Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum
Porphyridium cruentum microalgae have great potential to be developed as a source of active ingredients in various fields of health. It excretes secondary metabolites in the form of extracellular polysaccharides (EPS), potentially as antibacterial, antihyperglycemic, and immunostimulant components....
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2018-06-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/268 |
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| author | Nurul Mutmainnah Yenny Risjani Asus Maizar Suryanto Hertika |
| author_facet | Nurul Mutmainnah Yenny Risjani Asus Maizar Suryanto Hertika |
| author_sort | Nurul Mutmainnah |
| collection | DOAJ |
| description | Porphyridium cruentum microalgae have great potential to be developed as a source of active ingredients in various fields of health. It excretes secondary metabolites in the form of extracellular polysaccharides (EPS), potentially as antibacterial, antihyperglycemic, and immunostimulant components. This study aims to obtain the best culture density of P. cruentum, the active component of P. cruentum (EPS), and the chemical composition of EPS. The P. cruentum was cultivated on 15%, 20% and 25% culture stock, with an addition of silicate, Fe and vitamins in sterile seawater medium with salinity 35 ‰, pH 8, temperature 25-27°C, with continuous aeration and 2500 lux continuous 24 hours, for 14 days of culture period. The results of this study indicate differences in growth rates and abundance of microalgae to each culture stock. The 15%, 20% and 25% culture stocks each showed stationary phase at days 10th, 12th and 14th, with EPS concentrations of 10 ppt, 12 ppt, and 15 ppt, respectively. Variation of P. cruentum culture showed different EPS results, with a density of 25% capable of producing the highest EPS extract of 15,000 mg.L-1. The EPS is known to contain glucose and carboxylic acid compounds that can be utilized in the health and industrial fields. |
| format | Article |
| id | doaj-art-1471e3c5e81a429c83044cffb433ddde |
| institution | Kabale University |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2018-06-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-1471e3c5e81a429c83044cffb433ddde2025-08-20T03:47:24ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552018-06-018297102https://doi.org/10.21776/ub.jels.2018.008.02.05Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentumNurul Mutmainnah0Yenny Risjani1Asus Maizar Suryanto Hertika2Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaPorphyridium cruentum microalgae have great potential to be developed as a source of active ingredients in various fields of health. It excretes secondary metabolites in the form of extracellular polysaccharides (EPS), potentially as antibacterial, antihyperglycemic, and immunostimulant components. This study aims to obtain the best culture density of P. cruentum, the active component of P. cruentum (EPS), and the chemical composition of EPS. The P. cruentum was cultivated on 15%, 20% and 25% culture stock, with an addition of silicate, Fe and vitamins in sterile seawater medium with salinity 35 ‰, pH 8, temperature 25-27°C, with continuous aeration and 2500 lux continuous 24 hours, for 14 days of culture period. The results of this study indicate differences in growth rates and abundance of microalgae to each culture stock. The 15%, 20% and 25% culture stocks each showed stationary phase at days 10th, 12th and 14th, with EPS concentrations of 10 ppt, 12 ppt, and 15 ppt, respectively. Variation of P. cruentum culture showed different EPS results, with a density of 25% capable of producing the highest EPS extract of 15,000 mg.L-1. The EPS is known to contain glucose and carboxylic acid compounds that can be utilized in the health and industrial fields.https://jels.ub.ac.id/index.php/jels/article/view/268cultureextracellular polysaccharidesporphyridium cruentum |
| spellingShingle | Nurul Mutmainnah Yenny Risjani Asus Maizar Suryanto Hertika Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum Journal of Experimental Life Science culture extracellular polysaccharides porphyridium cruentum |
| title | Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum |
| title_full | Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum |
| title_fullStr | Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum |
| title_full_unstemmed | Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum |
| title_short | Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum |
| title_sort | growth rate and chemical composition of secondary metabolite extracellular polysaccharide eps in microalga porphyridium cruentum |
| topic | culture extracellular polysaccharides porphyridium cruentum |
| url | https://jels.ub.ac.id/index.php/jels/article/view/268 |
| work_keys_str_mv | AT nurulmutmainnah growthrateandchemicalcompositionofsecondarymetaboliteextracellularpolysaccharideepsinmicroalgaporphyridiumcruentum AT yennyrisjani growthrateandchemicalcompositionofsecondarymetaboliteextracellularpolysaccharideepsinmicroalgaporphyridiumcruentum AT asusmaizarsuryantohertika growthrateandchemicalcompositionofsecondarymetaboliteextracellularpolysaccharideepsinmicroalgaporphyridiumcruentum |