Chick chorioallantoic membrane model as a preclinical platform for cryoablation studies
Abstract Background The chick chorioallantoic membrane (CAM) model has been utilized for radiofrequency ablation and electroporation, but not yet for cryoablation. This study aims to evaluate the feasibility of the CAM model for preclinical cryoablation research. Methods Two cryoablation protocols w...
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SpringerOpen
2025-05-01
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| Series: | European Radiology Experimental |
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| Online Access: | https://doi.org/10.1186/s41747-025-00592-z |
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| author | Michael Scheschenja Jarmila Jedelská Eva Juchems Marc Weinmann Axel Pagenstecher Frederik Helmprobst Malte Buchholz Marina Tatura Jens Schaefer Udo Bakowsky Alexander M. König Andreas H. Mahnken |
| author_facet | Michael Scheschenja Jarmila Jedelská Eva Juchems Marc Weinmann Axel Pagenstecher Frederik Helmprobst Malte Buchholz Marina Tatura Jens Schaefer Udo Bakowsky Alexander M. König Andreas H. Mahnken |
| author_sort | Michael Scheschenja |
| collection | DOAJ |
| description | Abstract Background The chick chorioallantoic membrane (CAM) model has been utilized for radiofrequency ablation and electroporation, but not yet for cryoablation. This study aims to evaluate the feasibility of the CAM model for preclinical cryoablation research. Methods Two cryoablation protocols were established for the study: 120 s-freeze-120 s-thaw-120 s freeze (120 s protocol) and 180 s-freeze-120 s-thaw-180 s freeze (180 s protocol). The study was divided into two parts. First, to evaluate embryo survival, fertilized chicken eggs were incubated. On embryonic day (ED) 12, cryoablation on CAM was performed according to the two protocols. During cryoablation, the temperature of the CAM was recorded using a thermal camera. Embryo survival was monitored until ED 14. Second, to evaluate tumor cryoablation, human neuroendocrine tumor cells (BON-1) were xenografted onto the CAM of fertilized chicken eggs at ED 8. Cryoablation of the xenografted tumors was then performed on ED 12 according to the two protocols. Ablation outcomes were evaluated by stereomicroscopic and histological assessments after harvesting on ED 14. Results Embryo survival rates were 8/9 in both protocols. A decrease in the peripheral temperature of 4.5 (± 0.9) °C and 6.7 (± 1.0) °C was observed in the 120 s and 180 s protocols, respectively. Complete ablation of CAM-grown tumors was observed in 2/6 (120 s protocol) and 2/5 (180 s protocol) cases, few scattered tumor cells remaining in 2/6 (120 s protocol) and 2/5 (180 s protocol) cases. Residual interconnected tumor cells were visible in 2/6 (120 s protocol) and 1/5 (180 s protocol) cases. Conclusion The CAM model is a feasible platform for preclinical cryoablation studies. Relevance statement Chorioallantoic membrane model is a suitable platform for preclinical cryoablation research. Key Points Chick embryos tolerate the temperature drop during cryoablation well with high survival. Effectiveness of cryoablation on xenografted tumors can be histologically evaluated. Cryoablation protocols for xenografted tumors can be further optimized. Graphical Abstract |
| format | Article |
| id | doaj-art-148c1513af2d41ec8e84bbd1ad9a0103 |
| institution | DOAJ |
| issn | 2509-9280 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | SpringerOpen |
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| series | European Radiology Experimental |
| spelling | doaj-art-148c1513af2d41ec8e84bbd1ad9a01032025-08-20T03:22:04ZengSpringerOpenEuropean Radiology Experimental2509-92802025-05-01911910.1186/s41747-025-00592-zChick chorioallantoic membrane model as a preclinical platform for cryoablation studiesMichael Scheschenja0Jarmila Jedelská1Eva Juchems2Marc Weinmann3Axel Pagenstecher4Frederik Helmprobst5Malte Buchholz6Marina Tatura7Jens Schaefer8Udo Bakowsky9Alexander M. König10Andreas H. Mahnken11Department of Diagnostic and Interventional Radiology, University Hospital Marburg, Philipps-University MarburgDepartment of Diagnostic and Interventional Radiology, University Hospital Marburg, Philipps-University MarburgDepartment of Diagnostic and Interventional Radiology, University Hospital Marburg, Philipps-University MarburgDepartment of Diagnostic and Interventional Radiology, University Hospital Marburg, Philipps-University MarburgMouse Pathology and Electron Microscopy—Core Facility, Institute of Neuropathology, Philipps-University MarburgMouse Pathology and Electron Microscopy—Core Facility, Institute of Neuropathology, Philipps-University MarburgDepartment of Gastroenterology, Endocrinology, Metabolism and Infectiology, Philipps-University MarburgDepartment of Gastroenterology, Endocrinology, Metabolism and Infectiology, Philipps-University MarburgDepartment of Pharmaceutics and Biopharmaceutics, Philipps-University MarburgDepartment of Pharmaceutics and Biopharmaceutics, Philipps-University MarburgDepartment of Diagnostic and Interventional Radiology, University Hospital Marburg, Philipps-University MarburgDepartment of Diagnostic and Interventional Radiology, University Hospital Marburg, Philipps-University MarburgAbstract Background The chick chorioallantoic membrane (CAM) model has been utilized for radiofrequency ablation and electroporation, but not yet for cryoablation. This study aims to evaluate the feasibility of the CAM model for preclinical cryoablation research. Methods Two cryoablation protocols were established for the study: 120 s-freeze-120 s-thaw-120 s freeze (120 s protocol) and 180 s-freeze-120 s-thaw-180 s freeze (180 s protocol). The study was divided into two parts. First, to evaluate embryo survival, fertilized chicken eggs were incubated. On embryonic day (ED) 12, cryoablation on CAM was performed according to the two protocols. During cryoablation, the temperature of the CAM was recorded using a thermal camera. Embryo survival was monitored until ED 14. Second, to evaluate tumor cryoablation, human neuroendocrine tumor cells (BON-1) were xenografted onto the CAM of fertilized chicken eggs at ED 8. Cryoablation of the xenografted tumors was then performed on ED 12 according to the two protocols. Ablation outcomes were evaluated by stereomicroscopic and histological assessments after harvesting on ED 14. Results Embryo survival rates were 8/9 in both protocols. A decrease in the peripheral temperature of 4.5 (± 0.9) °C and 6.7 (± 1.0) °C was observed in the 120 s and 180 s protocols, respectively. Complete ablation of CAM-grown tumors was observed in 2/6 (120 s protocol) and 2/5 (180 s protocol) cases, few scattered tumor cells remaining in 2/6 (120 s protocol) and 2/5 (180 s protocol) cases. Residual interconnected tumor cells were visible in 2/6 (120 s protocol) and 1/5 (180 s protocol) cases. Conclusion The CAM model is a feasible platform for preclinical cryoablation studies. Relevance statement Chorioallantoic membrane model is a suitable platform for preclinical cryoablation research. Key Points Chick embryos tolerate the temperature drop during cryoablation well with high survival. Effectiveness of cryoablation on xenografted tumors can be histologically evaluated. Cryoablation protocols for xenografted tumors can be further optimized. Graphical Abstracthttps://doi.org/10.1186/s41747-025-00592-zChick embryoChorioallantoic membraneCryoablationRadiology (interventional)Xenografts |
| spellingShingle | Michael Scheschenja Jarmila Jedelská Eva Juchems Marc Weinmann Axel Pagenstecher Frederik Helmprobst Malte Buchholz Marina Tatura Jens Schaefer Udo Bakowsky Alexander M. König Andreas H. Mahnken Chick chorioallantoic membrane model as a preclinical platform for cryoablation studies European Radiology Experimental Chick embryo Chorioallantoic membrane Cryoablation Radiology (interventional) Xenografts |
| title | Chick chorioallantoic membrane model as a preclinical platform for cryoablation studies |
| title_full | Chick chorioallantoic membrane model as a preclinical platform for cryoablation studies |
| title_fullStr | Chick chorioallantoic membrane model as a preclinical platform for cryoablation studies |
| title_full_unstemmed | Chick chorioallantoic membrane model as a preclinical platform for cryoablation studies |
| title_short | Chick chorioallantoic membrane model as a preclinical platform for cryoablation studies |
| title_sort | chick chorioallantoic membrane model as a preclinical platform for cryoablation studies |
| topic | Chick embryo Chorioallantoic membrane Cryoablation Radiology (interventional) Xenografts |
| url | https://doi.org/10.1186/s41747-025-00592-z |
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