Incidence of Extended Spectrum β-Lactamase Genes (ESBLs) among community and health care infection in Mansoura University Hospital, Egypt
Abstract Background Multidrug-resistant (MDR) Gram-negative bacteria pose a significant challenge due to their limited treatment options. The production of extended-spectrum β-lactamases (ESBLs) is an important mechanism of resistance. This study aimed to identify the incidence and characteristics o...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-05-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-025-04030-3 |
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| Summary: | Abstract Background Multidrug-resistant (MDR) Gram-negative bacteria pose a significant challenge due to their limited treatment options. The production of extended-spectrum β-lactamases (ESBLs) is an important mechanism of resistance. This study aimed to identify the incidence and characteristics of ESBL-encoding genes (bla CTX-M, bla TEM, bla SHV, and bla OXA) in MDR isolates. Materials and methods A cross-sectional study was conducted from September 2022 to May 2023. ESBL-producing isolates (n = 105) out of 412 were recovered from hospitalized and outpatient settings and analyzed. Standard microbiological methods were used for isolates identification, susceptibility testing, and phenotypic ESBL detection. Additionally, bla CTX-M, bla TEM, bla SHV, and bla OXA genes were identified using conventional PCR. Results Molecular profiling of β-lactamase determinants was conducted via PCR targeting bla CTX-M, bla TEM, bla SHV, and bla OXA genes. Among phenotypically confirmed (100%) ESBL producers, 98% harbored one or more target genes, with bla CTX-M predominant (81%), followed by bla SHV (70.4%), bla TEM (62%), and bla OXA (30.4%). Carbapenem resistance was higher in ESBL-producing strains compared to non-ESBL strains. Extensively drug-resistant (XDR) isolates were the most common across hospital departments and outpatients. Discussion This study highlights the significant prevalence of ESBL genes and multidrug resistance among Gram-negative bacteria. The dominance of bla CTX-M and the existence of multiple resistance genes raise concerns about limited treatment options. The findings emphasize the need for stricter antibiotic stewardship and infection control measures to curb the spread of MDR pathogens. Conclusion This study provides valuable insights into the alarming incidence of ESBL genes and MDR in Mansoura, Egypt. Continuous surveillance and implementation of effective control strategies are crucial to combat this growing public health threat. |
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| ISSN: | 1471-2180 |