Diagnostic efficacy of Brucella abortus strain RB51 in experimentally inoculated Sprague-Dawley rats using western blot assay
Background: To investigate the diagnosis and efficacy of Brucella abortus strain RB51 (SRB51) in experimentally inoculated Sprague-Dawley (SD) rat using western blot assay. Methodology: Female SD rats were orally administered with 1.0 x 107 colony forming unit (cfu) suspension of SRB51 and half of t...
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The Journal of Infection in Developing Countries
2008-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/202 |
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| author | Siddiqur Rahman Byeong Kirl Baek |
| author_facet | Siddiqur Rahman Byeong Kirl Baek |
| author_sort | Siddiqur Rahman |
| collection | DOAJ |
| description | Background: To investigate the diagnosis and efficacy of Brucella abortus strain RB51 (SRB51) in experimentally inoculated Sprague-Dawley (SD) rat using western blot assay.
Methodology: Female SD rats were orally administered with 1.0 x 107 colony forming unit (cfu) suspension of SRB51 and half of these SD rats were challenged at 4 weeks post inoculation with 1.0 x 109 cfu suspension of B. abortus biotype 1 isolated in South Korea. Sera of SD rats were monitored at regular intervals by western blot assay using whole cell antigen of B. abortus strain 1119-3 (S1119-3). The bacteriological examination of blood and clinical examination of the rats were also performed.
Results: There were several bands at 120, 70, 45, 30, 20 kDa and clear specific bands were found after vaccination (20, 70 kDa) and challenge (15, 20, 45, 70, 120 kDa). The highest immune response was observed in sera 4 weeks post SRB51 vaccination. SRB51 was recovered from the blood of all of SRB51 inoculated rats until one week post vaccination and there were no clinical signs in that inoculated rats.
Conclusions: It is concluded that the SRB51 elicits antigen specific immunity in SD rats based on western blot assay. |
| format | Article |
| id | doaj-art-14a00a7f5cc74940a03af473612f1d63 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2008-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-14a00a7f5cc74940a03af473612f1d632025-08-20T02:27:18ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802008-10-0120510.3855/jidc.202Diagnostic efficacy of Brucella abortus strain RB51 in experimentally inoculated Sprague-Dawley rats using western blot assaySiddiqur Rahman0Byeong Kirl Baek1Department of Medicine, Bangladesh Agricultural University, MymensinghCollege of Veterinary Medicine, Chonbuk National University, JeonjuBackground: To investigate the diagnosis and efficacy of Brucella abortus strain RB51 (SRB51) in experimentally inoculated Sprague-Dawley (SD) rat using western blot assay. Methodology: Female SD rats were orally administered with 1.0 x 107 colony forming unit (cfu) suspension of SRB51 and half of these SD rats were challenged at 4 weeks post inoculation with 1.0 x 109 cfu suspension of B. abortus biotype 1 isolated in South Korea. Sera of SD rats were monitored at regular intervals by western blot assay using whole cell antigen of B. abortus strain 1119-3 (S1119-3). The bacteriological examination of blood and clinical examination of the rats were also performed. Results: There were several bands at 120, 70, 45, 30, 20 kDa and clear specific bands were found after vaccination (20, 70 kDa) and challenge (15, 20, 45, 70, 120 kDa). The highest immune response was observed in sera 4 weeks post SRB51 vaccination. SRB51 was recovered from the blood of all of SRB51 inoculated rats until one week post vaccination and there were no clinical signs in that inoculated rats. Conclusions: It is concluded that the SRB51 elicits antigen specific immunity in SD rats based on western blot assay.https://jidc.org/index.php/journal/article/view/202B. abortus strain RB51B. abortus biotype 1Sprague-Dawley ratswestern blot assaySouth Korea |
| spellingShingle | Siddiqur Rahman Byeong Kirl Baek Diagnostic efficacy of Brucella abortus strain RB51 in experimentally inoculated Sprague-Dawley rats using western blot assay Journal of Infection in Developing Countries B. abortus strain RB51 B. abortus biotype 1 Sprague-Dawley rats western blot assay South Korea |
| title | Diagnostic efficacy of Brucella abortus strain RB51 in experimentally inoculated Sprague-Dawley rats using western blot assay |
| title_full | Diagnostic efficacy of Brucella abortus strain RB51 in experimentally inoculated Sprague-Dawley rats using western blot assay |
| title_fullStr | Diagnostic efficacy of Brucella abortus strain RB51 in experimentally inoculated Sprague-Dawley rats using western blot assay |
| title_full_unstemmed | Diagnostic efficacy of Brucella abortus strain RB51 in experimentally inoculated Sprague-Dawley rats using western blot assay |
| title_short | Diagnostic efficacy of Brucella abortus strain RB51 in experimentally inoculated Sprague-Dawley rats using western blot assay |
| title_sort | diagnostic efficacy of brucella abortus strain rb51 in experimentally inoculated sprague dawley rats using western blot assay |
| topic | B. abortus strain RB51 B. abortus biotype 1 Sprague-Dawley rats western blot assay South Korea |
| url | https://jidc.org/index.php/journal/article/view/202 |
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