Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig
Summary: The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter im...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725001807 |
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| author | Yasmin Eckstein Barbara Kessler Arne Hinrichs Istvan Novak Anne von Thaden Timo Lorenzen Birgit Rathkolb Armin Scholz Andreas Blutke Sietse-Jan Koopmans Martin Hrabĕ de Angelis Berit Østergaard Christoffersen Eckhard Wolf Simone Renner |
| author_facet | Yasmin Eckstein Barbara Kessler Arne Hinrichs Istvan Novak Anne von Thaden Timo Lorenzen Birgit Rathkolb Armin Scholz Andreas Blutke Sietse-Jan Koopmans Martin Hrabĕ de Angelis Berit Østergaard Christoffersen Eckhard Wolf Simone Renner |
| author_sort | Yasmin Eckstein |
| collection | DOAJ |
| description | Summary: The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis.For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-14b6b028bd94473f845cd24d44fbdc9e |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-14b6b028bd94473f845cd24d44fbdc9e2025-08-20T02:17:27ZengElsevierSTAR Protocols2666-16672025-06-016210377410.1016/j.xpro.2025.103774Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pigYasmin Eckstein0Barbara Kessler1Arne Hinrichs2Istvan Novak3Anne von Thaden4Timo Lorenzen5Birgit Rathkolb6Armin Scholz7Andreas Blutke8Sietse-Jan Koopmans9Martin Hrabĕ de Angelis10Berit Østergaard Christoffersen11Eckhard Wolf12Simone Renner13Chair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; Center for Innovative Medical Models (CiMM), LMU Munich, 85764 Oberschleißheim, GermanyChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; Center for Innovative Medical Models (CiMM), LMU Munich, 85764 Oberschleißheim, GermanyChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; Center for Innovative Medical Models (CiMM), LMU Munich, 85764 Oberschleißheim, GermanyChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; Center for Innovative Medical Models (CiMM), LMU Munich, 85764 Oberschleißheim, GermanyGerman Center for Neurodegenerative Diseases (DZNE), LMU Munich, 81377 Munich, GermanyInstitute of Veterinary Pathology at the Center for Clinical Veterinary Medicine, LMU Munich, 80539 Munich, GermanyChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; German Mouse Clinic (GMC) and Genome Analysis Center (GAC), Institute of Experimental Genetics, Helmholtz Munich, 85764 Neuherberg, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, GermanyLivestock Center Oberschleissheim, LMU Munich, 85764 Oberschleißheim, GermanyInstitute of Veterinary Pathology at the Center for Clinical Veterinary Medicine, LMU Munich, 80539 Munich, GermanyWageningen Livestock Research, Wageningen University & Research, Wageningen, the NetherlandsGerman Mouse Clinic (GMC) and Genome Analysis Center (GAC), Institute of Experimental Genetics, Helmholtz Munich, 85764 Neuherberg, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany; Chair of Experimental Genetics, TUM School of Life Sciences (SoLS), Technische Universität München, 85354 Freising, GermanyNovo Nordisk A/S, Maaloev, DenmarkChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; Center for Innovative Medical Models (CiMM), LMU Munich, 85764 Oberschleißheim, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany; Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU), LMU Munich, 81377 Munich, GermanyChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, 81377 Munich, Germany; Center for Innovative Medical Models (CiMM), LMU Munich, 85764 Oberschleißheim, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany; Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU), LMU Munich, 81377 Munich, Germany; Corresponding authorSummary: The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis.For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725001807MetabolismModel OrganismsBiotechnology and bioengineering |
| spellingShingle | Yasmin Eckstein Barbara Kessler Arne Hinrichs Istvan Novak Anne von Thaden Timo Lorenzen Birgit Rathkolb Armin Scholz Andreas Blutke Sietse-Jan Koopmans Martin Hrabĕ de Angelis Berit Østergaard Christoffersen Eckhard Wolf Simone Renner Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig STAR Protocols Metabolism Model Organisms Biotechnology and bioengineering |
| title | Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig |
| title_full | Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig |
| title_fullStr | Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig |
| title_full_unstemmed | Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig |
| title_short | Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig |
| title_sort | protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig |
| topic | Metabolism Model Organisms Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725001807 |
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