Mitigation of salinity stress effects on Vicia faba L. growth and productivity using proline and salicylic acid foliar application

High soil salinity causes a negative impact on plant growth and lowers crop yields. Thus, pot experiments were conducted to investigate the impact of foliar application of salicylic acid (SA) and proline (Pro), separately and combined, on enhancing salinity tolerance in broad beans. Salinity stress...

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Main Authors: Hossam El-Beltagi, Mohamed El-Nady, Ahmed Mahmoud Ismail, Metwaly Mahfouz Salem Metwaly
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2025-07-01
Series:Plant Protection Science
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Online Access:https://pps.agriculturejournals.cz/artkey/pps-202503-0002_mitigation-of-salinity-stress-effects-on-vicia-faba-l-growth-and-productivity-using-proline-and-salicylic-acid.php
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author Hossam El-Beltagi
Mohamed El-Nady
Ahmed Mahmoud Ismail
Metwaly Mahfouz Salem Metwaly
author_facet Hossam El-Beltagi
Mohamed El-Nady
Ahmed Mahmoud Ismail
Metwaly Mahfouz Salem Metwaly
author_sort Hossam El-Beltagi
collection DOAJ
description High soil salinity causes a negative impact on plant growth and lowers crop yields. Thus, pot experiments were conducted to investigate the impact of foliar application of salicylic acid (SA) and proline (Pro), separately and combined, on enhancing salinity tolerance in broad beans. Salinity stress (4.69 ds/m and 6.25 ds/m) significantly reduced plant growth (plant height, leaf area, number of leaf/plant, plant dry weight), chlorophyll pigment content (chlorophyll a, b or total), relative water content, K/Na ratio, seed yield per plant, and N, P, K, and crude protein content in broad bean seeds. Foliar application of Pro and SA, either individually or in combination, enhanced plant growth parameters, chlorophyll pigment content, endogenous proline levels, phenol content, and the activities of antioxidant enzymes [antioxidant enzymes including catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD)]. Additionally, these treatments enhanced plant seed yield, N, P, K, and crude protein levels in the seeds. The combined foliar application of Pro and SA was more effective in mitigating salinity stress's harmful effects than using either substance alone. These findings indicate that foliar application of SA and Pro, either individually or in combination, alleviated the adverse effects of salinity on broad beans, with the combined application proving to be the most effective.
format Article
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institution Kabale University
issn 1212-2580
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language English
publishDate 2025-07-01
publisher Czech Academy of Agricultural Sciences
record_format Article
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spelling doaj-art-149fe7564ed8404e99ef5fa8e3987bea2025-08-20T03:32:53ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412025-07-0161322224110.17221/127/2024-PPSpps-202503-0002Mitigation of salinity stress effects on Vicia faba L. growth and productivity using proline and salicylic acid foliar applicationHossam El-Beltagi0https://orcid.org/0000-0003-4433-2034Mohamed El-Nady1Ahmed Mahmoud Ismail2Metwaly Mahfouz Salem Metwaly3Agricultural Biotechnology Department, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi ArabiaDepartment of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, EgyptDepartment of Arid Land Agriculture, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi ArabiaDepartment of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, EgyptHigh soil salinity causes a negative impact on plant growth and lowers crop yields. Thus, pot experiments were conducted to investigate the impact of foliar application of salicylic acid (SA) and proline (Pro), separately and combined, on enhancing salinity tolerance in broad beans. Salinity stress (4.69 ds/m and 6.25 ds/m) significantly reduced plant growth (plant height, leaf area, number of leaf/plant, plant dry weight), chlorophyll pigment content (chlorophyll a, b or total), relative water content, K/Na ratio, seed yield per plant, and N, P, K, and crude protein content in broad bean seeds. Foliar application of Pro and SA, either individually or in combination, enhanced plant growth parameters, chlorophyll pigment content, endogenous proline levels, phenol content, and the activities of antioxidant enzymes [antioxidant enzymes including catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD)]. Additionally, these treatments enhanced plant seed yield, N, P, K, and crude protein levels in the seeds. The combined foliar application of Pro and SA was more effective in mitigating salinity stress's harmful effects than using either substance alone. These findings indicate that foliar application of SA and Pro, either individually or in combination, alleviated the adverse effects of salinity on broad beans, with the combined application proving to be the most effective.https://pps.agriculturejournals.cz/artkey/pps-202503-0002_mitigation-of-salinity-stress-effects-on-vicia-faba-l-growth-and-productivity-using-proline-and-salicylic-acid.phpchlorophyll pigmentsendogenous proline contentantioxidant enzymes activityrelative water contentphenols contentmembrane stability
spellingShingle Hossam El-Beltagi
Mohamed El-Nady
Ahmed Mahmoud Ismail
Metwaly Mahfouz Salem Metwaly
Mitigation of salinity stress effects on Vicia faba L. growth and productivity using proline and salicylic acid foliar application
Plant Protection Science
chlorophyll pigments
endogenous proline content
antioxidant enzymes activity
relative water content
phenols content
membrane stability
title Mitigation of salinity stress effects on Vicia faba L. growth and productivity using proline and salicylic acid foliar application
title_full Mitigation of salinity stress effects on Vicia faba L. growth and productivity using proline and salicylic acid foliar application
title_fullStr Mitigation of salinity stress effects on Vicia faba L. growth and productivity using proline and salicylic acid foliar application
title_full_unstemmed Mitigation of salinity stress effects on Vicia faba L. growth and productivity using proline and salicylic acid foliar application
title_short Mitigation of salinity stress effects on Vicia faba L. growth and productivity using proline and salicylic acid foliar application
title_sort mitigation of salinity stress effects on vicia faba l growth and productivity using proline and salicylic acid foliar application
topic chlorophyll pigments
endogenous proline content
antioxidant enzymes activity
relative water content
phenols content
membrane stability
url https://pps.agriculturejournals.cz/artkey/pps-202503-0002_mitigation-of-salinity-stress-effects-on-vicia-faba-l-growth-and-productivity-using-proline-and-salicylic-acid.php
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AT ahmedmahmoudismail mitigationofsalinitystresseffectsonviciafabalgrowthandproductivityusingprolineandsalicylicacidfoliarapplication
AT metwalymahfouzsalemmetwaly mitigationofsalinitystresseffectsonviciafabalgrowthandproductivityusingprolineandsalicylicacidfoliarapplication