Gleaning of δ15N in ecosystems and its animal-organ isotope data: perspectives for the construction of the isotopically ordered world
Abstract To date, empirical relationships have been observed for the distributions and variations of stable nitrogen and carbon isotope ratios (δ15N and δ13C, respectively) in ecosystems as well as in living organisms. Here, we investigated δ15N values of internal organs of various animal species, i...
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2025-08-01
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| Series: | Progress in Earth and Planetary Science |
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| Online Access: | https://doi.org/10.1186/s40645-025-00736-0 |
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| author | Eitaro Wada Yoshihisa Kudo Hiroshi Mizutani Hiroshi Minami Kenji Matsubara Yoshihiro Yamada Nanako O. Ogawa Yukihiro Kohmatsu Naoko Nishizawa Isamu Shimizu Reiichiro Ishii Fujio Hyodo Maki N. Aita Wataru Sakamoto Naohiko Ohkouchi |
| author_facet | Eitaro Wada Yoshihisa Kudo Hiroshi Mizutani Hiroshi Minami Kenji Matsubara Yoshihiro Yamada Nanako O. Ogawa Yukihiro Kohmatsu Naoko Nishizawa Isamu Shimizu Reiichiro Ishii Fujio Hyodo Maki N. Aita Wataru Sakamoto Naohiko Ohkouchi |
| author_sort | Eitaro Wada |
| collection | DOAJ |
| description | Abstract To date, empirical relationships have been observed for the distributions and variations of stable nitrogen and carbon isotope ratios (δ15N and δ13C, respectively) in ecosystems as well as in living organisms. Here, we investigated δ15N values of internal organs of various animal species, including insects, fish, amphibians, reptiles, birds, and mammals. The δ15N differences of these organs from the corresponding muscles vary within a range of ± 4‰. In general, the δ15N values of the brain and heart are higher than those of the corresponding muscles, except in birds. This may indicate that excreted ammonia is recycled in their cecum. We propose a kinetic descriptive schemas for a nitrogen isotope model of deamination that maintains a TDF of 8.0‰ for glutamate in muscle mitochondria. Differences in the δ15N in skeletal muscle, heart, and brain are evident across species because the cells that make up the organ differ greatly in shape and organization, and the amount and shape of mitochondria distributed in these organs change dynamically. These facts may be the first step to clarify the significance of organ δ15N variation for animal growth physiology and possibly for animal biochemical evolution. These evidences of δ15N variations at intact and organ level in biota may provide a new broad avenue to investigate paleoecology and its environmental conditions. |
| format | Article |
| id | doaj-art-147566fe92b84617901acd275410c5e0 |
| institution | Kabale University |
| issn | 2197-4284 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Progress in Earth and Planetary Science |
| spelling | doaj-art-147566fe92b84617901acd275410c5e02025-08-20T03:42:09ZengSpringerOpenProgress in Earth and Planetary Science2197-42842025-08-0112111810.1186/s40645-025-00736-0Gleaning of δ15N in ecosystems and its animal-organ isotope data: perspectives for the construction of the isotopically ordered worldEitaro Wada0Yoshihisa Kudo1Hiroshi Mizutani2Hiroshi Minami3Kenji Matsubara4Yoshihiro Yamada5Nanako O. Ogawa6Yukihiro Kohmatsu7Naoko Nishizawa8Isamu Shimizu9Reiichiro Ishii10Fujio Hyodo11Maki N. Aita12Wataru Sakamoto13Naohiko Ohkouchi14Mitsubishi-Kasei Institute of Life Sciences (Abolished)Mitsubishi-Kasei Institute of Life Sciences (Abolished)Mitsubishi-Kasei Institute of Life Sciences (Abolished)Faculty of Fisheries, Hokkaido UniversityCenter for Ecological Research, Kyoto UniversityCenter for Ecological Research, Kyoto UniversityCenter for Ecological Research, Kyoto UniversityCenter for Ecological Research, Kyoto UniversityCenter for Ecological Research, Kyoto UniversityCenter for Ecological Research, Kyoto UniversityJapan Agency for Marine-Earth Science TechnologyCenter for Ecological Research, Kyoto UniversityJapan Agency for Marine-Earth Science TechnologyKyoto UniversityJapan Agency for Marine-Earth Science TechnologyAbstract To date, empirical relationships have been observed for the distributions and variations of stable nitrogen and carbon isotope ratios (δ15N and δ13C, respectively) in ecosystems as well as in living organisms. Here, we investigated δ15N values of internal organs of various animal species, including insects, fish, amphibians, reptiles, birds, and mammals. The δ15N differences of these organs from the corresponding muscles vary within a range of ± 4‰. In general, the δ15N values of the brain and heart are higher than those of the corresponding muscles, except in birds. This may indicate that excreted ammonia is recycled in their cecum. We propose a kinetic descriptive schemas for a nitrogen isotope model of deamination that maintains a TDF of 8.0‰ for glutamate in muscle mitochondria. Differences in the δ15N in skeletal muscle, heart, and brain are evident across species because the cells that make up the organ differ greatly in shape and organization, and the amount and shape of mitochondria distributed in these organs change dynamically. These facts may be the first step to clarify the significance of organ δ15N variation for animal growth physiology and possibly for animal biochemical evolution. These evidences of δ15N variations at intact and organ level in biota may provide a new broad avenue to investigate paleoecology and its environmental conditions.https://doi.org/10.1186/s40645-025-00736-0Animal-organ δ15NStable isotope descriptive schemaFeeding dynamicsOxidative deamination |
| spellingShingle | Eitaro Wada Yoshihisa Kudo Hiroshi Mizutani Hiroshi Minami Kenji Matsubara Yoshihiro Yamada Nanako O. Ogawa Yukihiro Kohmatsu Naoko Nishizawa Isamu Shimizu Reiichiro Ishii Fujio Hyodo Maki N. Aita Wataru Sakamoto Naohiko Ohkouchi Gleaning of δ15N in ecosystems and its animal-organ isotope data: perspectives for the construction of the isotopically ordered world Progress in Earth and Planetary Science Animal-organ δ15N Stable isotope descriptive schema Feeding dynamics Oxidative deamination |
| title | Gleaning of δ15N in ecosystems and its animal-organ isotope data: perspectives for the construction of the isotopically ordered world |
| title_full | Gleaning of δ15N in ecosystems and its animal-organ isotope data: perspectives for the construction of the isotopically ordered world |
| title_fullStr | Gleaning of δ15N in ecosystems and its animal-organ isotope data: perspectives for the construction of the isotopically ordered world |
| title_full_unstemmed | Gleaning of δ15N in ecosystems and its animal-organ isotope data: perspectives for the construction of the isotopically ordered world |
| title_short | Gleaning of δ15N in ecosystems and its animal-organ isotope data: perspectives for the construction of the isotopically ordered world |
| title_sort | gleaning of δ15n in ecosystems and its animal organ isotope data perspectives for the construction of the isotopically ordered world |
| topic | Animal-organ δ15N Stable isotope descriptive schema Feeding dynamics Oxidative deamination |
| url | https://doi.org/10.1186/s40645-025-00736-0 |
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