Accurately Tunable AuNC‐ZIF Content Architecture Based on Coordination‐Dissociation Mechanism Enables Highly Brightness Dual‐Site Fluorescent Biosensor
Abstract The quantum yield and fluorescence intensity of gold nanocluster (AuNC) nanocarriers are critical parameters for developing ultrasensitive biosensors. In this study, AuNCs‐zeolitic‐imidazolate‐framework (Au‐ZIF) nanocomposites are systematically constructed by impregnating AuNCs onto the ZI...
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Wiley
2025-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202408400 |
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| author | Junyang Chen Yuqian Wang Runpu Shen Wei Li Sainan Gao Zhikang Xiao Qiyan Lv Xiaojie Song Jianzhong Xu Gaoxiang Xu Huifang Cui Zhaohui Li |
| author_facet | Junyang Chen Yuqian Wang Runpu Shen Wei Li Sainan Gao Zhikang Xiao Qiyan Lv Xiaojie Song Jianzhong Xu Gaoxiang Xu Huifang Cui Zhaohui Li |
| author_sort | Junyang Chen |
| collection | DOAJ |
| description | Abstract The quantum yield and fluorescence intensity of gold nanocluster (AuNC) nanocarriers are critical parameters for developing ultrasensitive biosensors. In this study, AuNCs‐zeolitic‐imidazolate‐framework (Au‐ZIF) nanocomposites are systematically constructed by impregnating AuNCs onto the ZIF‐8 surface through a coordination‐dissociation mechanism, resulting in a dual‐site fluorescence‐loaded structure. In this configuration, AuNCs are anchored to the external surface while the integrity of the inner cavity remains intact. The surface of ZIF‐8 induces a confinement effect on the configuration and electrons of AuNCs, significantly enhancing luminescence (18‐fold increase). The quantum yield of AuNCs exhibits an increase of more than 13‐fold, from 2.80% to 38.1%. This approach demonstrates broad applicability and maintains strong fluorescence across different ZIFs. Additionally, a novel nanocomposite, Au‐ZIF@carbon‐dots (CDs), is synthesized by encapsulating CDs into the inner cavity of Au‐ZIF. A ratiometric fluorescence detection platform is subsequently developed and incorporated into hydrogels for the quantitative detection of the pesticide triazophos. By employing an image‐processing algorithm, quantitative detection is achieved with a detection limit of 0.07 ng mL⁻1. The findings provide crucial insights into the relationship between the assembly and performance of AuNCs and ZIFs, offering guidance for designing ultrasensitive multifunctional biosensors applicable in the field of biosensing. |
| format | Article |
| id | doaj-art-14b3a78e772648b086e796fc31c21d04 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-14b3a78e772648b086e796fc31c21d042025-01-29T09:50:19ZengWileyAdvanced Science2198-38442025-01-01124n/an/a10.1002/advs.202408400Accurately Tunable AuNC‐ZIF Content Architecture Based on Coordination‐Dissociation Mechanism Enables Highly Brightness Dual‐Site Fluorescent BiosensorJunyang Chen0Yuqian Wang1Runpu Shen2Wei Li3Sainan Gao4Zhikang Xiao5Qiyan Lv6Xiaojie Song7Jianzhong Xu8Gaoxiang Xu9Huifang Cui10Zhaohui Li11School of Life Sciences Zhengzhou University Zhengzhou Henan 450001 ChinaSchool of Life Sciences Zhengzhou University Zhengzhou Henan 450001 ChinaSchool of Chemistry and Chemical Engineering Shaoxing University Shaoxing Zhejiang 312000 ChinaSchool of Basic Medical Sciences Zhengzhou University Zhengzhou Henan 450001 ChinaSchool of Life Sciences Zhengzhou University Zhengzhou Henan 450001 ChinaDepartment of Orthopedics The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan 450052 ChinaSchool of Life Sciences Zhengzhou University Zhengzhou Henan 450001 ChinaSchool of Life Sciences Zhengzhou University Zhengzhou Henan 450001 ChinaSchool of Basic Medical Sciences Zhengzhou University Zhengzhou Henan 450001 ChinaDepartment of Orthopedics The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan 450052 ChinaSchool of Life Sciences Zhengzhou University Zhengzhou Henan 450001 ChinaCollege of Chemistry, Institute of Analytical Chemistry for Life Science Zhengzhou University Zhengzhou Henan 450001 ChinaAbstract The quantum yield and fluorescence intensity of gold nanocluster (AuNC) nanocarriers are critical parameters for developing ultrasensitive biosensors. In this study, AuNCs‐zeolitic‐imidazolate‐framework (Au‐ZIF) nanocomposites are systematically constructed by impregnating AuNCs onto the ZIF‐8 surface through a coordination‐dissociation mechanism, resulting in a dual‐site fluorescence‐loaded structure. In this configuration, AuNCs are anchored to the external surface while the integrity of the inner cavity remains intact. The surface of ZIF‐8 induces a confinement effect on the configuration and electrons of AuNCs, significantly enhancing luminescence (18‐fold increase). The quantum yield of AuNCs exhibits an increase of more than 13‐fold, from 2.80% to 38.1%. This approach demonstrates broad applicability and maintains strong fluorescence across different ZIFs. Additionally, a novel nanocomposite, Au‐ZIF@carbon‐dots (CDs), is synthesized by encapsulating CDs into the inner cavity of Au‐ZIF. A ratiometric fluorescence detection platform is subsequently developed and incorporated into hydrogels for the quantitative detection of the pesticide triazophos. By employing an image‐processing algorithm, quantitative detection is achieved with a detection limit of 0.07 ng mL⁻1. The findings provide crucial insights into the relationship between the assembly and performance of AuNCs and ZIFs, offering guidance for designing ultrasensitive multifunctional biosensors applicable in the field of biosensing.https://doi.org/10.1002/advs.202408400biosensorscarbon dots (CDs)dual‐site fluorescencegold nanoclusterszeolitic imidazolate frameworks (ZIFs) |
| spellingShingle | Junyang Chen Yuqian Wang Runpu Shen Wei Li Sainan Gao Zhikang Xiao Qiyan Lv Xiaojie Song Jianzhong Xu Gaoxiang Xu Huifang Cui Zhaohui Li Accurately Tunable AuNC‐ZIF Content Architecture Based on Coordination‐Dissociation Mechanism Enables Highly Brightness Dual‐Site Fluorescent Biosensor Advanced Science biosensors carbon dots (CDs) dual‐site fluorescence gold nanoclusters zeolitic imidazolate frameworks (ZIFs) |
| title | Accurately Tunable AuNC‐ZIF Content Architecture Based on Coordination‐Dissociation Mechanism Enables Highly Brightness Dual‐Site Fluorescent Biosensor |
| title_full | Accurately Tunable AuNC‐ZIF Content Architecture Based on Coordination‐Dissociation Mechanism Enables Highly Brightness Dual‐Site Fluorescent Biosensor |
| title_fullStr | Accurately Tunable AuNC‐ZIF Content Architecture Based on Coordination‐Dissociation Mechanism Enables Highly Brightness Dual‐Site Fluorescent Biosensor |
| title_full_unstemmed | Accurately Tunable AuNC‐ZIF Content Architecture Based on Coordination‐Dissociation Mechanism Enables Highly Brightness Dual‐Site Fluorescent Biosensor |
| title_short | Accurately Tunable AuNC‐ZIF Content Architecture Based on Coordination‐Dissociation Mechanism Enables Highly Brightness Dual‐Site Fluorescent Biosensor |
| title_sort | accurately tunable aunc zif content architecture based on coordination dissociation mechanism enables highly brightness dual site fluorescent biosensor |
| topic | biosensors carbon dots (CDs) dual‐site fluorescence gold nanoclusters zeolitic imidazolate frameworks (ZIFs) |
| url | https://doi.org/10.1002/advs.202408400 |
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