Understanding Collaborative Effects between the Polymer Gel Structure and the Applied Electrical Field in Gel Electrophoresis Separation
The collaborative effects between an applied orthogonal electrical field and the internal structure of polymer gels in gel electrophoresis is studied by using microscopic-based electrophoretic transport models that then are upscaled via the format of electro kinetics-hydrodynamics (EKHD). The interp...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2019/6194674 |
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| _version_ | 1849308257087127552 |
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| author | Jennifer A. Pascal Koteswara Rao Medidhi Mario A. Oyanader Holly A. Stretz Pedro E. Arce |
| author_facet | Jennifer A. Pascal Koteswara Rao Medidhi Mario A. Oyanader Holly A. Stretz Pedro E. Arce |
| author_sort | Jennifer A. Pascal |
| collection | DOAJ |
| description | The collaborative effects between an applied orthogonal electrical field and the internal structure of polymer gels in gel electrophoresis is studied by using microscopic-based electrophoretic transport models that then are upscaled via the format of electro kinetics-hydrodynamics (EKHD). The interplay of the electrical field and internal gel morphology could impact the separation of biomolecules that, because of similar chemical properties, are usually difficult to separate. In this study, we focus on an irregular pore geometry of the polymer-gel structure by using an axially varying pore (i.e., an axially divergent section) and an orthogonal (to the main flow of solutes) applied electrical field. The microscopic-based conservation of species equation is formulated for the standard case of electrophoresis of charged particles within a geometrical domain, i.e., a pore, and upscaled to obtain macroscopic-based diffusion and mobility coefficients. These coefficients are then used in the calculation of the optimal time of separation to study the effect of the varying parameters of the pore structure under different values of the electrical field. The results are qualitatively consistent with those reported, in the literature, by using computational-based approaches as well as with experiments also reported in the literature, previously. The study shows the important collaborative effects between the applied electrical field and the internal geometry of the polymer gels that could lead to improving biomolecule separation in gel electrophoresis. |
| format | Article |
| id | doaj-art-147720f7126f4033bb2eeddd9b3daaa8 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-147720f7126f4033bb2eeddd9b3daaa82025-08-20T03:54:29ZengWileyInternational Journal of Polymer Science1687-94221687-94302019-01-01201910.1155/2019/61946746194674Understanding Collaborative Effects between the Polymer Gel Structure and the Applied Electrical Field in Gel Electrophoresis SeparationJennifer A. Pascal0Koteswara Rao Medidhi1Mario A. Oyanader2Holly A. Stretz3Pedro E. Arce4Department of Chemical Engineering, Tennessee Technological University, Cookeville, TN 38505, USADepartment of Chemical Engineering, Tennessee Technological University, Cookeville, TN 38505, USADepartment of Chemical Engineering, California Baptist University, 8432 Magnolia Ave, Riverside, CA 92504, USADepartment of Chemical Engineering, Tennessee Technological University, Cookeville, TN 38505, USADepartment of Chemical Engineering, Tennessee Technological University, Cookeville, TN 38505, USAThe collaborative effects between an applied orthogonal electrical field and the internal structure of polymer gels in gel electrophoresis is studied by using microscopic-based electrophoretic transport models that then are upscaled via the format of electro kinetics-hydrodynamics (EKHD). The interplay of the electrical field and internal gel morphology could impact the separation of biomolecules that, because of similar chemical properties, are usually difficult to separate. In this study, we focus on an irregular pore geometry of the polymer-gel structure by using an axially varying pore (i.e., an axially divergent section) and an orthogonal (to the main flow of solutes) applied electrical field. The microscopic-based conservation of species equation is formulated for the standard case of electrophoresis of charged particles within a geometrical domain, i.e., a pore, and upscaled to obtain macroscopic-based diffusion and mobility coefficients. These coefficients are then used in the calculation of the optimal time of separation to study the effect of the varying parameters of the pore structure under different values of the electrical field. The results are qualitatively consistent with those reported, in the literature, by using computational-based approaches as well as with experiments also reported in the literature, previously. The study shows the important collaborative effects between the applied electrical field and the internal geometry of the polymer gels that could lead to improving biomolecule separation in gel electrophoresis.http://dx.doi.org/10.1155/2019/6194674 |
| spellingShingle | Jennifer A. Pascal Koteswara Rao Medidhi Mario A. Oyanader Holly A. Stretz Pedro E. Arce Understanding Collaborative Effects between the Polymer Gel Structure and the Applied Electrical Field in Gel Electrophoresis Separation International Journal of Polymer Science |
| title | Understanding Collaborative Effects between the Polymer Gel Structure and the Applied Electrical Field in Gel Electrophoresis Separation |
| title_full | Understanding Collaborative Effects between the Polymer Gel Structure and the Applied Electrical Field in Gel Electrophoresis Separation |
| title_fullStr | Understanding Collaborative Effects between the Polymer Gel Structure and the Applied Electrical Field in Gel Electrophoresis Separation |
| title_full_unstemmed | Understanding Collaborative Effects between the Polymer Gel Structure and the Applied Electrical Field in Gel Electrophoresis Separation |
| title_short | Understanding Collaborative Effects between the Polymer Gel Structure and the Applied Electrical Field in Gel Electrophoresis Separation |
| title_sort | understanding collaborative effects between the polymer gel structure and the applied electrical field in gel electrophoresis separation |
| url | http://dx.doi.org/10.1155/2019/6194674 |
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