A modular platform for bioluminescent RNA tracking
Abstract A complete understanding of RNA biology requires methods for tracking transcripts in vivo. Common strategies rely on fluorogenic probes that are limited in sensitivity, dynamic range, and depth of interrogation, owing to their need for excitation light and tissue autofluorescence. To overco...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-54263-5 |
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| author | Lila P. Halbers Kyle H. Cole Kevin K. Ng Erin B. Fuller Christelle E. T. Chan Chelsea Callicoatte Mariajose Metcalfe Claire C. Chen Ahfnan A. Barhoosh Edison Reid-McLaughlin Alexandra D. Kent Zachary R. Torrey Oswald Steward Andrej Lupták Jennifer A. Prescher |
| author_facet | Lila P. Halbers Kyle H. Cole Kevin K. Ng Erin B. Fuller Christelle E. T. Chan Chelsea Callicoatte Mariajose Metcalfe Claire C. Chen Ahfnan A. Barhoosh Edison Reid-McLaughlin Alexandra D. Kent Zachary R. Torrey Oswald Steward Andrej Lupták Jennifer A. Prescher |
| author_sort | Lila P. Halbers |
| collection | DOAJ |
| description | Abstract A complete understanding of RNA biology requires methods for tracking transcripts in vivo. Common strategies rely on fluorogenic probes that are limited in sensitivity, dynamic range, and depth of interrogation, owing to their need for excitation light and tissue autofluorescence. To overcome these challenges, we report a bioluminescent platform for serial imaging of RNAs. The RNA tags are engineered to recruit light-emitting luciferase fragments (termed RNA lanterns) upon transcription. Robust photon production is observed for RNA targets both in cells and in live animals. Importantly, only a single copy of the tag is necessary for sensitive detection, in sharp contrast to fluorescent platforms requiring multiple repeats. Overall, this work provides a foundational platform for visualizing RNA dynamics from the micro to the macro scale. |
| format | Article |
| id | doaj-art-14957cc7b6ef4cfaba2c4a0e3ebee9ec |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-14957cc7b6ef4cfaba2c4a0e3ebee9ec2025-08-20T02:33:31ZengNature PortfolioNature Communications2041-17232024-11-0115111210.1038/s41467-024-54263-5A modular platform for bioluminescent RNA trackingLila P. Halbers0Kyle H. Cole1Kevin K. Ng2Erin B. Fuller3Christelle E. T. Chan4Chelsea Callicoatte5Mariajose Metcalfe6Claire C. Chen7Ahfnan A. Barhoosh8Edison Reid-McLaughlin9Alexandra D. Kent10Zachary R. Torrey11Oswald Steward12Andrej Lupták13Jennifer A. Prescher14Department of Pharmaceutical Sciences, University of California, IrvineDepartment of Molecular Biology and Biochemistry, University of California, IrvineDepartment of Pharmaceutical Sciences, University of California, IrvineDepartment of Chemistry, University of California, IrvineDepartment of Pharmaceutical Sciences, University of California, IrvineDepartment of Anatomy and Neurobiology, University of California, IrvineDepartment of Anatomy and Neurobiology, University of California, IrvineDepartment of Pharmaceutical Sciences, University of California, IrvineDepartment of Pharmaceutical Sciences, University of California, IrvineDepartment of Chemistry, University of California, IrvineDepartment of Chemistry, University of California, IrvineDepartment of Chemistry, University of California, IrvineDepartment of Anatomy and Neurobiology, University of California, IrvineDepartment of Pharmaceutical Sciences, University of California, IrvineDepartment of Pharmaceutical Sciences, University of California, IrvineAbstract A complete understanding of RNA biology requires methods for tracking transcripts in vivo. Common strategies rely on fluorogenic probes that are limited in sensitivity, dynamic range, and depth of interrogation, owing to their need for excitation light and tissue autofluorescence. To overcome these challenges, we report a bioluminescent platform for serial imaging of RNAs. The RNA tags are engineered to recruit light-emitting luciferase fragments (termed RNA lanterns) upon transcription. Robust photon production is observed for RNA targets both in cells and in live animals. Importantly, only a single copy of the tag is necessary for sensitive detection, in sharp contrast to fluorescent platforms requiring multiple repeats. Overall, this work provides a foundational platform for visualizing RNA dynamics from the micro to the macro scale.https://doi.org/10.1038/s41467-024-54263-5 |
| spellingShingle | Lila P. Halbers Kyle H. Cole Kevin K. Ng Erin B. Fuller Christelle E. T. Chan Chelsea Callicoatte Mariajose Metcalfe Claire C. Chen Ahfnan A. Barhoosh Edison Reid-McLaughlin Alexandra D. Kent Zachary R. Torrey Oswald Steward Andrej Lupták Jennifer A. Prescher A modular platform for bioluminescent RNA tracking Nature Communications |
| title | A modular platform for bioluminescent RNA tracking |
| title_full | A modular platform for bioluminescent RNA tracking |
| title_fullStr | A modular platform for bioluminescent RNA tracking |
| title_full_unstemmed | A modular platform for bioluminescent RNA tracking |
| title_short | A modular platform for bioluminescent RNA tracking |
| title_sort | modular platform for bioluminescent rna tracking |
| url | https://doi.org/10.1038/s41467-024-54263-5 |
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