A casein kinase TaCK2α interacts with TaCK2β and contributes to wheat resistance to Puccinia triticina
Abstract Casein kinase 2 (CK2), a key multifunctional protein kinase in plant cells, is ubiquitously expressed and plays a crucial role in survival under various stress conditions. However, the role of CK2 in the interaction between wheat and Puccinia triticina (Pt) remains ambiguous. In our previou...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-08-01
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| Series: | Phytopathology Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42483-025-00352-2 |
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| Summary: | Abstract Casein kinase 2 (CK2), a key multifunctional protein kinase in plant cells, is ubiquitously expressed and plays a crucial role in survival under various stress conditions. However, the role of CK2 in the interaction between wheat and Puccinia triticina (Pt) remains ambiguous. In our previous study, a CK2 gene, known as TaCK2α, was identified in the near-isogenic wheat line TcLr19 inoculated with Pt through RNA-Sequence analysis. In the current study, quantitative real-time polymerase chain reaction (qPCR) analysis revealed that the expression of TaCK2α was upregulated by the Pt race THTS and signaling molecules, indicating its potential involvement in the wheat-Pt interaction, particularly in relation to salicylic acid (SA) signaling. Antifungal activity assays and virus-induced gene silencing (VIGS) analysis further validated the role of TaCK2α in regulating wheat resistance to Pt. Using a combination of pulldown assays and mass spectrometry (MS), 24 potential interacting targets of TaCK2α were identified in wheat. Among these targets, the interaction between TaCK2α and TaCK2β was confirmed through Yeast two-hybrid (Y2H) and Co-Immunoprecipitation (Co-IP). Additionally, the subcellular localization of TaCK2α was found to be altered by CK2β. TaCK2α plays a positive role in reactive oxygen species (ROS) induction and callose deposition in wheat, whereas TaCK2β contributes to the pattern-triggered immunity (PTI) response by interacting with TaCK2α. Collectively, our results demonstrate that TaCK2α targets TaCK2β to mediate wheat resistance against Pt through PTI, providing a solid basis for further investigation into the molecular mechanisms underlying CK2-dependent wheat resistance to biotic stress. |
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| ISSN: | 2524-4167 |