In vitro validation of a method for neonatal urine collection and analysis
Objective Urine collection and analysis is important for diagnosis, monitoring of clinical progress, and research in neonates. This study aims to validate a novel methodology for neonatal urine collection, which combines the convenience of cotton ball collection with accurate timing via a urine cont...
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| Format: | Article |
| Language: | English |
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BMJ Publishing Group
2019-09-01
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| Series: | BMJ Paediatrics Open |
| Online Access: | https://bmjpaedsopen.bmj.com/content/3/1/e000482.full |
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| author | David M Reith Natalie Medlicott Roland Broadbent Darren Ritchie |
| author_facet | David M Reith Natalie Medlicott Roland Broadbent Darren Ritchie |
| author_sort | David M Reith |
| collection | DOAJ |
| description | Objective Urine collection and analysis is important for diagnosis, monitoring of clinical progress, and research in neonates. This study aims to validate a novel methodology for neonatal urine collection, which combines the convenience of cotton ball collection with accurate timing via a urine continence monitor.Design Laboratory model using a combined cotton ball and urinary incontinence monitor method with and without the presence of an impermeable membrane to prevent desiccation.Main outcome measures Accuracy, bias and precision in measurement of urine volume, electrolytes (sodium, potassium, chloride), creatinine and gentamicin. Changes in analyte concentration over time, and evaporative loss of water, were tested using analysis of variance. The effects of time, temperature and humidity were explored using multivariate analysis of variance.Results With the use of an impermeable membrane, sodium concentration increased from a mean (SD) of 3.57% (0.68) at 1 min to 5.03% (0.74) at 120 min. There was no significant change in potassium, chloride or creatinine concentrations. Gentamicin concentration decreased by a mean (SD) of 9.05% (1.37) by 30 min. Multivariate analysis found that absolute change in weight, sodium and chloride were only dependent on duration. Gentamicin concentration was affected by duration, humidity and temperature. Relative evaporative loss was minimal at −0.58% (0.31), and the urinary continence monitor was 100% successful at detecting urination for all time points.Conclusions This novel methodology provides a standardisable and practical method to collect small volumes of neonatal urine for accurate measurement of both urine output and analyte concentrations. |
| format | Article |
| id | doaj-art-14623adaba8c488ab3b3f9a5c2b22d07 |
| institution | Kabale University |
| issn | 2399-9772 |
| language | English |
| publishDate | 2019-09-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | BMJ Paediatrics Open |
| spelling | doaj-art-14623adaba8c488ab3b3f9a5c2b22d072024-12-01T09:25:09ZengBMJ Publishing GroupBMJ Paediatrics Open2399-97722019-09-013110.1136/bmjpo-2019-000482In vitro validation of a method for neonatal urine collection and analysisDavid M Reith0Natalie Medlicott1Roland Broadbent2Darren Ritchie31 Department of Women’s and Children’s Health, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand2 School of Pharmacy, University of Otago, Dunedin, New Zealand1 Department of Women’s and Children’s Health, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand1 Department of Women’s and Children’s Health, Dunedin School of Medicine, University of Otago, Dunedin, New ZealandObjective Urine collection and analysis is important for diagnosis, monitoring of clinical progress, and research in neonates. This study aims to validate a novel methodology for neonatal urine collection, which combines the convenience of cotton ball collection with accurate timing via a urine continence monitor.Design Laboratory model using a combined cotton ball and urinary incontinence monitor method with and without the presence of an impermeable membrane to prevent desiccation.Main outcome measures Accuracy, bias and precision in measurement of urine volume, electrolytes (sodium, potassium, chloride), creatinine and gentamicin. Changes in analyte concentration over time, and evaporative loss of water, were tested using analysis of variance. The effects of time, temperature and humidity were explored using multivariate analysis of variance.Results With the use of an impermeable membrane, sodium concentration increased from a mean (SD) of 3.57% (0.68) at 1 min to 5.03% (0.74) at 120 min. There was no significant change in potassium, chloride or creatinine concentrations. Gentamicin concentration decreased by a mean (SD) of 9.05% (1.37) by 30 min. Multivariate analysis found that absolute change in weight, sodium and chloride were only dependent on duration. Gentamicin concentration was affected by duration, humidity and temperature. Relative evaporative loss was minimal at −0.58% (0.31), and the urinary continence monitor was 100% successful at detecting urination for all time points.Conclusions This novel methodology provides a standardisable and practical method to collect small volumes of neonatal urine for accurate measurement of both urine output and analyte concentrations.https://bmjpaedsopen.bmj.com/content/3/1/e000482.full |
| spellingShingle | David M Reith Natalie Medlicott Roland Broadbent Darren Ritchie In vitro validation of a method for neonatal urine collection and analysis BMJ Paediatrics Open |
| title | In vitro validation of a method for neonatal urine collection and analysis |
| title_full | In vitro validation of a method for neonatal urine collection and analysis |
| title_fullStr | In vitro validation of a method for neonatal urine collection and analysis |
| title_full_unstemmed | In vitro validation of a method for neonatal urine collection and analysis |
| title_short | In vitro validation of a method for neonatal urine collection and analysis |
| title_sort | in vitro validation of a method for neonatal urine collection and analysis |
| url | https://bmjpaedsopen.bmj.com/content/3/1/e000482.full |
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