Rodent ischemic stroke models and their relevance in preclinical research

Abstract Stroke is a leading cause of death and disability worldwide, with ischemic stroke caused by an occluded vessel accounting for the majority of cases. Current treatments are limited to recanalization, either through thrombectomy or thrombolysis. Approved pharmacological interventions to suppr...

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Main Authors: Maria Thaysen, Emil Westi, Andrew N. Clarkson, Petrine Wellendorph, Mie Kristensen
Format: Article
Language:English
Published: Wiley 2024-12-01
Series:Neuroprotection
Subjects:
Online Access:https://doi.org/10.1002/nep3.62
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author Maria Thaysen
Emil Westi
Andrew N. Clarkson
Petrine Wellendorph
Mie Kristensen
author_facet Maria Thaysen
Emil Westi
Andrew N. Clarkson
Petrine Wellendorph
Mie Kristensen
author_sort Maria Thaysen
collection DOAJ
description Abstract Stroke is a leading cause of death and disability worldwide, with ischemic stroke caused by an occluded vessel accounting for the majority of cases. Current treatments are limited to recanalization, either through thrombectomy or thrombolysis. Approved pharmacological interventions to suppress stroke‐associated excitotoxicity and neuroinflammatory events, leading to brain tissue death, are still lacking. Although numerous preclinical studies have been performed, they have yet to be translated into clinically relevant interventions. First‐line preclinical in vivo studies include the use of rodent ischemic stroke models, which vary in terms of how well they replicate human stroke pathophysiology and phenotype (including the formation of blood clot, blood–brain barrier disruption, neuroinflammation, and edema generation). Thus, rodent ischemic stroke models must be carefully chosen according to the specific pharmacological intervention to be tested. In this review, we aimed to provide an overview of the five most commonly used rodent ischemic stroke models and critically assess their advantages and limitations, with a primary focus on the acute phases of stroke.
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spelling doaj-art-14e4ab7ffee04d26b0a7e7df9a8d578b2025-08-20T03:13:11ZengWileyNeuroprotection2770-72962770-730X2024-12-012429630910.1002/nep3.62Rodent ischemic stroke models and their relevance in preclinical researchMaria Thaysen0Emil Westi1Andrew N. Clarkson2Petrine Wellendorph3Mie Kristensen4Department of Pharmacy, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen DenmarkDepartment of Drug Design and Pharmacology, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen DenmarkREGENBIO Consultants Limited Dunedin New ZealandDepartment of Drug Design and Pharmacology, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen DenmarkDepartment of Pharmacy, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen DenmarkAbstract Stroke is a leading cause of death and disability worldwide, with ischemic stroke caused by an occluded vessel accounting for the majority of cases. Current treatments are limited to recanalization, either through thrombectomy or thrombolysis. Approved pharmacological interventions to suppress stroke‐associated excitotoxicity and neuroinflammatory events, leading to brain tissue death, are still lacking. Although numerous preclinical studies have been performed, they have yet to be translated into clinically relevant interventions. First‐line preclinical in vivo studies include the use of rodent ischemic stroke models, which vary in terms of how well they replicate human stroke pathophysiology and phenotype (including the formation of blood clot, blood–brain barrier disruption, neuroinflammation, and edema generation). Thus, rodent ischemic stroke models must be carefully chosen according to the specific pharmacological intervention to be tested. In this review, we aimed to provide an overview of the five most commonly used rodent ischemic stroke models and critically assess their advantages and limitations, with a primary focus on the acute phases of stroke.https://doi.org/10.1002/nep3.62animal modelsendothelin‐1 induced strokeexcitotoxicityischemic strokemiddle cerebral artery occlusionphotothrombotic stroke
spellingShingle Maria Thaysen
Emil Westi
Andrew N. Clarkson
Petrine Wellendorph
Mie Kristensen
Rodent ischemic stroke models and their relevance in preclinical research
Neuroprotection
animal models
endothelin‐1 induced stroke
excitotoxicity
ischemic stroke
middle cerebral artery occlusion
photothrombotic stroke
title Rodent ischemic stroke models and their relevance in preclinical research
title_full Rodent ischemic stroke models and their relevance in preclinical research
title_fullStr Rodent ischemic stroke models and their relevance in preclinical research
title_full_unstemmed Rodent ischemic stroke models and their relevance in preclinical research
title_short Rodent ischemic stroke models and their relevance in preclinical research
title_sort rodent ischemic stroke models and their relevance in preclinical research
topic animal models
endothelin‐1 induced stroke
excitotoxicity
ischemic stroke
middle cerebral artery occlusion
photothrombotic stroke
url https://doi.org/10.1002/nep3.62
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AT petrinewellendorph rodentischemicstrokemodelsandtheirrelevanceinpreclinicalresearch
AT miekristensen rodentischemicstrokemodelsandtheirrelevanceinpreclinicalresearch