Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig

Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig

Summary: The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter im...

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Main Authors: Yasmin Eckstein, Barbara Kessler, Arne Hinrichs, Istvan Novak, Anne von Thaden, Timo Lorenzen, Birgit Rathkolb, Armin Scholz, Andreas Blutke, Sietse-Jan Koopmans, Martin Hrabĕ de Angelis, Berit Østergaard Christoffersen, Eckhard Wolf, Simone Renner
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Metabolism
Model Organisms
Biotechnology and bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001807
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Summary:Summary: The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis.For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

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