Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water- extractable colloids of a Vertisol

<p>Water-extractable colloids (WECs) serve as crucial micro-particulate components in soils, playing a vital role in the cycling and potential bioavailability of soil phosphorus (P). Yet, the underlying information regarding soil P species and P-transformation microorganisms at the micropartic...

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Main Authors: S. Bai, Y. Ge, D. Yao, Y. Wang, J. Tan, S. Zhang, Y. Peng, X. Jiang
Format: Article
Language:English
Published: Copernicus Publications 2025-01-01
Series:Biogeosciences
Online Access:https://bg.copernicus.org/articles/22/135/2025/bg-22-135-2025.pdf
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author S. Bai
S. Bai
Y. Ge
D. Yao
Y. Wang
J. Tan
J. Tan
S. Zhang
Y. Peng
X. Jiang
author_facet S. Bai
S. Bai
Y. Ge
D. Yao
Y. Wang
J. Tan
J. Tan
S. Zhang
Y. Peng
X. Jiang
author_sort S. Bai
collection DOAJ
description <p>Water-extractable colloids (WECs) serve as crucial micro-particulate components in soils, playing a vital role in the cycling and potential bioavailability of soil phosphorus (P). Yet, the underlying information regarding soil P species and P-transformation microorganisms at the microparticle scale under long-term straw retention and mineral fertilization is barely known. Here, a fixed field experiment (<span class="inline-formula">∼</span> 13 years) in a Vertisol was performed to explore the impacts of straw retention and mineral fertilization on inorganic P, organic P, and P-transformation microorganisms in bulk soils and WECs through a sequential extraction procedure, P K-edge X-ray absorption near-edge structure (XANES), <span class="inline-formula"><sup>31</sup></span>P nuclear magnetic resonance (NMR), and metagenomics analysis. In bulk soil, mineral fertilization led to increases in the levels of total P, available P, acid phosphatase (ACP), high-activity inorganic P fractions (Ca<span class="inline-formula"><sub>2</sub></span>-P, Ca<span class="inline-formula"><sub>8</sub></span>-P, Al-P, and Fe-P), and organic P (orthophosphate monoesters and orthophosphate diesters) but significantly decreased the abundances of P-cycling genes including P mineralization, P-starvation response regulation, and P uptake and transport by decreasing soil pH and increasing total P. Straw retention had no significant effects on P species and P-transformation microorganisms in bulk soils but led to increases in organic carbon, total P, and available P concentrations in WECs. Furthermore, compared with mineral fertilization, straw retention caused significantly greater differences in the relative abundances of P-cycling genes between WECs and bulk soils. The abundances of <i>phoD</i> gene and <i>phoD</i>-harboring Proteobacteria in WECs increased significantly under straw retention, suggesting that the P-mineralizing capacity increased. Thus, mineral fertilization reduced microbial P-solubilizing and mineralizing capacity in bulk soil. Straw retention could potentially accelerate the turnover, mobility, and availability of P by increasing the nutrient contents and P-mineralizing capacity at the microscopic colloidal scale.</p>
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spelling doaj-art-146ef151b8434c348fae5aa34868bf5b2025-08-20T02:27:36ZengCopernicus PublicationsBiogeosciences1726-41701726-41892025-01-012213515110.5194/bg-22-135-2025Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water- extractable colloids of a VertisolS. Bai0S. Bai1Y. Ge2D. Yao3Y. Wang4J. Tan5J. Tan6S. Zhang7Y. Peng8X. Jiang9School of Agriculture and Biotechnology, Sun Yat-sen University, Guangzhou, Guangdong 510275, PR ChinaInstitute of Eco-environment and Industrial Technology, Shanxi Agricultural University, Taiyuan, Shanxi 030031, PR ChinaSchool of Agriculture and Biotechnology, Sun Yat-sen University, Guangzhou, Guangdong 510275, PR ChinaSchool of Agriculture and Biotechnology, Sun Yat-sen University, Guangzhou, Guangdong 510275, PR ChinaSchool of Agriculture and Biotechnology, Sun Yat-sen University, Guangzhou, Guangdong 510275, PR ChinaSchool of Agriculture and Biotechnology, Sun Yat-sen University, Guangzhou, Guangdong 510275, PR ChinaInstitute of Eco-environment and Industrial Technology, Shanxi Agricultural University, Taiyuan, Shanxi 030031, PR ChinaKey Laboratory of Arable Land Quality Monitoring and Evaluation, State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, PR ChinaSchool of Agriculture and Biotechnology, Sun Yat-sen University, Guangzhou, Guangdong 510275, PR ChinaSchool of Agriculture and Biotechnology, Sun Yat-sen University, Guangzhou, Guangdong 510275, PR China<p>Water-extractable colloids (WECs) serve as crucial micro-particulate components in soils, playing a vital role in the cycling and potential bioavailability of soil phosphorus (P). Yet, the underlying information regarding soil P species and P-transformation microorganisms at the microparticle scale under long-term straw retention and mineral fertilization is barely known. Here, a fixed field experiment (<span class="inline-formula">∼</span> 13 years) in a Vertisol was performed to explore the impacts of straw retention and mineral fertilization on inorganic P, organic P, and P-transformation microorganisms in bulk soils and WECs through a sequential extraction procedure, P K-edge X-ray absorption near-edge structure (XANES), <span class="inline-formula"><sup>31</sup></span>P nuclear magnetic resonance (NMR), and metagenomics analysis. In bulk soil, mineral fertilization led to increases in the levels of total P, available P, acid phosphatase (ACP), high-activity inorganic P fractions (Ca<span class="inline-formula"><sub>2</sub></span>-P, Ca<span class="inline-formula"><sub>8</sub></span>-P, Al-P, and Fe-P), and organic P (orthophosphate monoesters and orthophosphate diesters) but significantly decreased the abundances of P-cycling genes including P mineralization, P-starvation response regulation, and P uptake and transport by decreasing soil pH and increasing total P. Straw retention had no significant effects on P species and P-transformation microorganisms in bulk soils but led to increases in organic carbon, total P, and available P concentrations in WECs. Furthermore, compared with mineral fertilization, straw retention caused significantly greater differences in the relative abundances of P-cycling genes between WECs and bulk soils. The abundances of <i>phoD</i> gene and <i>phoD</i>-harboring Proteobacteria in WECs increased significantly under straw retention, suggesting that the P-mineralizing capacity increased. Thus, mineral fertilization reduced microbial P-solubilizing and mineralizing capacity in bulk soil. Straw retention could potentially accelerate the turnover, mobility, and availability of P by increasing the nutrient contents and P-mineralizing capacity at the microscopic colloidal scale.</p>https://bg.copernicus.org/articles/22/135/2025/bg-22-135-2025.pdf
spellingShingle S. Bai
S. Bai
Y. Ge
D. Yao
Y. Wang
J. Tan
J. Tan
S. Zhang
Y. Peng
X. Jiang
Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water- extractable colloids of a Vertisol
Biogeosciences
title Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water- extractable colloids of a Vertisol
title_full Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water- extractable colloids of a Vertisol
title_fullStr Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water- extractable colloids of a Vertisol
title_full_unstemmed Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water- extractable colloids of a Vertisol
title_short Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water- extractable colloids of a Vertisol
title_sort effect of straw retention and mineral fertilization on p speciation and p transformation microorganisms in water extractable colloids of a vertisol
url https://bg.copernicus.org/articles/22/135/2025/bg-22-135-2025.pdf
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