Inhibition of Horse Liver Alcohol Dehydrogenase by Methyltin Compounds
The study of inorganic tin (SnCl2, SnCl4) and methyltin compounds (MeSnCl3, Me2SnCI2, Me3SnCl) effects on the enzymatic activity of alcohol dehydrogenase (ADH) in the reaction of ethanol oxidation has been carried out. The experimental results of the study show that inorganic tin and methyltin subst...
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| Format: | Article |
| Language: | English |
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Wiley
2005-01-01
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| Series: | Bioinorganic Chemistry and Applications |
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| Online Access: | http://dx.doi.org/10.1155/BCA.2005.191 |
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| author | Pavel V. Bychkov Tatyana N. Shekhovtsova Elena R. Milaeva |
| author_facet | Pavel V. Bychkov Tatyana N. Shekhovtsova Elena R. Milaeva |
| author_sort | Pavel V. Bychkov |
| collection | DOAJ |
| description | The study of inorganic tin (SnCl2, SnCl4) and methyltin compounds (MeSnCl3, Me2SnCI2, Me3SnCl)
effects on the enzymatic activity of alcohol dehydrogenase (ADH) in the reaction of ethanol oxidation has
been carried out. The experimental results of the study show that inorganic tin and methyltin substances
induce slight inhibition of the catalytic activity of horse liver alcohol dehydrogenase (HLADH), unable to be
improved during pre-incubation with the enzyme. The conditions for carrying out the kinetic investigation of
the mentioned phenomenon were optimized and as it turned out the mechanism of methyltin trichloride
action, as the most effective methyltin inhibitor, is more complex than the proposed interaction of the metal
atom with SH-groups of the enzyme protein. It was demonstrated that the tin compounds act in the same
manner as methylmercury compounds and might serve as oxidative agents towards the co-enzyme NADH.
Kinetic data on MeSnCl3 were calculated. Data acquired on NAD-dependent ADH from horse liver and those
regarding NAD-dependent LDH from sturgeon liver were compared. |
| format | Article |
| id | doaj-art-145fc7191aa6492dbe8554f468d66e31 |
| institution | OA Journals |
| issn | 1565-3633 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-145fc7191aa6492dbe8554f468d66e312025-08-20T02:04:52ZengWileyBioinorganic Chemistry and Applications1565-36332005-01-0133-419119910.1155/BCA.2005.191Inhibition of Horse Liver Alcohol Dehydrogenase by Methyltin CompoundsPavel V. Bychkov0Tatyana N. Shekhovtsova1Elena R. Milaeva2M.V. Lomonosov Moscow State University, Chemistry Department, Leninskie Gory, Moscow 119992, RussiaM.V. Lomonosov Moscow State University, Chemistry Department, Leninskie Gory, Moscow 119992, RussiaM.V. Lomonosov Moscow State University, Chemistry Department, Leninskie Gory, Moscow 119992, RussiaThe study of inorganic tin (SnCl2, SnCl4) and methyltin compounds (MeSnCl3, Me2SnCI2, Me3SnCl) effects on the enzymatic activity of alcohol dehydrogenase (ADH) in the reaction of ethanol oxidation has been carried out. The experimental results of the study show that inorganic tin and methyltin substances induce slight inhibition of the catalytic activity of horse liver alcohol dehydrogenase (HLADH), unable to be improved during pre-incubation with the enzyme. The conditions for carrying out the kinetic investigation of the mentioned phenomenon were optimized and as it turned out the mechanism of methyltin trichloride action, as the most effective methyltin inhibitor, is more complex than the proposed interaction of the metal atom with SH-groups of the enzyme protein. It was demonstrated that the tin compounds act in the same manner as methylmercury compounds and might serve as oxidative agents towards the co-enzyme NADH. Kinetic data on MeSnCl3 were calculated. Data acquired on NAD-dependent ADH from horse liver and those regarding NAD-dependent LDH from sturgeon liver were compared.http://dx.doi.org/10.1155/BCA.2005.191alcohol dehydrogenasemethyltin compoundsNADinhibition type. |
| spellingShingle | Pavel V. Bychkov Tatyana N. Shekhovtsova Elena R. Milaeva Inhibition of Horse Liver Alcohol Dehydrogenase by Methyltin Compounds Bioinorganic Chemistry and Applications alcohol dehydrogenase methyltin compounds NAD inhibition type. |
| title | Inhibition of Horse Liver Alcohol Dehydrogenase by
Methyltin Compounds |
| title_full | Inhibition of Horse Liver Alcohol Dehydrogenase by
Methyltin Compounds |
| title_fullStr | Inhibition of Horse Liver Alcohol Dehydrogenase by
Methyltin Compounds |
| title_full_unstemmed | Inhibition of Horse Liver Alcohol Dehydrogenase by
Methyltin Compounds |
| title_short | Inhibition of Horse Liver Alcohol Dehydrogenase by
Methyltin Compounds |
| title_sort | inhibition of horse liver alcohol dehydrogenase by methyltin compounds |
| topic | alcohol dehydrogenase methyltin compounds NAD inhibition type. |
| url | http://dx.doi.org/10.1155/BCA.2005.191 |
| work_keys_str_mv | AT pavelvbychkov inhibitionofhorseliveralcoholdehydrogenasebymethyltincompounds AT tatyananshekhovtsova inhibitionofhorseliveralcoholdehydrogenasebymethyltincompounds AT elenarmilaeva inhibitionofhorseliveralcoholdehydrogenasebymethyltincompounds |