Long-read sequencing to detect full-length protein–protein interactions

Long-read sequencing to detect full-length protein–protein interactions

Abstract Given the increased predictions on interactome size and demand for protein function information, methods for detecting protein-protein interactions remain a significant development area. The all-vs.-all sequencing (AVA-Seq) method utilizes a convergent fusion plasmid design to make two-hybr...

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Bibliographic Details
Main Authors: Stephanie Schaefer-Ramadan, Yue Guan, Ayeda A. Ahmed, Jovana Aleksic, Khadija A. Elmagarmid, Leena F. Syed, Yasmin A. Mohamoud, Joel A. Malek
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
Subjects:
Oxford nanopore technologies
Long-read sequencing
Protein–protein interaction
Two-hybrid
Single-molecule sequencing
Online Access:https://doi.org/10.1038/s41598-025-08549-3
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