Dietary supplement of veratric acid alleviates liver steatosis and reduces abdominal fat deposition in broilers

Nonalcoholic fatty liver disease (NAFLD) and obesity are nutritional metabolic diseases that are prevalent in the poultry industry, and have a negative impact on its functioning. Veratric acid (VA) is a phenolic acid compound extracted from the Chinese herbal medicine Trollius chinensis Bunge, known...

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Main Authors: Tingli Pan, Siqi Liu, Qichao Liao, Yu Li, Yang Xiao, Yu Sun, Lei Zhou, Yixing Li
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Poultry Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0032579124009854
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author Tingli Pan
Siqi Liu
Qichao Liao
Yu Li
Yang Xiao
Yu Sun
Lei Zhou
Yixing Li
author_facet Tingli Pan
Siqi Liu
Qichao Liao
Yu Li
Yang Xiao
Yu Sun
Lei Zhou
Yixing Li
author_sort Tingli Pan
collection DOAJ
description Nonalcoholic fatty liver disease (NAFLD) and obesity are nutritional metabolic diseases that are prevalent in the poultry industry, and have a negative impact on its functioning. Veratric acid (VA) is a phenolic acid compound extracted from the Chinese herbal medicine Trollius chinensis Bunge, known for its anti-inflammatory and antioxidant properties. In this study, we used chicken hepatocytes (Leghorn male hepatoma cells) and treated with a mixture of oleic acid and palmitic acid as well as Yellow-feathered broilers fed a high-fat diet to examine the impact of VA on liver-lipid metabolism and deposition of abdominal fat. The results showed that VA (1μM) reduced the triglyceride and total cholesterol levels in the chicken hepatocytes (p < 0.05). In the broiler NAFLD model, VA significantly reduced liver TG levels (p < 0.05) without affecting growth performance. Dietary supplementation with 0.05% or 0.1% VA supplementation also significantly reduced the mRNA expression levels of key genes involved in the synthesis of fatty acids such as sterol regulatory element-binding protein 1c, fatty acid synthase, and acetyl-CoA carboxylase in broiler livers. In addition, 0.1% VA reduced abdominal fat accumulation and improved blood biochemical indexes in broilers. Network pharmacology analysis suggested that VA may participate in regulating fat metabolism in broilers via the proliferator-activated receptor signaling pathway. Taken together, the study results support VA as a candidate feed additive to provide a novel strategy for preventing NAFLD and excessive fat deposition in chickens.
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spelling doaj-art-14b5c8c13e404e1ea8c97133c140a5fc2025-08-20T02:37:45ZengElsevierPoultry Science0032-57912024-12-011031210440610.1016/j.psj.2024.104406Dietary supplement of veratric acid alleviates liver steatosis and reduces abdominal fat deposition in broilersTingli Pan0Siqi Liu1Qichao Liao2Yu Li3Yang Xiao4Yu Sun5Lei Zhou6Yixing Li7Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, 530004, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, 530004, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, 530004, ChinaInstitute of Digestive Disease, Guangxi Academy of Medical Sciences, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, ChinaInstitute of Digestive Disease, Guangxi Academy of Medical Sciences, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, ChinaInstitute of Digestive Disease, Guangxi Academy of Medical Sciences, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, ChinaInstitute of Digestive Disease, Guangxi Academy of Medical Sciences, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, 530004, China; Corresponding author:Nonalcoholic fatty liver disease (NAFLD) and obesity are nutritional metabolic diseases that are prevalent in the poultry industry, and have a negative impact on its functioning. Veratric acid (VA) is a phenolic acid compound extracted from the Chinese herbal medicine Trollius chinensis Bunge, known for its anti-inflammatory and antioxidant properties. In this study, we used chicken hepatocytes (Leghorn male hepatoma cells) and treated with a mixture of oleic acid and palmitic acid as well as Yellow-feathered broilers fed a high-fat diet to examine the impact of VA on liver-lipid metabolism and deposition of abdominal fat. The results showed that VA (1μM) reduced the triglyceride and total cholesterol levels in the chicken hepatocytes (p < 0.05). In the broiler NAFLD model, VA significantly reduced liver TG levels (p < 0.05) without affecting growth performance. Dietary supplementation with 0.05% or 0.1% VA supplementation also significantly reduced the mRNA expression levels of key genes involved in the synthesis of fatty acids such as sterol regulatory element-binding protein 1c, fatty acid synthase, and acetyl-CoA carboxylase in broiler livers. In addition, 0.1% VA reduced abdominal fat accumulation and improved blood biochemical indexes in broilers. Network pharmacology analysis suggested that VA may participate in regulating fat metabolism in broilers via the proliferator-activated receptor signaling pathway. Taken together, the study results support VA as a candidate feed additive to provide a novel strategy for preventing NAFLD and excessive fat deposition in chickens.http://www.sciencedirect.com/science/article/pii/S0032579124009854BroilerFat depositionNAFLDVeratric acid
spellingShingle Tingli Pan
Siqi Liu
Qichao Liao
Yu Li
Yang Xiao
Yu Sun
Lei Zhou
Yixing Li
Dietary supplement of veratric acid alleviates liver steatosis and reduces abdominal fat deposition in broilers
Poultry Science
Broiler
Fat deposition
NAFLD
Veratric acid
title Dietary supplement of veratric acid alleviates liver steatosis and reduces abdominal fat deposition in broilers
title_full Dietary supplement of veratric acid alleviates liver steatosis and reduces abdominal fat deposition in broilers
title_fullStr Dietary supplement of veratric acid alleviates liver steatosis and reduces abdominal fat deposition in broilers
title_full_unstemmed Dietary supplement of veratric acid alleviates liver steatosis and reduces abdominal fat deposition in broilers
title_short Dietary supplement of veratric acid alleviates liver steatosis and reduces abdominal fat deposition in broilers
title_sort dietary supplement of veratric acid alleviates liver steatosis and reduces abdominal fat deposition in broilers
topic Broiler
Fat deposition
NAFLD
Veratric acid
url http://www.sciencedirect.com/science/article/pii/S0032579124009854
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