Structural characterization and alleviation on osteoporotic of polysaccharide purified from Psidium guajava L. leaves
Psidium guajava L. leaves contain various bioactive components have been utilized in traditional medicine for the treatment of diseases. A novel acidic polysaccharide (GLP) was extracted and purified from Psidium guajava L. leaves, an underutilized by-product of guava processing. GLP. The molecular...
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Elsevier
2025-08-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154325004764 |
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| author | Meng Meng Rui Zhang Fang Ning Ying Sun Yinyin Feng Xiaoyang He Ran Han Qinghua Liu Huiqing Sun |
| author_facet | Meng Meng Rui Zhang Fang Ning Ying Sun Yinyin Feng Xiaoyang He Ran Han Qinghua Liu Huiqing Sun |
| author_sort | Meng Meng |
| collection | DOAJ |
| description | Psidium guajava L. leaves contain various bioactive components have been utilized in traditional medicine for the treatment of diseases. A novel acidic polysaccharide (GLP) was extracted and purified from Psidium guajava L. leaves, an underutilized by-product of guava processing. GLP. The molecular weight of GLP was found to be 1.723 × 103 kDa and mainly consists of arabinose, galactose, galacturonic acid and rhamnose using high performance liquid chromatography, ion chromatography, methylation analysis and nuclear magnetic resonance techniques. Key glycosidic linkages such as →3)-β-D-Galp-(1→, →3,4)-β-D-GalpA-(1→, and →3)-α-L-Araf-(1 → . were identified. In a retinoic acid-induced mouse model of osteoporosis, GLP administration significantly improved trabecular bone microarchitecture by increasing bone volume fraction, trabecular number, and thickness, while decreasing trabecular separation. Additionally, GLP markedly reduced serum levels of bone turnover markers (ALP, OC, TRACP) and pro-inflammatory cytokines (IL-6, IL-2, TNF-α). Furthermore, GLP modulated gut microbiota composition, enhancing the relative abundance of beneficial genera such as Lachnospira, Oscillospiraceae, and Dubosiella. These findings suggest that GLP not only modulates bone metabolism and inflammation but also may serve as an innovative natural therapeutic agent or functional food ingredient for the prevention and management of osteoporosis, highlighting the potential of valorizing guava leaf by-products in industrial applications. |
| format | Article |
| id | doaj-art-14981608d1d746b9ac64caa28e1f0a7c |
| institution | Kabale University |
| issn | 2666-1543 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-14981608d1d746b9ac64caa28e1f0a7c2025-08-20T03:56:04ZengElsevierJournal of Agriculture and Food Research2666-15432025-08-012210210510.1016/j.jafr.2025.102105Structural characterization and alleviation on osteoporotic of polysaccharide purified from Psidium guajava L. leavesMeng Meng0Rui Zhang1Fang Ning2Ying Sun3Yinyin Feng4Xiaoyang He5Ran Han6Qinghua Liu7Huiqing Sun8State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin Economy Technological Development Area, No. 29, 13th Avenue, Tianjin, 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin Economy Technological Development Area, No. 29, 13th Avenue, Tianjin, 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin Economy Technological Development Area, No. 29, 13th Avenue, Tianjin, 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin Economy Technological Development Area, No. 29, 13th Avenue, Tianjin, 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin Economy Technological Development Area, No. 29, 13th Avenue, Tianjin, 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin Economy Technological Development Area, No. 29, 13th Avenue, Tianjin, 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin Economy Technological Development Area, No. 29, 13th Avenue, Tianjin, 300457, ChinaThe Department of Clinical Laboratory, The Second Affiliated Hospital of Shandong First Medical University, No. 366 Taishan Road, Taian 271000, ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin Economy Technological Development Area, No. 29, 13th Avenue, Tianjin, 300457, China; Corresponding author.Psidium guajava L. leaves contain various bioactive components have been utilized in traditional medicine for the treatment of diseases. A novel acidic polysaccharide (GLP) was extracted and purified from Psidium guajava L. leaves, an underutilized by-product of guava processing. GLP. The molecular weight of GLP was found to be 1.723 × 103 kDa and mainly consists of arabinose, galactose, galacturonic acid and rhamnose using high performance liquid chromatography, ion chromatography, methylation analysis and nuclear magnetic resonance techniques. Key glycosidic linkages such as →3)-β-D-Galp-(1→, →3,4)-β-D-GalpA-(1→, and →3)-α-L-Araf-(1 → . were identified. In a retinoic acid-induced mouse model of osteoporosis, GLP administration significantly improved trabecular bone microarchitecture by increasing bone volume fraction, trabecular number, and thickness, while decreasing trabecular separation. Additionally, GLP markedly reduced serum levels of bone turnover markers (ALP, OC, TRACP) and pro-inflammatory cytokines (IL-6, IL-2, TNF-α). Furthermore, GLP modulated gut microbiota composition, enhancing the relative abundance of beneficial genera such as Lachnospira, Oscillospiraceae, and Dubosiella. These findings suggest that GLP not only modulates bone metabolism and inflammation but also may serve as an innovative natural therapeutic agent or functional food ingredient for the prevention and management of osteoporosis, highlighting the potential of valorizing guava leaf by-products in industrial applications.http://www.sciencedirect.com/science/article/pii/S2666154325004764Psidium guajava L. leavesPolysaccharidesStructureOsteoporosis |
| spellingShingle | Meng Meng Rui Zhang Fang Ning Ying Sun Yinyin Feng Xiaoyang He Ran Han Qinghua Liu Huiqing Sun Structural characterization and alleviation on osteoporotic of polysaccharide purified from Psidium guajava L. leaves Journal of Agriculture and Food Research Psidium guajava L. leaves Polysaccharides Structure Osteoporosis |
| title | Structural characterization and alleviation on osteoporotic of polysaccharide purified from Psidium guajava L. leaves |
| title_full | Structural characterization and alleviation on osteoporotic of polysaccharide purified from Psidium guajava L. leaves |
| title_fullStr | Structural characterization and alleviation on osteoporotic of polysaccharide purified from Psidium guajava L. leaves |
| title_full_unstemmed | Structural characterization and alleviation on osteoporotic of polysaccharide purified from Psidium guajava L. leaves |
| title_short | Structural characterization and alleviation on osteoporotic of polysaccharide purified from Psidium guajava L. leaves |
| title_sort | structural characterization and alleviation on osteoporotic of polysaccharide purified from psidium guajava l leaves |
| topic | Psidium guajava L. leaves Polysaccharides Structure Osteoporosis |
| url | http://www.sciencedirect.com/science/article/pii/S2666154325004764 |
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