «greenness» than other recorded spectrophotometric methods and can be easily implemented for routine pharmaceutical analysis. |
| format | Article |
| id | doaj-art-1502816301a54e028f8fab68e8e9a37d |
| institution | Kabale University |
| issn | 2661-801X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Chemistry |
| spelling | doaj-art-1502816301a54e028f8fab68e8e9a37d2025-01-12T12:06:40ZengBMCBMC Chemistry2661-801X2025-01-0119112010.1186/s13065-024-01378-xNovel ecofriendly spectrophotometric methods for the determination of six dihydropyridines calcium channel blockers through derivatization with sulfophtalein dye: application to tablet analysisMariana Horyn0Liubomyr Kryskiw1Tetyana Kucher2Nadiya Zarivna3Olha Poliak4Liliya Logoyda5Department of Pharmaceutical Chemistry, I. Horbachevsky Ternopil National Medical UniversityDepartment of Pharmaceutical Chemistry, I. Horbachevsky Ternopil National Medical UniversityDepartment of Pharmaceutical Chemistry, I. Horbachevsky Ternopil National Medical UniversityDepartment of Pharmaceutical Chemistry, I. Horbachevsky Ternopil National Medical UniversityDepartment of Pharmaceutical Chemistry, I. Horbachevsky Ternopil National Medical UniversityDepartment of Pharmaceutical Chemistry, I. Horbachevsky Ternopil National Medical UniversityAbstract Novel, «green» and simple visible spectrophotometric procedures for the determination of six dihydropyridines CCBs (amlodipine besylate (AML), lacidipine (LAC), levamlodipine besylate (LAML), nifedipine (NIF), nimodipine (NIM) and nitrendipine (NIT)) through derivatization with the sulfophthalein dye bromophenol blue (BPB) have been developed. The optimal parameters for CCBs spectrophotometric analysis via complex formation using BPB were as follows: detection wavelength—596 nm, reaction time—5 min, ratio of reacting components—1:1, operating temperature—25 ± 2 °C. The concentration was linearly proportional to absorbance values in the range of 3.40—17.00 μg/mL (AML), 1.14—9.11 μg/mL (LAC), 1.14—9.08 μg/mL (LAML), 4.16—12.40 μg/mL (NIF), 0.84—5.86 μg/mL (NIM), 6.52—19.60 μg/mL (NIT). The developed methods are colorimetric and therefore does not require a UV instrument to quantify these drugs. The proposed approach was more efficient in terms of time reliability, sensitivity and «greenness» than other recorded spectrophotometric methods and can be easily implemented for routine pharmaceutical analysis.https://doi.org/10.1186/s13065-024-01378-xCalcium channel blockersBromophenol blueSpectrophotometryAssayValidation |
| spellingShingle | Mariana Horyn Liubomyr Kryskiw Tetyana Kucher Nadiya Zarivna Olha Poliak Liliya Logoyda Novel ecofriendly spectrophotometric methods for the determination of six dihydropyridines calcium channel blockers through derivatization with sulfophtalein dye: application to tablet analysis BMC Chemistry Calcium channel blockers Bromophenol blue Spectrophotometry Assay Validation |
| title | Novel ecofriendly spectrophotometric methods for the determination of six dihydropyridines calcium channel blockers through derivatization with sulfophtalein dye: application to tablet analysis |
| title_full | Novel ecofriendly spectrophotometric methods for the determination of six dihydropyridines calcium channel blockers through derivatization with sulfophtalein dye: application to tablet analysis |
| title_fullStr | Novel ecofriendly spectrophotometric methods for the determination of six dihydropyridines calcium channel blockers through derivatization with sulfophtalein dye: application to tablet analysis |
| title_full_unstemmed | Novel ecofriendly spectrophotometric methods for the determination of six dihydropyridines calcium channel blockers through derivatization with sulfophtalein dye: application to tablet analysis |
| title_short | Novel ecofriendly spectrophotometric methods for the determination of six dihydropyridines calcium channel blockers through derivatization with sulfophtalein dye: application to tablet analysis |
| title_sort | novel ecofriendly spectrophotometric methods for the determination of six dihydropyridines calcium channel blockers through derivatization with sulfophtalein dye application to tablet analysis |
| topic | Calcium channel blockers Bromophenol blue Spectrophotometry Assay Validation |
| url | https://doi.org/10.1186/s13065-024-01378-x |
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