Application of high-throughput fully automated air formaldehyde detection technology
BackgroundFormaldehyde is a toxic gas. Automatic iodine analyzer is a high-throughput automatic instrument for measuring water and urine iodine, but at present, there is a lack of its application in the determination for air formaldehyde. ObjectiveTo develop a high-throughput method for air formalde...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Editorial Committee of Journal of Environmental and Occupational Medicine
2024-10-01
|
| Series: | 环境与职业医学 |
| Subjects: | |
| Online Access: | http://www.jeom.org/article/cn/10.11836/JEOM24148 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850193354143301632 |
|---|---|
| author | Xiaoling YANG Junhong LI Zhiyong CHEN Yi JIN |
| author_facet | Xiaoling YANG Junhong LI Zhiyong CHEN Yi JIN |
| author_sort | Xiaoling YANG |
| collection | DOAJ |
| description | BackgroundFormaldehyde is a toxic gas. Automatic iodine analyzer is a high-throughput automatic instrument for measuring water and urine iodine, but at present, there is a lack of its application in the determination for air formaldehyde. ObjectiveTo develop a high-throughput method for air formaldehyde determination with an automated iodine analyzer. MethodsThe formaldehyde in the air was collected with an atmospheric sampler, and 1.0 mL of working curve solution, standard solution, and sample solution were loaded to an automated iodine analyzer. Solution resting time and instrumental parameters, including detection wavelength, sample needle depth, and regent2 (R2) reaction time were optimized. Finally, the method was validated by precision, detection and quantification limits, and spiked recovery. Four different concentrations of standard substance solutions and 10 air samples were collected for comparison between manual measurement method and high-throughput fully automated detection method. ResultsThe final optimized conditions in this study were: 2 h and above resting time for prepared solution, and the optimal parameters of the instrument work were: detection wavelength at 660 nm, sample needle depth at 2800, and R2 reaction time at 900 s. Under the optimized conditions the linear equation of the method was optical density(D) =0.9787 × formaldehyde concentration (C) − 0.0364 and the correlation coefficient was 1.0000. The limits of detection (LOD) and quantification (LOQ) were 0.015 μg·mL−1 and 0.052 μg·mL−1 , respectively. The relative standard deviations (RSDs) of the intra-day precision experiments were 1.35% and 1.18% for the two different concentrations of standard sample solutions. The RSDs of the inter-day precision experiments were 1.82% and 1.50%, respectively. The recoveries ranged from 96.8% to 107.4%. The results produced by spectrophotometry and automated detection method in the comparison study were identical, and the measurements of selected four concentrations of standard solutions were within the uncertainty range. ConclusionThe method established in this study features small solvent dosage, fully automated high throughput, comparable sensitivity to the national standard method, high accuracy, and inter-day and intra-day precision with a recovery rate above 96%. It can provide strong technical support for the determination of air formaldehyde. |
| format | Article |
| id | doaj-art-14787efe0cd149c2b6a2b59c59e86641 |
| institution | OA Journals |
| issn | 2095-9982 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Editorial Committee of Journal of Environmental and Occupational Medicine |
| record_format | Article |
| series | 环境与职业医学 |
| spelling | doaj-art-14787efe0cd149c2b6a2b59c59e866412025-08-20T02:14:19ZengEditorial Committee of Journal of Environmental and Occupational Medicine环境与职业医学2095-99822024-10-0141101186119010.11836/JEOM2414824148Application of high-throughput fully automated air formaldehyde detection technologyXiaoling YANG0Junhong LI1Zhiyong CHEN2Yi JIN3Changzhou Center for Disease Control and Prevention Physical and Chemical LaboratoryChangzhou Center for Disease Control and Prevention Physical and Chemical LaboratoryChangzhou Center for Disease Control and Prevention Food and Environmental Disease Section, Changzhou Center for Disease Control and Prevention, Jiangsu, Changzhou 213001Changzhou Center for Disease Control and Prevention Physical and Chemical LaboratoryBackgroundFormaldehyde is a toxic gas. Automatic iodine analyzer is a high-throughput automatic instrument for measuring water and urine iodine, but at present, there is a lack of its application in the determination for air formaldehyde. ObjectiveTo develop a high-throughput method for air formaldehyde determination with an automated iodine analyzer. MethodsThe formaldehyde in the air was collected with an atmospheric sampler, and 1.0 mL of working curve solution, standard solution, and sample solution were loaded to an automated iodine analyzer. Solution resting time and instrumental parameters, including detection wavelength, sample needle depth, and regent2 (R2) reaction time were optimized. Finally, the method was validated by precision, detection and quantification limits, and spiked recovery. Four different concentrations of standard substance solutions and 10 air samples were collected for comparison between manual measurement method and high-throughput fully automated detection method. ResultsThe final optimized conditions in this study were: 2 h and above resting time for prepared solution, and the optimal parameters of the instrument work were: detection wavelength at 660 nm, sample needle depth at 2800, and R2 reaction time at 900 s. Under the optimized conditions the linear equation of the method was optical density(D) =0.9787 × formaldehyde concentration (C) − 0.0364 and the correlation coefficient was 1.0000. The limits of detection (LOD) and quantification (LOQ) were 0.015 μg·mL−1 and 0.052 μg·mL−1 , respectively. The relative standard deviations (RSDs) of the intra-day precision experiments were 1.35% and 1.18% for the two different concentrations of standard sample solutions. The RSDs of the inter-day precision experiments were 1.82% and 1.50%, respectively. The recoveries ranged from 96.8% to 107.4%. The results produced by spectrophotometry and automated detection method in the comparison study were identical, and the measurements of selected four concentrations of standard solutions were within the uncertainty range. ConclusionThe method established in this study features small solvent dosage, fully automated high throughput, comparable sensitivity to the national standard method, high accuracy, and inter-day and intra-day precision with a recovery rate above 96%. It can provide strong technical support for the determination of air formaldehyde.http://www.jeom.org/article/cn/10.11836/JEOM24148high-throughput fully automated detection technologyambient airformaldehyde |
| spellingShingle | Xiaoling YANG Junhong LI Zhiyong CHEN Yi JIN Application of high-throughput fully automated air formaldehyde detection technology 环境与职业医学 high-throughput fully automated detection technology ambient air formaldehyde |
| title | Application of high-throughput fully automated air formaldehyde detection technology |
| title_full | Application of high-throughput fully automated air formaldehyde detection technology |
| title_fullStr | Application of high-throughput fully automated air formaldehyde detection technology |
| title_full_unstemmed | Application of high-throughput fully automated air formaldehyde detection technology |
| title_short | Application of high-throughput fully automated air formaldehyde detection technology |
| title_sort | application of high throughput fully automated air formaldehyde detection technology |
| topic | high-throughput fully automated detection technology ambient air formaldehyde |
| url | http://www.jeom.org/article/cn/10.11836/JEOM24148 |
| work_keys_str_mv | AT xiaolingyang applicationofhighthroughputfullyautomatedairformaldehydedetectiontechnology AT junhongli applicationofhighthroughputfullyautomatedairformaldehydedetectiontechnology AT zhiyongchen applicationofhighthroughputfullyautomatedairformaldehydedetectiontechnology AT yijin applicationofhighthroughputfullyautomatedairformaldehydedetectiontechnology |