The study on the design and regulatory effects of synthetic communities of endophytic actinomycetes of Salvia miltiorrhiza Bunge
Abstract Background Microorganisms can confer or enhance various ecological adaptation functions of host plants, offering wide-ranging applications in the cultivation and production of medicinal plants. While root-associated beneficial actinomycetes are commonly used in biological control, their eff...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-08-01
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| Series: | Chemical and Biological Technologies in Agriculture |
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| Online Access: | https://doi.org/10.1186/s40538-025-00815-w |
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| author | Changwen Zheng Wencheng Zhao Yurui Wu Hongmei Jia Guiqi Han Dongmei He Hai Wang Zhuyun Yan |
| author_facet | Changwen Zheng Wencheng Zhao Yurui Wu Hongmei Jia Guiqi Han Dongmei He Hai Wang Zhuyun Yan |
| author_sort | Changwen Zheng |
| collection | DOAJ |
| description | Abstract Background Microorganisms can confer or enhance various ecological adaptation functions of host plants, offering wide-ranging applications in the cultivation and production of medicinal plants. While root-associated beneficial actinomycetes are commonly used in biological control, their effects on plant growth and development have been rarely reported. This study focuses on a total of 13 beneficial endophytic actinomycetes in the roots of Salvia miltiorrhiza Bunge. Under different synthetic community (SynCom) construction principles, we constructed five synthetic communities based on functions and relationships to investigate their effects on growth, nitrogen metabolism, phosphorus metabolism, iron metabolism, resistance, and active components of S. miltiorrhiza. Results Synthetic communities 3 and 4 composed of functionally complementary strains promoted growth more effectively, synthetic communities 1 and 3 composed of distantly related strains increased total phenolic acid content, and synthetic community 5 that composed solely of indole-3-acetic acid (IAA)-producing strains not only promoted growth but also enhanced the active component content. Genome analysis revealed that synthetic communities with terpenes biosynthetic gene clusters promoted S. miltiorrhiza growth. Conclusions Our results suggested synthetic community design should consider strain relationships, plant growth-promoting (PGP) traits, and functional genes. This study introduces novel insights into synthetic community design. Graphical Abstract |
| format | Article |
| id | doaj-art-14882291f8a84beeae0c2bd7701ebae7 |
| institution | DOAJ |
| issn | 2196-5641 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj-art-14882291f8a84beeae0c2bd7701ebae72025-08-20T03:04:26ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412025-08-0112111310.1186/s40538-025-00815-wThe study on the design and regulatory effects of synthetic communities of endophytic actinomycetes of Salvia miltiorrhiza BungeChangwen Zheng0Wencheng Zhao1Yurui Wu2Hongmei Jia3Guiqi Han4Dongmei He5Hai Wang6Zhuyun Yan7School of Pharmacy, Chengdu University of Traditional Chinese MedicineSchool of Pharmacy, Chengdu University of Traditional Chinese MedicineLuoyang County Maternal and Child Health HospitalSchool of Pharmacy, Chengdu University of Traditional Chinese MedicineSchool of Pharmacy, Chengdu University of Traditional Chinese MedicineSchool of Pharmacy, Chengdu University of Traditional Chinese MedicineCollege of Medical Technology, Chengdu University of Traditional Chinese MedicineSchool of Pharmacy, Chengdu University of Traditional Chinese MedicineAbstract Background Microorganisms can confer or enhance various ecological adaptation functions of host plants, offering wide-ranging applications in the cultivation and production of medicinal plants. While root-associated beneficial actinomycetes are commonly used in biological control, their effects on plant growth and development have been rarely reported. This study focuses on a total of 13 beneficial endophytic actinomycetes in the roots of Salvia miltiorrhiza Bunge. Under different synthetic community (SynCom) construction principles, we constructed five synthetic communities based on functions and relationships to investigate their effects on growth, nitrogen metabolism, phosphorus metabolism, iron metabolism, resistance, and active components of S. miltiorrhiza. Results Synthetic communities 3 and 4 composed of functionally complementary strains promoted growth more effectively, synthetic communities 1 and 3 composed of distantly related strains increased total phenolic acid content, and synthetic community 5 that composed solely of indole-3-acetic acid (IAA)-producing strains not only promoted growth but also enhanced the active component content. Genome analysis revealed that synthetic communities with terpenes biosynthetic gene clusters promoted S. miltiorrhiza growth. Conclusions Our results suggested synthetic community design should consider strain relationships, plant growth-promoting (PGP) traits, and functional genes. This study introduces novel insights into synthetic community design. Graphical Abstracthttps://doi.org/10.1186/s40538-025-00815-wSalvia miltiorrhiza BungeActinomycetesSynthetic communityGrowth-promoting effectGrowth-promoting mechanism |
| spellingShingle | Changwen Zheng Wencheng Zhao Yurui Wu Hongmei Jia Guiqi Han Dongmei He Hai Wang Zhuyun Yan The study on the design and regulatory effects of synthetic communities of endophytic actinomycetes of Salvia miltiorrhiza Bunge Chemical and Biological Technologies in Agriculture Salvia miltiorrhiza Bunge Actinomycetes Synthetic community Growth-promoting effect Growth-promoting mechanism |
| title | The study on the design and regulatory effects of synthetic communities of endophytic actinomycetes of Salvia miltiorrhiza Bunge |
| title_full | The study on the design and regulatory effects of synthetic communities of endophytic actinomycetes of Salvia miltiorrhiza Bunge |
| title_fullStr | The study on the design and regulatory effects of synthetic communities of endophytic actinomycetes of Salvia miltiorrhiza Bunge |
| title_full_unstemmed | The study on the design and regulatory effects of synthetic communities of endophytic actinomycetes of Salvia miltiorrhiza Bunge |
| title_short | The study on the design and regulatory effects of synthetic communities of endophytic actinomycetes of Salvia miltiorrhiza Bunge |
| title_sort | study on the design and regulatory effects of synthetic communities of endophytic actinomycetes of salvia miltiorrhiza bunge |
| topic | Salvia miltiorrhiza Bunge Actinomycetes Synthetic community Growth-promoting effect Growth-promoting mechanism |
| url | https://doi.org/10.1186/s40538-025-00815-w |
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