Abcb1 is involved in the efflux of trivalent inorganic arsenic from brain microvascular endothelial cells
Arsenic (As) can penetrate brain tissue through the blood-brain barrier (BBB), and the ATP-binding cassette subfamily B member 1 (Abcb1) has been shown to facilitate the transport of inorganic arsenic (iAs) in animal liver, small intestine, and yeast. However, the relationship between Abcb1 and BBB...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015914 |
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| author | Man Lv Ziqiao Guan Jia Cui Xinbo Ma Kunyu Zhang Xinhua Shao Meichen Zhang Yanhui Gao Yanmei Yang Xiaona Liu |
| author_facet | Man Lv Ziqiao Guan Jia Cui Xinbo Ma Kunyu Zhang Xinhua Shao Meichen Zhang Yanhui Gao Yanmei Yang Xiaona Liu |
| author_sort | Man Lv |
| collection | DOAJ |
| description | Arsenic (As) can penetrate brain tissue through the blood-brain barrier (BBB), and the ATP-binding cassette subfamily B member 1 (Abcb1) has been shown to facilitate the transport of inorganic arsenic (iAs) in animal liver, small intestine, and yeast. However, the relationship between Abcb1 and BBB has not been reported, and the mechanism of brain microvascular endothelial cells Abcb1 on the transport of iAs needs to be further studied. Increased arsenic levels were observed in mice exposed to 25 mg/L or 50 mg/L of sodium arsenite (NaAsO2) in drinking water, and both arsenic uptake and efflux were detected in bEnd.3 cells treated with 16 μmol/L NaAsO2. Elevated levels of Abcb1 protein were found in the NaAsO2-exposed mouse brain microvascular endothelium and in NaAsO2-treated bEnd.3 cells. Inhibition of Abcb1’s efflux function significantly reduced the 2-hour arsenic efflux rate in bEnd.3 cells loaded arsenic. Conversely, overexpression of either Abcb1a or Abcb1b significantly increased the 2-hour arsenic efflux rate in these cells loaded arsenic. These findings suggest that Abcb1 may play a crucial role in mediating arsenic efflux from mouse brain microvascular endothelial cells. |
| format | Article |
| id | doaj-art-1499a687fedc47f2a1293642a7fc3396 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-1499a687fedc47f2a1293642a7fc33962025-01-23T05:25:50ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117515Abcb1 is involved in the efflux of trivalent inorganic arsenic from brain microvascular endothelial cellsMan Lv0Ziqiao Guan1Jia Cui2Xinbo Ma3Kunyu Zhang4Xinhua Shao5Meichen Zhang6Yanhui Gao7Yanmei Yang8Xiaona Liu9Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health of P. R. China, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, ChinaCenter for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health of P. R. China, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, ChinaCenter for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health of P. R. China, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, ChinaCenter for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health of P. R. China, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, ChinaCenter for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health of P. R. China, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, ChinaCenter for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health of P. R. China, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, ChinaCenter for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health of P. R. China, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, ChinaCenter for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health of P. R. China, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, ChinaCenter for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health of P. R. China, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China; Corresponding authors at: Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China.Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health of P. R. China, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China; Corresponding authors at: Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Baojian Road, Harbin, Heilongjiang 150081, China.Arsenic (As) can penetrate brain tissue through the blood-brain barrier (BBB), and the ATP-binding cassette subfamily B member 1 (Abcb1) has been shown to facilitate the transport of inorganic arsenic (iAs) in animal liver, small intestine, and yeast. However, the relationship between Abcb1 and BBB has not been reported, and the mechanism of brain microvascular endothelial cells Abcb1 on the transport of iAs needs to be further studied. Increased arsenic levels were observed in mice exposed to 25 mg/L or 50 mg/L of sodium arsenite (NaAsO2) in drinking water, and both arsenic uptake and efflux were detected in bEnd.3 cells treated with 16 μmol/L NaAsO2. Elevated levels of Abcb1 protein were found in the NaAsO2-exposed mouse brain microvascular endothelium and in NaAsO2-treated bEnd.3 cells. Inhibition of Abcb1’s efflux function significantly reduced the 2-hour arsenic efflux rate in bEnd.3 cells loaded arsenic. Conversely, overexpression of either Abcb1a or Abcb1b significantly increased the 2-hour arsenic efflux rate in these cells loaded arsenic. These findings suggest that Abcb1 may play a crucial role in mediating arsenic efflux from mouse brain microvascular endothelial cells.http://www.sciencedirect.com/science/article/pii/S0147651324015914ArsenicBrain microvascular endothelial cellsAbcb1Efflux |
| spellingShingle | Man Lv Ziqiao Guan Jia Cui Xinbo Ma Kunyu Zhang Xinhua Shao Meichen Zhang Yanhui Gao Yanmei Yang Xiaona Liu Abcb1 is involved in the efflux of trivalent inorganic arsenic from brain microvascular endothelial cells Ecotoxicology and Environmental Safety Arsenic Brain microvascular endothelial cells Abcb1 Efflux |
| title | Abcb1 is involved in the efflux of trivalent inorganic arsenic from brain microvascular endothelial cells |
| title_full | Abcb1 is involved in the efflux of trivalent inorganic arsenic from brain microvascular endothelial cells |
| title_fullStr | Abcb1 is involved in the efflux of trivalent inorganic arsenic from brain microvascular endothelial cells |
| title_full_unstemmed | Abcb1 is involved in the efflux of trivalent inorganic arsenic from brain microvascular endothelial cells |
| title_short | Abcb1 is involved in the efflux of trivalent inorganic arsenic from brain microvascular endothelial cells |
| title_sort | abcb1 is involved in the efflux of trivalent inorganic arsenic from brain microvascular endothelial cells |
| topic | Arsenic Brain microvascular endothelial cells Abcb1 Efflux |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015914 |
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